| Project/Area Number |
16310134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | University of Miyazaki |
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Principal Investigator |
NAKAYAMA Tatsuo University of Miyazaki, Frontier Science Research Center, Professor, フロンティア科学実験総合センター, 教授 (60031712)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAMI Yasunari University of Miyazaki, Department of Medical Sciences, Associate Professor, 医学部, 助教授 (80236356)
KIKUCHI Hidehiko University of Miyazaki, Frontier Science Research Center, Assistant Professor, フロンティア科学実験総合センター, 助手 (10301384)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2006: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Histone modifying enzyme / Gene targeting / DT40 / Cell cycle / Immunoglobulin / Histone chaperone / Apoptosis / Gene expression |
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| Research Abstract |
1. WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with CAF-1p48 subunit and HDAC2 i a vitro and in vivo.
2. GCN5 is a supervisor in all-inclusive control of vertebrate cell cycle progression via transcription regulation of various cell cycle-related genes.
3. N-terminal and C-terminal halves of HIRA exhibit different roles in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth.
4. HAT1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.
5. ASF1 is essential for viability and chromatin assembly during DNA replication in vertebrate cells.
6. CAF-1-mediated rapid nucleosome assembly is essential for DNA replication and cell division in vertebrate cells.
7. HDAC2 is necessary for controls of IgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions.
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