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Mechanisms underlying integration of synaptic plasticity in neuronal networks

Research Project

Project/Area Number 16300150
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionThe University of Tokyo

Principal Investigator

JIMBO Yasuhiko  The University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (20372401)

Co-Investigator(Kenkyū-buntansha) YASUDA Kenji  Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Professor, 生体材料工学研究所, 教授 (20313158)
SAKUMA Ichiro  The University of Tokyo, Graduate School of Engineering, Professor, 大学院・工学系研究科, 教授 (50178597)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2005: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥9,500,000 (Direct Cost: ¥9,500,000)
Keywordsplasticity / cell culture / neuron / electrode array / electrical stimulation / neuronal network
Research Abstract

Activity-dependent modification of synaptic connections probably plays a key role in both proper network formation during development and learning and memory. Based on the capability of multi-site stimulation, MEA recording was applied to see modification of evoked responses induced by repetitive application of temporally correlated stimuli. Cortical neurons show developmental changes in spontaneous activity and reach the steady state after about one month in vitro. Evoked responses also depend on their developmental stages. Thus, in this work, matured cultures of more than 40 days in vitro were used for recording. A series of recording consists of (1) evoked responses to test stimuli and (2) responses to the correlated stimuli. The test stimuli were composed of 100 times of single pulse stimulus from site A and another 100 times from site B. Each single-site stimulation was applied every six seconds. The correlated stimuli consisted of 100 times of double pulses. The first pulse applied from site A was followed by the second pulse from the site B with a fixed time delay. Time delays of 100, 50, 20, 10, and 0 ms were tested. The total process of the experiment was composed of 6 sets of test stimuli and 5 sets of correlated stimuli: test1-A&B100-test2-A&B50-test3-A&B20-test4-A&B10-tests-A&B0-test6. The evoked activity consisted of early and late components. At the initial state, almost no late components were recorded. After the correlated stimuli, strong late components appeared. The late components were detected after about 30 ms, and 20 ms, respectively. To analyze relationship between induced activity-changes and the applied correlated stimuli, cross-correlation functions were calculated. These results suggested that the properties of the applied correlated stimuli might be reflected in the spatio-temporal structures in the evoked responses.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (32 results)

All 2007 2006 2005 2004

All Journal Article (29 results) Book (3 results)

  • [Journal Article] MEA-based recording of neuronal activity in vitro2007

    • Author(s)
      Y.Jimbo
    • Journal Title

      Arch. Ita. Biol. 127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MEA-based recording of neuronal activity in vitro2007

    • Author(s)
      Y.Jimbo
    • Journal Title

      Arch. Ita. Biol. 127(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] スブレーパターニングと可動式微小電極による小規模神経回路の位置選択的自発活動計測2007

    • Author(s)
      森口, 玉井, 高山, 倉島, 神保
    • Journal Title

      電気学会電子情報システム部門誌 127(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The state-of art of NanobioScience in Japan2006

    • Author(s)
      S.UenoJ, J.Ando, H.Fujita, T.Sugawara, Y.Jimbo, K.Itaka, K.Kataoka, T.Ushida
    • Journal Title

      IEEE Trans. Nanobiosci. 5

      Pages: 54-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The state-of art of NanobioScience in Japan2006

    • Author(s)
      S.Ueno, J.Ando, H.Fujita, T.Sugawara, Y.Jimbo, K.Itaka K.Kataoka, T.Ushida
    • Journal Title

      IEEE Trans. Nanobiosci. 5

      Pages: 54-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MEA-based recording and analysis of neuronal network activity2006

    • Author(s)
      Y.Jimbo
    • Journal Title

      in H.Kambara, T.Matsunaga, M.Ueda Eds., Frontier of Quantitative Analysis of A Single Cell

      Pages: 175-187

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MEA-based spike recording in cultured neuronal networks2006

    • Author(s)
      Y.Jimbo, N.Kasai, K.Torimitsu, T.Tateno
    • Journal Title

      in Wan-Li Xing and Jing Cheng Eds., Frontiers in Biochip Technology (Springer-Verlag)

      Pages: 88-98

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The state-of art of NanobioScience in Japan2006

    • Author(s)
      S.Ueno, J.Ando, H.Fujita, T.Sugawara, Y.Jimbo, K..Itaka, K.Kataoka, T.Ushida
    • Journal Title

      IEEE Trans. Nanobiosci. 5

      Pages: 54-65

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The state-of art of NanobioScience in Japan2006

    • Author(s)
      S.Ueno, J.Ando, H.Fujita, T.Sugawara, Y.Jimbo, K.Itaka, K.Kataoka, T.Ushida
    • Journal Title

      IEEE Trans.Nanobioscience 5

      Pages: 1-12

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of ratcortical neurons : evoked activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neurosci. 134

      Pages: 439-448

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of ratcortical neurons : spontaneous activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neurosci. 134

      Pages: 425-437

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 大脳皮質神経回路形成過程における細胞内Caダイナミクス2005

