Comparative genomics of gene expression mechanisms of parasitic protozoa using full-length cDNA

• Project/Area Number: 16310132
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 基礎ゲノム科学
• Research Institution: The University of Tokyo
• Principal Investigator: WATANABE Junichi The University of Tokyo, Institute of Medical Science, Basic Medicine, Assistant Professor (20201189)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Yutaka The University of Tokyo, Graduate School of Frontier Sciences, Medical Genomics, Associate Professor (40323646) ; YAMASHITA Riu The University of Tokyo, Institute of Medical Science, Human Genome Center, Assistant Professor (10401259) ; SUGIMOTO Chihiro Hokkaido University, Center for Zoonosis Control, Professor (90231373)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥15,600,000); Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2005: ¥6,100,000 (Direct Cost: ¥6,100,000); Fiscal Year 2004: ¥6,500,000 (Direct Cost: ¥6,500,000)
• Keywords: parasitic protozoa / malaria / toxoplasma / comparative genomics / gene expression / expression mechanism / 全長cDNA / 遺伝子発現制御 / 全長cDNAライブラリ / マラリア原虫 / トキソプラズマ原虫

Research Abstract

Parasitic protozoa are the simplest eukaryotes of which genomes have been sequenced because of their medical importance. As the attempt to study gene expression mechanism of eukaryotes, we have produced full-length cDNA libraries using the oligo-capping method which we have originated from various parasites including tertian malaria parasite, Plasmodium vivax, murine malaria parasite, P. berghei, Toxoplasma gondii, Cryptosporidium parvum, Theileria parva, T. orientalis, and Eimeria tenella, and determined 5'-end-one-pass sequences from the random clones. These sequences have been mapped onto the respective genome sequences and presented in the genome browsers. For comparative genomic analysis, a full-length cDNA library has been produced from flatworm, Echinococcus multilocularis. The results have been compiled as a database, Full-Malaria and published in internet at http : //fullmal.ims.u-tokyo.ac.jp. Furthermore, comparative genomics database, Comparasite, (http : //comparasite.hgc.ip) has been produced. These provide basic information for comparative genome analysis to elucidate the exact mechanisms of gene expression. In addition, using the original method to screen novel malaria DNA vaccine candidates, promising murine malaria vaccine candidate clones have been selected. This method should be applicable to other eukaryotic pathogens.

