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Gene delivery application of the polymer assembly interconnected by hydrogen bonding prepared by using ultra-high pressure

Research Project

Project/Area Number 16300167
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KISHIDA Akio  Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Professor (60224929)

Co-Investigator(Kenkyū-buntansha) FURUZONO Tsutomu  National Cardiovascular Center Research Institute, Department of Biomedical Engineering, Chief (30332406)
YAMAOKA Tetsuji  National Cardiovascular Center Research Institute, Department of Biomedical Engineering, Director (50243126)
KIMURA Tsuyoshi  Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Assoc Professor (10393216)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2006: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordshydrogen bond / high pressure / gene delivery / non-viral vector / inorganic particle / polymer / 遺伝子ベクター / ポリビニルアルコール / DNA
Research Abstract

The development of the complex for new gene delivery by the assembling molecule technology that utilizes the ultra high pressure was examined. This year, we examined following themes: 1) Examination of assembling DNA with polymers other than polyvinyl alcohol, 2) Application of inorganic micro particle that has hydrogen bond. The assembly with DNA was examined by using the polysaccharide including dextran, poly (acrylamide), poly (acrylic acid), poly (ethyleneglycol), and low molecular weight poly (hydroxymethylmethacrylate) as hydrogen bond polymers. Only the combination of dextran with poly (ethyleneglycol) gave molecular assembly using ultra-high pressure process. The assembly with plasmid DNA was examined about these two kinds of polymers. It was clarified that the formation of assembly was greatly influenced by the state of the solution. The compound was obstructed about the one that carbon dioxide in air dissolved to the distilled water once. In case of using the polymer to which the hydrogen bonds are weaker than poly (vinyl alcohol), it became clear that the experimental condition should be set more strictly. The assembling DNA with the hydroxyl group on the surface of the inorganic micro particle was examined. The hydroxyapatite nanocrystal that kept distributing in solution and had the hydroxyl groups on its surface was prepared, and the assembling with nanocrystals and DNA was examined. The compound of DNA stabilized, and was able to conduct the experiment on the gene delivery into the cell. The incorporation of plasmid DNA was high, however, the amount of appearance of the code protein was low. These results showed that a new mechanism had to be considered about the transportation to the nucleus in the cell and the examination of the condition of DNA assembling with other materials. As the candidate material, low molecular weight peptide will be examined because of that the structure of the peptide seemed not to be affected by the ultra-high pressure.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (30 results)

All 2008 2007 2006 2004

All Journal Article (14 results) Presentation (12 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] ressure-induced molecular assembly of hydrogen-bonded polymers2008

    • Author(s)
      Shingo Mutsuo
    • Journal Title

      J. Polym. Sci : Part B : Polym Phys 46

      Pages: 743-750

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pressure-induced molecular assembly of hydrogen-bonded polymers.2008

    • Author(s)
      Shingo Mutsuo
    • Journal Title

      J. Polym. Sci : Part B : Polym. Phys. 46

      Pages: 743-750

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation of poly (vinyl alcohol)/DNA hydrogels via hydrogen bonds formed on ultra-high pressurization and controlled release of DNA from the hydrogels for gene delivery2007

    • Author(s)
      Tsuyoshi Kimura
    • Journal Title

      Journal of Artificial Organs 10

      Pages: 104-108

    • NAID

      10026290009

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of compacted plasmid DNA by high pressurization2007

    • Author(s)
      Tsuyoshi Kimura
    • Journal Title

      Nucleic Acids Symposium Series No51

      Pages: 343-344

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation and characterization of cross-linked collagen-phospholipid polymer hybrid gels2007

    • Author(s)
      Kwangwoo Nam
    • Journal Title

      Biomaterials 28

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation and characterization of cross-linked collagen-phospholipid polymer hybrid gels.2007

    • Author(s)
      Kwangwoo Nam
    • Journal Title

      Biomaterials 28

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation of poly(vinyl alcohol)/DNA hydrogels via hydrogen bonds formed on ultra-high pressurization and controlled release of DNA from the hvdrogels for gene delivery.2007

    • Author(s)
      Tsuyoshi Kimura
    • Journal Title

      J Artif Organs 10

      Pages: 104-108

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of compacted plasmid DNA by high pressurization.2007

    • Author(s)
      Tsuyoshi Kimura
    • Journal Title

      Nucleic Acids Symposium Series No. 51

      Pages: 343-344

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation and characterization of cross-linked collagen-phospholipid polymer hybrid gels2007

    • Author(s)
      K.Nam., et al.
    • Journal Title

      Biomaterials 28

      Pages: 1-8

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Preparation of PVA/DNA hydrogels via hydrogen bonds with ultra high pressurization and controlled release of DNA from the hydrogels for gene delivery2007

    • Author(s)
      T.Kimura, et al.
    • Journal Title

      Journal of Artificial Organs 10(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene Transfection Using Inorganic Particle/PVA/DNA Complexes Prepared by Ultra High Pressure Technology2006

    • Author(s)
      Tsuyoshi Kimura
    • Journal Title

      Molecular Therapy 13

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gene Transfection Using Inorganic Particle/PVA/DNA Complexes Prepared by Ultra High Pressure Technology.2006

