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Development of Photonic-imaging System with Fluorescent and Phosphorescent Nano-probes

Research Project

Project/Area Number 16300160
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKeio University

Principal Investigator

HINAMITANI Haruyuki  Keio University, Faculty of Science and Technology, Professor, 理工学部, 教授 (70051779)

Co-Investigator(Kenkyū-buntansha) TANISHITA Kazuo  Keio University, Faculty of Science and Technology, Professor, 理工学部, 教授 (10101776)
SUEMATSU Makoto  Keio University, School of Medicine, Professor, 医学部, 教授 (00206385)
KAWANISHI Tooru  National Institute of Health Science, Department of Pharmacology, Senior Director, 生物薬品部, 部長 (40124383)
関塚 永一  国立病院機構, 埼玉病院, 副院長
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2004: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsBioimaging / Nano-probes / Fluorescense / Phosphorescence / Microcirculation / Blood flow / Oxygen tension / Pd-porphyrin / 螢光 / 糖尿病
Research Abstract

Multi-colored photonic imaging system was developed for analyzing molecular and physiological functions in mammalian cells and organ tissues in connection with novel utilization of various fluorescent and phosphorescent nano-probes. Simultaneous measurement of blood flow and oxygen tension was enabled by using fluorescent isothiocyanate labeled erythrocytes and Pd-meso-tetra-(4-carboxyphenyl)-porphyrin in the microvessels of brain, liver and tumor tissues under the intravital and inverted microscopes. Furthermore, endothelial cell-platelet-leukocyte-interactions were observed to elucidate the mechanism of ischemia-reperfusion injury as well as diabetic condition and reactive oxygen exposure. Reaction of hypoxia-inducible factor (HIF)-1 regulating NO and CO gas molecule expression was also analyzed by means of biochemical methods and metabolome analysis. On the other hand, dynamics of caspase family and calcium ion as well as degeneration of cellular proteins in the vascular endothelial cells, hepatocytes, cardiac cells, tumor cells were analyzed by means of in-vitro fluorescent visualization to elucidate the molecular and physiological functions related with apoptosis process, atherosclerosis growth, angiogenesis-vasculogenesis development. These observations gave us various significant findings concerned in the vascular diseases of diabetes, hypertension, tumor incision and so on.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (44 results)

All 2007 2006 2005 2004

All Journal Article (43 results) Book (1 results)

  • [Journal Article] Up-regulation of adhesion molecule expression in glomerular endothelial cells by anti-myeloperoxidase antibody.2007

    • Author(s)
      Nagao T., Matsumura M., Suzuki K., Minamitani H., ら
    • Journal Title

      Nephrology Dialysis Transplantation 22

      Pages: 77-87

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Angiotensin II type 1 receptor signaling contributes to platelet-leukocyte-endothelial cell interactions in the cerebral microvasculature.2007

    • Author(s)
      Ishikawa M, Sekizuka E, Nakadate H, Minamitani Hら
    • Journal Title

      American Journal of Physiology, Heart and Circulatory Physiology 292・5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of hemoglobin allostery in hypoxia-induced metabolic alterations in erythrocytes : simulation and its verification by metabolome analysis.2007

    • Author(s)
      Kinoshita A, Tsukada K, Soga T, Tomita M, Suematsu M, ら
    • Journal Title

      Journal of Biological Chemistry 282・14

      Pages: 10731-10741

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Caspase cascade proceeds rapidly after cytochrome c release from mitochondria in tumor necrosis factor-alpha-induced cell death.2007

    • Author(s)
      Kawai H, Suzuki T, Kobayashi T, Momose K, Hayakawa T, Kawanishi Tら
    • Journal Title

      Journal of Pharmacological Science 103

      Pages: 159-167

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of adhesion molecule expression in glomerular endothelial cells by anti-myeloperoxidase antibody.2007

    • Author(s)
      Nagao T, Matsumura M, Mabuchi A, Koshio 0, Nakayama T, Minamitani H, Suzuki K.
    • Journal Title

      Nephrology Dialysis Transplantation 22

      Pages: 77-87

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Angiotensin II type 1 receptor signaling contributes to platelet-leukocyte-endothelial cell interactions in the cerebral microvasculature.2007

