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Effect of US stimulation on adiponectin secretion by adipocytes of human subjects

Research Project

Project/Area Number 16300175
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical systems
Research InstitutionJICHI MEDICAL SCHOOL

Principal Investigator

TANIGUCHI Nobuyuki  JMS, School of Medicine, Associate Professor, 医学部, 助教授 (10245053)

Co-Investigator(Kenkyū-buntansha) FUJII Yasutomo  JMS, School of Medicine, Assistant Professor, 医学部, 講師 (00337338)
KAWANO Mikihiko  JMS, School of Medicine, Professor, 医学部, 教授 (40254940)
ITOH Kouichi  JMS, School of Medicine, Professor, 医学部, 教授 (60095007)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2004: ¥7,300,000 (Direct Cost: ¥7,300,000)
KeywordsTherapeutic ultrasound / Obesity / Human adipocyte / In vitro / ELISA / Adiponectin
Research Abstract

To clarify the effect of the repeated sonication on the adiponectin secretion by adipocytes obtained from obese subjects. Using 1-MHz continuous-wave ultrasound at an intensity of 0.50 or 2.1W/cm^2, we sonicated culture flasks of subcutaneous adipocytes obtained from obese human subjects, in a series of 3 sessions of US stimulation applied for a daily total of 15min. For the measurement of adiponectin secretion, 50μl of the culture medium was collected from each flask every 24h after the 1st stimulation. Quantification of adiponectin protein levels in cell culture supernatants was performed with a commercially available ELISA kit recommended by the manufacturer. The adiponectin concentrations in the culture medium of the US stimulation groups rose significantly (p<0.05). Repeated US stimulation may accelerate adiponectin secretion in obese human adipocytes.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (4 results)

All 2006 2005

All Journal Article (4 results)

  • [Journal Article] Effect of repeated US stimulation on adiponectin secretion by adipocytes of obese human subjects2006

    • Author(s)
      Fujii Y, Taniguchi N, Satoh M, Irie T, Itoh K.
    • Journal Title

      The American Institute of Physics Conference Proceedings for 5th International Symposium on Therapeutic Ultrasound (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 超音波照射システムの開発2005

    • Author(s)
      佐藤正和, 藤井康友, 谷口信行, 安齋二郎, 入江喬介, 伊東紘一
    • Journal Title

      日本超音波医学会基礎技術研究科資料 2005-2

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of repeated US stimulation on adiponectin secretion by adipocytes of obese human subjects2005

    • Author(s)
      Fujii Y, Taniguchi N, Satou M, Irie T, Itoh K
    • Journal Title

      The American Institute of Physics Conference Proceedings for 5th International Symposium on Therapeutic Ultrasound (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 超音波照射システムの開発2005

    • Author(s)
      佐藤正和, 藤井康友, 谷口信行, 安齋二郎, 入江喬介, 伊東紘一
    • Journal Title

      日本超音波医学会基礎技術研究会資料 2005-2

      Pages: 1-6

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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