| Project/Area Number |
16300174
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
ITOH Kouichi JMS, School of Medicine, Professor, 医学部, 教授 (60095007)
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| Co-Investigator(Kenkyū-buntansha) |
OOKAWARA Shigeo JMS, School of Medicine, Professor, 医学部, 教授 (60049088)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Macrophage / Blood Brain Barrier / Hepatocyte / Kupffer cell / Platelet activation / Safety / Ultrasound / Ultrasound contrast agent / スカベンジャー細胞 / 光学顕微鏡 / 電子顕微鏡 |
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| Research Abstract |
We studied the bio-effects of Ultrasound and contrast agents to hepatic cells. The percentage of CD62P-expressing platelets increased in both contrast USE groups, and the percentage of CD62P-expressing platelets was greater in Levovist group. We observed vacuolation and round deposits in the hepatocytes in both contrast-USE groups. Microbubbles were observed in the rat Kupffer cells and a few hepatocytes unexpectedly in the DD-723 group, but were found in neither the Kupffer cells nor the hepatocytes in the Levovist group.
The bio-effects of the ultrasound and contrast agents to the Macrophage, which is named of Fluorescent Granular Perithelial Cells, around brain arteries can be seen for as increase and damage of Lysosome and edematic changes.
Conclusion the contrast agents, Levovist, DD-723 and Optizone, produced platelet activation and structural change in the rat hepatic cells and the macrophages around brain arteries, but only the microbubbles of DD-723 were taken up by the Kupffer cells and a few hepatocytes. In the brain, Ultrasound and contrast agents give the damage of Macrophage which is a part of Blood-Brain Barrier.
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