    • Author(s)
      Ishikawa M, Sekizuka E, Yamaguchi N, Nakadate H, Terao S, Granger DN, Minamitani H
    • Journal Title

      American Journal of Physiology, Heart and Circulatory Physiology 292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of hemoglobin allostery in hypoxia-induced metabolic alterations in erythrocytes : simulation and its verification by metabolome analysis.2007

    • Author(s)
      Kinoshita A, Tsukada K, Soga T, Hishiki T, Ueno Y, Nakayama Y, Tomita M, Suematsu M
    • Journal Title

      Journal of Biological Chemistry 282(14)

      Pages: 10731-10741

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Caspase cascade proceeds rapidly after cytochrome c release from mitochondria in tumor necrosis factor-alpha-induced cell death.2007

    • Author(s)
      Kawai H, Suzuki T, Kobayashi T, Ishii-Watabe A, Sakurai H, Ohata H, Honda K, Momose K, Hayakawa T, Kawanishi T
    • Journal Title

      J Pharmacol ScL. 103

      Pages: 159-67

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of adhesion molecule expression in glomerular endothelial cells by anti-myeloperoxidase antibody2007

    • Author(s)
      Nagao T., Matsumura M., Suzuki K., Nakayama T., Minamitani H., ら
    • Journal Title

      Nephrology Dialysis Transplantation 22

      Pages: 77-67

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Angiotensin II type 1 receptor signaling contributes to platelet-leukocyte-endothelial cell interactions in the cerebral microvasculature2007

    • Author(s)
      Ishikawa M, Sekizuka E, Nakadate H, Yamaguchi N, Minamitani H ら
    • Journal Title

      American Journal of Physiology, Heart and Circulatory Physiology 292(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Roles of hemoglobin allostery in hypoxia-induced metabolic alterations in erythrocytes : simulation and its verification by metabolome analysis2007

    • Author(s)
      Kinoshita A, Tsukada K, Soga T, Tomita M, Suematsu M, ら
    • Journal Title

      Journal of Biological Chemistry 282(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Initial bFGF distribution affects the depth of three-dimensional microvessel networks in vitro.2006

    • Author(s)
      Ueda A, Sudo R, Ikeda M, Kudo S, Tanishita K
    • Journal Title

      Journal of Biomechanical Science and Engineering 1・1

      Pages: 136-146

    • NAID

      130000079537

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Erythrocytes with T-state-stabilized hemoglobin as a therapeutic tool for post-ischemic liver dysfunction.2006

    • Author(s)
      Suganuma K, Tsukada K, Goda N, Kitajima M, Suematsu M, ら
    • Journal Title

      Antioxidants and Redox Signaling 8

      Pages: 1847-1855

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative fluorescence measurement of cardiac Na^+/Ca^+ exchanger inhibition by kinetic analysis in stably transfected HEK293 cells.2006

    • Author(s)
      Namekata I, Kawanishi T, Iida-Tanaka H, Tanaka H, Shigenobu K
    • Journal Title

      Journal of Pharmacological Science 101

      Pages: 356-360

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reduction by SEA0400 of myocardial ischemia-induced cytoplasmic and mitochondrial Ca^<2+> overload.2006

    • Author(s)
      Namekata I, Shimada H, Kawanishi T, Tanaka H, Shigenobu K
    • Journal Title

      European Journal of Pharmacology 543

      Pages: 108-115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Initial bFGF distribution affects the depth of three-dimensional microvessel networks in vitro.2006

    • Author(s)
      Ueda A, Sudo R, Ikeda M, Kudo S, Tanishita K
    • Journal Title

      J Biomechanical Science and Engineering 1(1)

      Pages: 136-146

    • NAID

      130000079537

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Erythrocytes with T-state stabilized hemoglobin as a therapeutic tool for postischemic liver dysfunction.2006

    • Author(s)
      Suganuma K, Tsukada K, Tsuneshige A, Kashiba M, Yonetani T, Suematsu M
    • Journal Title

      Antioxid. Redox Signaling 8(9)