    • Author(s)
      Tsuyoshi Kimura
    • Journal Title

      Molecular Therapy 13

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gene transfection using inorganic particle/PVA/DNA complexes prepared by ultra high pressure technology2006

    • Author(s)
      T.Kimura, et al.
    • Journal Title

      Molecular Therapy 13, Suppl 1

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Novel PVA-DNA nanoparticles prepared by ultra high pressure technology fo gene delivery.2004

    • Author(s)
      Tsuyoshi Kimura, Akira Okuno, Kozo Miyazaki, Tsutomu Furuzono, Yuichi Ohya, Taturou Ouchi, Shingo Mutsuo, Hidekazu Yoshizawa, Yoshiro Kitamura, Toshiya Fujisato, Akio Kishida
    • Journal Title

      Matererials Science and Engineering C 24

      Pages: 794-801

    • Related Report
      2004 Annual Research Report
  • [Presentation] Preparation and evaluation of PVA hydrogel Preparede by ultra-high pressurization.2007

    • Author(s)
      Tsuyosi Kimura
    • Organizer
      18th Polymeric gel meeting
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Analysis of strucuture and function of DNA compacted by pressurization and application for gene delivery2007

    • Author(s)
      Tsuyoshi Kimura
    • Organizer
      The 6th Congress of the Japanese Society for Regerative Medicine
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Gene delivery using multi-polymer composites prepared by ultra high pressure treatment2006

    • Author(s)
      Yoshiyuki Miura
    • Organizer
      The 5th Congress of the Japanese Society for Regenerative Medicine
    • Place of Presentation
      Okayama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Gene delivery to cell using inoroganic nanosphere/polymer composites prepared by ultra-high pressure.2006

    • Author(s)
      Tsuyoshi Kimura
    • Organizer
      6th Gene delivery society
    • Place of Presentation
      Fukuoka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Gene delivery using nano-HAP/PVA/DNA complex promoting endosomal escape.2006

    • Author(s)
      Youichi Nibe
    • Organizer
      The 55th Annual Meeting, Society for Polymer Science
    • Place of Presentation
      Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Preparation of new assembly by ultra-high pressure treatment of PEG/polysaccharide aqueous polymer two phase separation.2006

    • Author(s)
      Yoshiyuki Miura
    • Organizer
      The 55th Annual Meeting, Society for Polymer Science, Japan
    • Place of Presentation
      Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Preparation of nano-inorganic particle/ polymer/DNA compsites by ultra high pressure technology for genetransfection.2006

    • Author(s)
      Tsuyoshi Kimura
    • Organizer
      The 55th Annual Meeting, Society for Polymer Science, Japan
    • Place of Presentation
      Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Study for distribution in cells on gene delivery using inorganic nanoparticle/polymer hybrids prepared by ultra-high pressure.2006

    • Author(s)
      Tsuyoshi Kimura
    • Organizer
      The 22nd Congress of DDS Society
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Preparation and in vitro evaluation of inorganic/polymeric composite prepared by ultra-high pressure.2006

    • Author(s)
      Tsuyoshi Kimura
    • Organizer
      The 35th Medical Polymer Symposium
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Ultra high pressure technology for controlling the structure of DNA/RNA2006

    • Author(s)
      Tsuyoshi Kimura
    • Organizer
      55th Symposium on Macromolecules
    • Place of Presentation
      Toyama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Preparation of Hydrogen Bonding Polymer Structures Using Ultra High Pressure Technology as Drug Carrier.2006

    • Author(s)
      Yoshiyuki Miura
    • Organizer
      AICHE
    • Place of Presentation
      San Francisco
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Enhancement of gene delivery by adding inorganic salt to PVA/DNA gene vector prepared by ultra-high pressure.2006

    • Author(s)
      Tsuyoshi Kimura
    • Organizer
      28th Japanese Biomaterial Society, Annual Meeting
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 遺伝子導入におけるセラミック材料-リン酸カルシウムを中心に-、先端生物医学研究・医療のための遺伝子導入テクノロジー「ウイルスを用いない遺伝子導入法の材料, 技術, 方法論の新たな展開」、原島秀吉、田畑泰彦編2006

    • Author(s)
      木村剛
    • Total Pages
      4
    • Publisher
      メディカルドゥ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] ポバール会記録2004

    • Author(s)
      木村剛, 奥野暁, 大矢裕一, 大内辰郎, 六雄伸吾, 吉澤秀和, 古薗勉, 藤里俊哉, 岸田晶夫
    • Publisher
      ポバール会
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 機能性DNAの製造方法、形質転換体、及び疾患治療剤2007

    • Inventor(s)
      岸田晶夫, 木村剛, 南広祐, 藤里俊哉
    • Industrial Property Rights Holder
      東京医科歯科大学
    • Industrial Property Number
      2007-263704
    • Filing Date
      2007-10-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 遺伝子導入のための分子複合体2006

    • Inventor(s)
      岸田晶夫, 木村剛, 吉潭秀和
    • Industrial Property Rights Holder
      東京医科歯科大学
    • Patent Publication Number
      2006-230343
    • Filing Date
      2006-09-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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