    • Author(s)
      高山, 森本, 郷, 佐久間, 小林, 神保
    • Journal Title

      豊田研究報告 58

      Pages: 71-77

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Stepwise pattern modification of neuronal network in photo-thermally-etched agarose architecture on multi-electrode array chip for single-cell-based electrophysiological measurement2005

    • Author(s)
      I.Suzuki, Y.Sugio, Y.Jimbo, K.Yasuda
    • Journal Title

      Lab Chip 5

      Pages: 241-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of rat cortical neurons : evoked activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neurosci. 134

      Pages: 439-448

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of rat cortical neurons : spontaneous activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neurosci. 134

      Pages: 425-437

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sevelopmental changes of Ca dynamics in cultured cortical networks2005

    • Author(s)
      Y.Takayama, K.Morimoto, S.Go, E.Kobayashi, I.Sakuma, Y.Jimbo
    • Journal Title

      Rep. Toyota Phys. Chem. Res. Inst. 58

      Pages: 71-77

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of rat cortical neurons : evoked activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neuroscience 134

      Pages: 439-448

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spatio-temporal cholinergic modulation in cultured networks of rat cortical neurons : spontaneous activity2005

    • Author(s)
      T.Tateno, Y.Jimbo, H.P.C.Robinson
    • Journal Title

      Neuroscience 58

      Pages: 425-437

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stepwise pattern modification of neuronal network in photo-thermally-etched agarose architecture on multi-electrode array chip for single-cell-based electrophysiological measurement2005

    • Author(s)
      I.Suzuki, Y.Sugio, Y.Jimbo, K.Yasuda
    • Journal Title

      Lab on Chip 5

      Pages: 241-247

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stepwise pattern modification of neuronal network in photo-thermally-etched agarose architecture on MEA chip for electrophysiological measurement2005

    • Author(s)
      I.Suzuki, Y.Jimbo他
    • Journal Title

      Lab Chip 5(3)

      Pages: 241-247

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pattern modification of a neuronal network for individual-cell-based electrophysiological measurement using photo-thermal etching of an agarose architecture with a multi-electrode array2004

    • Author(s)
      I.Suzuki, Y.Sugio, H.Moriguchi, A.Hattori, K.Yasuda, Y.Jimbo
    • Journal Title

      IEE Proc. Nanobiotechnol. 151

      Pages: 116-121

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Modification of a neuronal network direction using stepwise photo-thermal etching of an agarose architecture2004

    • Author(s)
      I.Suzuki, Y.Sugio, H.Moriguchi, Y.Jimbo, K.Yasuda
    • Journal Title

      J. Nanobiotechnol. 2

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Individual-cell-based electrophysiological measurement of a topographically controlled neuronal network pattern using agarose architecture with a multi-electrode array2004

    • Author(s)
      I.Suzuki, Y.Sugio, Y.Jimbo, K.Yasuda
    • Journal Title

      Jpn. J. Appl. Phys. 43

    • NAID

      10012705129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Individual-cell-base electrophysiological measurement of a topographically controlled neuronal network pattern using agarose architecture with a multi-electrode array2004

    • Author(s)
      I.Suzuki, Y.Sugio, Y.Jimbo, K.Yasuda
    • Journal Title

      Jpn. J. Appl. Phys. 43

    • NAID

      10012705129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] On-chip neural cell cultivation using agarose-microchamber array constructed by a photothermal etching method2004

    • Author(s)
      H.Moriguchi, Y.Jimbo他
    • Journal Title

      Electrical Engineering in Japan 146(2)

      Pages: 37-42

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pattern modification of a neuronal network for individual-cell-based electrophysiol. measurement using photothermal etching of an agarose architecture2004

    • Author(s)
      I.Suzuki, Y.Jimbo他
    • Journal Title

      IEE Proceedings Nanobiotechnology 151(3)

      Pages: 116-121

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Individual-cell-based electrophysiological measurement of a topographically controlled neuronal network pattern using agarose architecture2004

    • Author(s)
      I.Suzuki, Y.Jimbo他
    • Journal Title

      Japanese Journal of Applied Physics 43(3)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Modification of neuronal network direction using stepwise photo-thermal etching of an agarose architecture2004

    • Author(s)
      I.Suzuki, Y.Jimbo他
    • Journal Title

      Journal of Nanobiotechnology 2:7

    • Related Report
      2004 Annual Research Report
  • [Journal Article] in vitro系の多点電気刺激2004

    • Author(s)
      神保
    • Journal Title

      BME 18(4)

      Pages: 43-47

    • NAID

      10014206942

    • Related Report
      2004 Annual Research Report
  • [Book] 一細胞定量解析の最前線2006

    • Author(s)
      神保
    • Publisher
      CMC出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Book] Frontiers in Biochip Technology2006

    • Author(s)
      Y.Jimbo, N.Kasai, K.Torimitsu, T.Tateno
    • Publisher
      Springer-Verlag
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Book] Frontiers in Biochip Technology (分担執筆)2006

    • Author(s)
      Y.Jimbo
    • Publisher
      Springer-Verlag (in press)
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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