| Project/Area Number |
16300165
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo University of Science (2006)
National Institute for Materials Science (2004-2005) |
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Principal Investigator |
OTSUKA Hidenori Tokyo University of Science, Department of Applied Chemistry, Associate Professor, 理学部第一部応用化学科, 准教授 (00344193)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAHARA Yuji National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (20360399)
TANIGUCHI Akiyoshi National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (70256759)
KOBAYASHI Hisatoshi National Institute for Materials Science, Biomaterials Center, 生体材料センター, グループリーダー (90354266)
丸山 純夫 独立行政法人物質・材料研究機構, 生体材料研究センター・研究員
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2006: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2005: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2004: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | Biointerface / Microfabrication / Patterning / Spheroid / Cell Array / Hepatocyte / Photolithography / 軟骨 / パターニング / 微小組織エレメント |
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| Research Abstract |
We report here for the first time a simple and effective way to form a two-dimensional micro-array of ten thousand (100x100) hepatocyte hetero-spheroids. The hepatocyte hetero-spheroids were underlain with endothelial cells in an area of 20x20.mm, by using micro-fabricated glass substrates, coated with poly (ethylene glycol) (PEG) brushes. Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver specific function, allowing us to obtain hepatocyte spheroids with a diameter of 100-μm, functioning as a miniaturized liver to secret albumin for at least three weeks. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response. This system can be used as an alternative to animal experiments in a variety of situations in which the reliance on animals would prove impractical. Furthermore, the isolated spheroids from the array may have potential utility in constructing a tissue engineered liver by seeding spheroids into three-dimensional scaffolds.
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