| Project/Area Number |
16300175
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
TANIGUCHI Nobuyuki JMS, School of Medicine, Associate Professor, 医学部, 助教授 (10245053)
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| Co-Investigator(Kenkyū-buntansha) |
FUJII Yasutomo JMS, School of Medicine, Assistant Professor, 医学部, 講師 (00337338)
KAWANO Mikihiko JMS, School of Medicine, Professor, 医学部, 教授 (40254940)
ITOH Kouichi JMS, School of Medicine, Professor, 医学部, 教授 (60095007)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2004: ¥7,300,000 (Direct Cost: ¥7,300,000)
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| Keywords | Therapeutic ultrasound / Obesity / Human adipocyte / In vitro / ELISA / Adiponectin |
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| Research Abstract |
To clarify the effect of the repeated sonication on the adiponectin secretion by adipocytes obtained from obese subjects. Using 1-MHz continuous-wave ultrasound at an intensity of 0.50 or 2.1W/cm^2, we sonicated culture flasks of subcutaneous adipocytes obtained from obese human subjects, in a series of 3 sessions of US stimulation applied for a daily total of 15min. For the measurement of adiponectin secretion, 50μl of the culture medium was collected from each flask every 24h after the 1st stimulation. Quantification of adiponectin protein levels in cell culture supernatants was performed with a commercially available ELISA kit recommended by the manufacturer. The adiponectin concentrations in the culture medium of the US stimulation groups rose significantly (p<0.05). Repeated US stimulation may accelerate adiponectin secretion in obese human adipocytes.
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