Research Products

• [Journal Article] Comparasite:a database for comparative study of transcriptomes ofparasites defined by full-length cDNAs (2007)
  • Author(s): Junichi Watanabe
  • Journal Title: Nucleic Acids Research 35
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comparasite : a database for comparative study of transcriptomes of parasites defined by full-length cDNAs (2007)
  • Author(s): Junichi Watanabe
  • Journal Title: Nucleic Acids Research 35
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Malaria Research Trends (2007)
  • Author(s): Josef Tuda
  • Journal Title: Nova Publishers Pages: 1-2
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparasite : a database for comparative study of transcriptomes of parasties defined by full-length cDNAs. (2007)
  • Author(s): Junichi Watanabe, Hiroyuki Wakaguri, Masahide Sasaki, Yutaka Suzuki, Sumio Sugano.
  • Journal Title: Nucleic Acids Research 35(Database issue)
• [Journal Article] Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii (2006)
  • Author(s): Khan A
  • Journal Title: Genome Res. 16(9) Pages: 1119-1125
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii. (2006)
  • Author(s): Khan A, Bohme U, Kelly KA, Adlem E, Brooks K, Simmonds M, Mungall K, Quail MA, Arrowsith C, Chillingworth T, Churcher C, Harris D, Collins M, Fosker N, Fraser A, Hance Z, Jagels K, Moule S, Murphy L, O'Nel S, Rajandream MA, Saunders D, Seeger K, Whitehead S, Mayr T, Xuan X, Watanabe J, Suzuki Y, Wakaguri H, Mackey AJ, Roos DS, Hall N, Berriman M, Barrell B, Sibley LD, Ajioka JW.
  • Journal Title: Genome Res. 16(9) Pages: 1119-25
• [Journal Article] A novel method for development of malaria vaccines using full-length cDNA Iibraries (2005)
  • Author(s): Akiko Shibui
  • Journal Title: Vaccine 23 Pages: 4359-4366
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A novel method for development of malaria vaccines using full-length cDNA libraries (2005)
  • Author(s): Akiko Shibui
  • Journal Title: Vaccine 23 Pages: 4359-4366
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel method for development of malaria vaccines using full-length cDNA libraries (2005)
  • Author(s): Akiko Shibui, Takashi Shiibashi, Sadao Nogami, Sumio Sugano, Junichi Watanabe
  • Journal Title: Vaccine 23 Pages: 4359-4366
• [Journal Article] Cloning and characterization of cDNA encoding a prohibitin-like protein from Theileria orientalis. (2005)
  • Author(s): He W, Ohashi K, Sugimoto C, Tsuji M, Onuma M.
  • Journal Title: Jpn J Vet Res. 53 Pages: 27-35
  • NAID: 110004665692
• [Journal Article] Theileria orientalis : cloning a cDNA encoding a protein similar to thiol protease with haemoglobin-binding activity. (2005)
  • Author(s): He W, Ohashi K, Sugimoto C, Onuma M.
  • Journal Title: Exp Parasitol. 111 Pages: 143-153
• [Journal Article] Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs. (2005)
  • Author(s): Thekisoe OM, Inoue N, Kuboki N, Tuntasuvan D, Bunnoy W, Borisutsuwan S, Igarashi I, Sugimoto C
  • Journal Title: Vet Parasitol. 130 Pages: 327-330
• [Journal Article] Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli. (2005)
  • Author(s): Gaturaga I, Chahan B, Xuan X, Huang X, Liao M, Fukumoto S, Hirata H, Nishikawa Y, Takashima Y, Suzuki H, Fujisaki K, Sugimoto C.
  • Journal Title: J Parasitol. 91 Pages: 191-192
• [Journal Article] Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated. (2005)
  • Author(s): Baticados WN, Witola WH, Inoue N, Kim JY, Kuboki N, Xuan X, Yokoyama N, Sugimoto C.
  • Journal Title: J Vet Med Sci. 67 Pages: 157-164
  • NAID: 110003963695
• [Journal Article] Full-length cDNAs from Plasmodium and Toxoplasma (2005)
  • Author(s): Watanabe J, Xuan X, Suzuki Y, Sasaki M, Wakaguri H, Sugimoto C, Sugano S
  • Journal Title: Nucleic Acids Research (http://www3.oup.co.uk/nar/database/summary/72) 31
• [Journal Article] A novel method for development of malaria vaccines using full-length cDNA libraries (2005)
  • Author(s): Akiko Shibui, Takashi Shiibashi, Sadao Nogami, Sumio Sugano, Junichi Watanabe
  • Journal Title: Vaccine (発表予定)
• [Journal Article] Lethal malaria in cytosolic phospholipase A_2-and phospholipase _2IIA-deficient mice (2004)
  • Author(s): Shumpei Ishikawa
  • Journal Title: Am.J.Trop.Med.Hyg 70(6) Pages: 645-650
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Lethal malaria in cytosolic phospholipase A2-and phospholipase A2IIA-deficient mice (2004)
  • Author(s): Shumpei Ishikawa
  • Journal Title: Am. J. Trop. Med. Hyg. 70(6) Pages: 645-650
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] LETHAL MALARIA IN CYTOSOLIC PHOSPHOLIPASE A2-AND PHOSPHOLIPASE AIIA-DEFICIENT MICE (2004)
  • Author(s): Ishikawa S, Uozumi N, Shiibashi T, Izumi T, Fukayama M, Shimizu T, Watanabe J, Nogami S
  • Journal Title: Amer.J.Trop.Med.Hyg. 70 Pages: 645-650
• [Book] Malaria Research Trends (2007)
  • Author(s): Josef Tuda
  • Publisher: Nova Publishers
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Impact of full-length cDNA library to genome sequencing of malaria parasites, Plasmodium Species in Recent Devel. Nucleic Acids Res. (2004)
  • Author(s): Junichi Watanabe, Yutaka Suzuki, Hiroyuki Wakaguri Masahide Sasaki, Sumio Sugano
  • Publisher: Transworld Research Network