| Project/Area Number |
16310101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
SEKI Minoru Osaka Prefecture University, Grad.School of Eng., Professor, 工学研究科, 教授 (80206622)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Masahiro Osaka Prefecture University, Grad.School of Eng., Assistant Professor, 工学研究科, 講師 (40264808)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2006: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2005: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2004: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | Microfluidic Device / Micro / Nano Dispenser / Micro-reactor Array / Microfluidics / Polydimethylsiloxane / Accumulation of Micro-particles / Contact Angle / Local Surface Modification / シランカップリング |
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| Research Abstract |
The purpose of this research is to develop a micro-dispenser that enables the quantitative handling with multiple ultra-low volume liquid in a simple procedure and to construct a micro/nano reactor array by multiplying the developed dispensers with a volume of nano-liter to pico-liter. The main results are as follows :
1.Qualification of Developed Micro Liquid Dispenser
In this research, we have proposed a novel dispensing principle for a low-volume liquid, that is, a simple micro-channel network without any mechanical elements. This microchannel structure has both the narrow sections that function as a fluid valve and the multiple branching points that can quantitatively meter the small liquid volume. The former valve-like structure is generally called as a "passive valve", that requires the narrow and hard-wet region, i.e., the contact angle of the handling liquid for the channel surface is more than 90 degree. It has revealed that our newly proposed channel structure has a clear valve
… More
function either for a wettable or hard to wet liquid, even though that depends on the fabrication accuracy.
2.Local Surface Modification for the Dispenser Improvement
The surface modification of the channel inner wall improves the valve performance, especially for the narrow region of the dispenser. For this purpose, various silane coupling reagents were effective for PDMS microchannel modification. We have also proposed a novel local surface modification method utilizing the accumulation of small polymer particles at the specific area on a microchannel inner wall and the melting of the particles after the accumulation.
3.Construction of Micro/Nano Reactor Array with Multiple Micro-Dispensers
We have also developed the various types of micro/nano reactor arrays by integrating multiple micro-dispensers, that are, a 2D-microreactor array that enables the three droplets mixing at each chamber for the multiple drug resistance screening, a micro-gel compartment array using Ca-alginate for cell screening, and a chamber array that easily prepares the concentration gradient by exchanging two kinds of liquids. Less
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