      Pages: 1847-1855

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reduction by SEA0400 of myocardial ischemia-induced cytoplasmic and mitochondrial Ca2+ overload.2006

    • Author(s)
      Namekata I, Shimada H, Kawanishi T, Tanaka H, Shigenobu K
    • Journal Title

      Eur J PharmacoL 543

      Pages: 108-115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative fluorescence measurement of cardiac Na+/Ca+ exchanger inhibition by kinetic analysis in stably transfected HEK293 cells.2006

    • Author(s)
      Namekata I, Kawanishi T, Iida-Tanaka H, Tanaka H, Shigenobu K
    • Journal Title

      J. Pharmacol. ScL. 101

      Pages: 356-360

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] マルチカラーイメージングを用いた血液流動性測定における新しい観察法2006

    • Author(s)
      中楯浩康, 内田祥太, 石川真実, 山口則之, 大塩力, 南谷晴之, 関塚永一
    • Journal Title

      Hemorheology and Related Research(日本ヘモレオロジー学会誌) 9・2

      Pages: 11-14

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Initial bFGF distribution affects the depth of three-dimensional microvessel networks in vitro2006

    • Author(s)
      Ueda A, Sudo R, Ikeda M, Kudo S, Tanishita K.
    • Journal Title

      Journal of Biomechanical Science and Engineering 1・1

      Pages: 136-146

    • NAID

      130000079537

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Erythrocytes with T-state-stabilized hemoglobin as a therapeutic tool for post-ischemic liver dysfunction2006

    • Author(s)
      Suganuma K, Tsukada K, Goda N, Kitajima M, Suematsu M, ら
    • Journal Title

      Antioxidants and Redox Signaling 8

      Pages: 1847-55

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Erythrocytes with T-state-stabilized Hemoglobin as a Therapeutic Tool for Post-ischemic Liver Dysfunction2006

    • Author(s)
      K.Suganuma, K.Tsukada, M.Suematsu
    • Journal Title

      Antioxidants and Redox Signaling (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Coordinated movement of bile canalicular networks reconstructed by rat small hepatocytes.2005

    • Author(s)
      Sudo R, Kohara H, Mitaka T, Ikeda M, Tanishita, K
    • Journal Title

      Annals of BiomedicalEngineering 33・5

      Pages: 696-708

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Carbon monoxide from heme oxygenase-2 is a tonic regulator against nitricoxide-dependent vasodilatation in the adult rat cerebral microcirculation.2005

    • Author(s)
      Ishikawa M, Kajimura M, Adachi T, Goda N, T, Sekizuka E, Suematsu Mら
    • Journal Title

      Circulation Research 97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coordinated movement of bile canalicular networks reconstructed by rat small hepatocytes.2005

    • Author(s)
      Sudo R, Kohara H, Mitaka T, Ikeda, M Tanishita, K
    • Journal Title

      Annals ofBiomedEng. 33(5)

      Pages: 696-708

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Carbon monoxide from heme oxygenase-2 is a tonic regulator against nitric oxide-dependent vasodilatation in the adult rat cerebral microcirculation.2005

    • Author(s)
      Ishikawa M, Kajimura M, Adachi T, Maruyama K, Makino N, Goda N, Yamaguchi T, Sekizuka E, Suematsu M
    • Journal Title

      Circulation Research 97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dysfunction of Endothelial Cell under High Glucose and Static Pressure Condition2005

    • Author(s)
      Y.Hirose, E.Sekizuka, H.Minamitani
    • Journal Title

      Microcirculation Annual 21

      Pages: 83-84

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Simultaneous Analysis of Leukocyte-Platelet Dynamics in Cerebral Ischemia and Reperfusion by Use of the Multicolor Imaging2005

    • Author(s)
      N.Suzuki, E.Sekizuka, H.Minamitani
    • Journal Title

      Microcirculation Annual 21

      Pages: 45-46

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Carbon monoxide as a Guardian against Hepatobiliary Dysfunction2005

