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Regulatory role by orexin on the development of anti-gravity muscle and exercise-related functions

Research Project

Project/Area Number 16300204
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Sports science
Research InstitutionUniversity of Tsukuba

Principal Investigator

SOYA Hideaki  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Associate professor, 大学院人間総合科学研究科, 助教授 (50221346)

Co-Investigator(Kenkyū-buntansha) SAKURAI Takeshi  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Associate professor, 大学院人間総合科学研究科, 助教授 (60251055)
KIZUKA Tomohiro  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Associate professor, 大学院人間総合科学研究科, 助教授 (30323281)
佐久間 邦弘  京都府立医科大学, 医学研究科, 講師 (60291176)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2005: ¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 2004: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsorexin / hindlimb muscle / alpha-motorneuron / defense Response / emotional stress / ACTH / orexin 1 receptor / orexin 2 receptor / ナルコレプシー / 抗重力筋 / ヒラメ筋 / プレプロオレキシンノックアウトマウス
Research Abstract

Orexins (OXs) have been known as a hypothalamic regulating factors in the defense response, so called "fight or flight" response, which is characterized by a concomitant activation in arousal, HPA-axis, sympathetic nerve system and alpha-motoneurons (α MN) facilitation. These physiological reactions are orchestrated to involved in the regulation of physical activity in "fight or flight" response. We hypothesized that OXs would simultaneously be involved in the αMN facilitation and HPA-axis activation. To address this, we examined whether OX-1R (type-1-receptor) expresses on αMN, connecting to hindlimb soleus and extensor digitorum longus (EDL) muscle, respectively. As a result, almost all αMN to their respective muscles had OX-1R. To identify whether OXs were involved in the αMN-facilitation via OX-1R in lumbar, we examined the escape swimming response to stress provoked by immersing rats into water after pretreatment of OX-1R antagonist administration. The inhibition of OXs action via … More OX-1R on αMN decreased the escape swimming activity. Under the same stress, neurons in PVN and LHA were activated. ACTH release which promoted by water immersion stress was suppressed by pretreatment of OX-2R (type-2-receptor) antagonist administration.
Plasma concentrations of OXs were higher in neonates and infant during decelopment, and OX-receptors express on skeletal muscles and αMNs. It leads us hypothesized that OXs would mediate growth and development of skeletal muscle through both humoral and neuronal pathways. First, we examined hind limb muscular weight between prepro-OX knock-out (KO) mice and littermate wild-type (Wt) mice (10-50 weeks-old), and found that both plantaris and EDL muscles in KO mice atrophied significantly. In addition, we investigated the effects of OXs on the proliferation and defferentation of myoblasts in vitro culture system, and found that OXs accelerated the proliferation of myoblasts.
We found that OXs plays dual roles for facilitating αMN and the HPA-axis through their specific receptors. Again, OXs play a potential role to promote myoblasts' proliferation in vitro. These results underscore orexin's role in muscular development and exercise-related functions, leading us to examine the role of orexin on some response and adaptation of body to exercise. (349) Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (9 results)

All 2007 2006 2004 Other

All Journal Article (9 results)

  • [Journal Article] Inhibitory effects of an orexin-2 receptor antagonist on orexin A-and stress-induced ACTH responses in conscious rats.2007

    • Author(s)
      Chang Hyukki
    • Journal Title

      Neuroscience Research 57

      Pages: 462-466

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effects of an orexin-2 receptor 'antagonist on orexin A-and stress-induced ACTH responses in conscious rats.2007

    • Author(s)
      Hyukki Chang
    • Journal Title

      Neuroscience Research 57

      Pages: 462-466

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 遊泳運動ストレスによる脳内オレキシン神経の活性化2006

    • Author(s)
      張赫起
    • Journal Title

      日本運動生理学雑誌 13

      Pages: 39-46

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Swimming stress activates orexin-containing neuron in the hypothalamus in rat brain.2006

    • Author(s)
      Hyukki Chang
    • Journal Title

      Journal of exercise and sports physiology 13

      Pages: 39-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 走運動時のストレス反応とオレキシン神経の活性化2004

    • Author(s)
      征矢英昭
    • Journal Title

      自律神経学会誌 41

      Pages: 116-121

    • NAID

      10013353867

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Running induces stress response and orexin-containing neurons.2004

    • Author(s)
      Hideaki Soya
    • Journal Title

      The Autonomic nervous system 41

      Pages: 7116-121

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 走運動時のストレス反応とオレキシン神経の活性化2004

    • Author(s)
      征矢英昭, 齋藤剛, 張赫起, 楯岡卓
    • Journal Title

      自律神経 41巻2号

      Pages: 116-121

    • NAID

      10013353867

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 姿勢と脳「-かみ合わせとの関連性-」

    • Author(s)
      征矢英昭
    • Journal Title

      咬合学会誌 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The brain involved in keeping posture -the relation between masseter muscle and muscle keeping posture-

    • Author(s)
      Hideaki Soya
    • Journal Title

      Japan Academy of Occlusion (In printing)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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