    • Author(s)
      M.Suematsu, K.Tsukada, T.Tajima
    • Journal Title

      Alcoholism, Clinical and Experimental Research 29, 11

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Red blood cell velocity and oxygen tension measurement in cerebral microvessels by double-wavelength photoexcitation.2004

    • Author(s)
      Tsukada K, Sekizuka E, Oshio C, Tsujioka K, Minamitani H
    • Journal Title

      Journal of Applied Physiology 96

      Pages: 1561-1568

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Photonic imaging analysis of blood flow and oxygen tension in organ microcirculation.2004

    • Author(s)
      Minamitani H, Tsukada K
    • Journal Title

      Journal of Clinical and Experimental Medicine 210・3

      Pages: 200-204

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relationship between microtubule network structure and intracellular transport in cultured endothelial cells affected by shear stress.2004

    • Author(s)
      Kudo S, Ikezawa K, Ikeda M, Tanishita K
    • Journal Title

      JSME International Journal, Ser.C 47・4

      Pages: 977-984

    • NAID

      110004820826

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Simultaneous imaging of initiator/effector caspase activity and mitochondrial membrane potential during cell death in living HeLa cells.2004

    • Author(s)
      Kawai H, Suzuki T, Kobayashi T, Mizuguchi H, Kawanishi T ら
    • Journal Title

      Biochemistry Biophysical Acta 1693

      Pages: 101-110

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Red blood cell velocity and oxygen tension measurement in cerebral microvessels by double-wavelength photoexcitation.2004

    • Author(s)
      Tsukada K., Sekizuka E., Oshio C., Tsujioka K., Minamitani H.
    • Journal Title

      J.Appl.Physiol. 96

      Pages: 1561-1568

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Photonic imaging analysis of blood flow and oxygentension in organ microcirculation.2004

    • Author(s)
      Minamitani H, Tsukada K
    • Journal Title

      Journal of Clinical and Experimental Medicine 210(3)(in Japanese)

      Pages: 200-204

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relationship between microtubule network structure and intracellular transport in cultured endothelial cells affected by shear stress.2004

    • Author(s)
      Kudo S, Ikezawa K, Ikeda M, Tanishita K
    • Journal Title

      JSMEIntJ, Ser C 47(4)

      Pages: 977-984

    • NAID

      110004820826

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Simultaneous imaging of initiator / effector caspase activity and mitochondrial membrane potential during cell death in living HeLa cells.2004

    • Author(s)
      Kawai H, Suzuki T, Kobayashi T, Mizuguchi H, Hayakawa T, Kawanishi T
    • Journal Title

      Biochim. Biophys. Acta 1693

      Pages: 101-110

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Red blood cell velocity and oxygen tension measurement in cerebral microvessels by double-wavelength photoexcitation2004

    • Author(s)
      Tsukada K., Minamitani H., et al.
    • Journal Title

      Journal of Applied Physiology 96

      Pages: 1561-1568

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 臓器血流と酸素代謝の光・イメージング解析2004

    • Author(s)
      南谷晴之, 塚田孝祐
    • Journal Title

      医学のあゆみ 210・3

      Pages: 200-204

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Blood flow dynamics and intravascular oxygen tension of tumor microvessels in photodynamic therapy2004

    • Author(s)
      Shibuya N., Minamitani H., et al.
    • Journal Title

      Microcirculation Annual 20

      Pages: 87-88

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Color imaging of platelets and leukocytes labeled with different fruorescent material in brain pial vessels of C57BL/6 mouse2004

    • Author(s)
      Taro S., Minamitani H., et al.
    • Journal Title

      Microcirculation Annual 20

      Pages: 99-100

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 腎臓血管傷害のイメージング2004

    • Author(s)
      Nagao T., Minamitani H., et al.
    • Journal Title

      Pharma medica 22・5

      Pages: 185-189

    • Related Report
      2004 Annual Research Report
  • [Book] Organ Microcirculation - A Gateway to Diagnostic and Therapeutic Interventions-, Role of Oxidative Stress in Interaction between Endothelial Cells and Platelets in Diabetes2005

    • Author(s)
      Y.Hirose, E.Sekizuka, H.Minamitanil
    • Total Pages
      297
    • Publisher
      Springer
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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