Mechanisms for all-inclusive controls of cell cycle progression, and IgM H and L-chain gene expressions by GCN5 and HDAC2

• Project/Area Number: 16310134
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 基礎ゲノム科学
• Research Institution: University of Miyazaki
• Principal Investigator: NAKAYAMA Tatsuo University of Miyazaki, Frontier Science Research Center, Professor, フロンティア科学実験総合センター, 教授 (60031712)
• Co-Investigator(Kenkyū-buntansha): TAKAMI Yasunari University of Miyazaki, Department of Medical Sciences, Associate Professor, 医学部, 助教授 (80236356) ; KIKUCHI Hidehiko University of Miyazaki, Frontier Science Research Center, Assistant Professor, フロンティア科学実験総合センター, 助手 (10301384)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥15,700,000 (Direct Cost: ¥15,700,000); Fiscal Year 2006: ¥4,600,000 (Direct Cost: ¥4,600,000); Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000); Fiscal Year 2004: ¥6,500,000 (Direct Cost: ¥6,500,000)
• Keywords: Histone modifying enzyme / Gene targeting / DT40 / Cell cycle / Immunoglobulin / Histone chaperone / Apoptosis / Gene expression

Research Abstract

1. WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with CAF-1p48 subunit and HDAC2 i a vitro and in vivo.
2. GCN5 is a supervisor in all-inclusive control of vertebrate cell cycle progression via transcription regulation of various cell cycle-related genes.
3. N-terminal and C-terminal halves of HIRA exhibit different roles in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth.
4. HAT1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.
5. ASF1 is essential for viability and chromatin assembly during DNA replication in vertebrate cells.
6. CAF-1-mediated rapid nucleosome assembly is essential for DNA replication and cell division in vertebrate cells.
7. HDAC2 is necessary for controls of IgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions.

Research Products

• [Journal Article] Essential role of CAF-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells (2007)
  • Author(s): Takami, Y., Ono, T., Fukagawa, T., Shibahara, K., Nakayama, T.
  • Journal Title: Mol. Biol. Cell 18 Pages: 129-141
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Collaborative roles of rH2AX and the Rad51 paralog Xrcc3 in homologous recombinational repair (2007)
  • Author(s): Sonoda, E., Zhao, G.-Y., Kohzaki, M., Dhar, P.K., Kikuchi, K., Redon, C., Pilch, D.R., Bonner, W.M., Nakano, A., Watanabe, M., Nakayama, T., Takeda, S., Takami, Y.
  • Journal Title: DNA Repair 6 Pages: 280-292
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cleavage and cytoplasmic relocalisation of histone deacetylase 3 is important for apoptosis (2007)
  • Author(s): Escaffit, F., Vaute, O., Chevillard-Briet, M., Segui, B., Takami, Y., Nakayama, T., Trouche, D.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 554-567
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] HDAC2 controls IgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions (2007)
  • Author(s): Nakayama, M., Suzuki, H., Yamamoto-Nagamatsu, N., Barman, H.K., Kikuchi, H., Takami, Y., Toyonaga, K, Yamashita, K., Nakayama, T.
  • Journal Title: Genes Cells 12 Pages: 359-373
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Essential role of CAF-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells. (2007)
  • Author(s): Takami, Y, Ono, T., Fukagawa, T., Shibahara, K., Nakayama, T.
  • Journal Title: Mol. Biol. Cell 18 Pages: 129-141
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Collaborative roles of rH2AX and the Rad51 paralog Xrcc3 in homologous recombinational repair. (2007)
  • Author(s): Sonoda, E., Zhao, G-Y, Kohzaki, M., Dhar, P.K., Kikuchi, K., Redon, C., Pilch, D.R., Bonner, W.M., Nakano, A., Watanabe, M., Nakayama, T., Takeda, S., Takami, Y.
  • Journal Title: DNA Repair 6 Pages: 280-292
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cleavage and cytoplasmic relocalisation of histone deacetylase 3 is important for apoptosis. (2007)
  • Author(s): Escaffit, F., Vaute, O., Chevillard-Briet, M., Segui, B., Takami, Y., Nakayama, T., Trouche, D.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 554-567
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] HDAC2 controls IgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions. (2007)
  • Author(s): Nakayama, M., Suzuki, H., Yamamoto-Nagamatsu, N., Barman, H.K., Kikuchi, H., Takami, Y., Toyonaga, K., Yamashita, K., Nakayama, T.
  • Journal Title: Genes Cells 12 Pages: 359-373
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Essential role of CAF-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells (2007)
  • Author(s): Takami, Y., Ono, T., Fukagawa, T., Shibahara, K., Nakayama, T.
  • Journal Title: Mol.Biol.Cell 18 Pages: 129-141
• [Journal Article] Cleavage and cytoplasmic relocalisation of histone deacetylase 3 is important for apoptosis (2007)
  • Author(s): Escaffit, F., Vaute, O., Chevillard-Briet, M., Segui, B., Takami, Y., Nakayama, T., Trouche, D.
  • Journal Title: Mol.Cell.Biol. 27 Pages: 554-567
• [Journal Article] HDAC2 controls lgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions (2007)
  • Author(s): Nakayama, M., Suzuki, H., Yamamoto-Nagamatsu, N., Barman, H.K., Kikuchi, H., Takami, Y., Toyonaga, K., Yamashita, K., Nakayama, T.
  • Journal Title: Genes Cells 12 Pages: 359-373
• [Journal Article] Asfl is required for viability and chromatin assembly during DNA replication in vertebrate cells (2006)
  • Author(s): Sanematsu, F., Takami, Y., Barman, H.K., Fukagawa, T., Ono, T., Shibahara, K., Nakayama, T.
  • Journal Title: J. Biol. Chem. 281 Pages: 13817-13827
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells (2006)
  • Author(s): Barman, H.K., Takami, Y., Ono, T., Nishijima, H., Sanematsu, F., Shibahara, K., Nakayama, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 345 Pages: 1547-1557
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Reviews and Protocols in DT40 Research : Participation of histones, histone modifying enzymes and histone chaperones in vertebrate cell functions. (2006)
  • Author(s): Kikuchi, H., Barman, H.K., Nakayama, M., Takami, Y, Nakayama, T.
  • Journal Title: Springer-Verlag Pages: 225-243
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Asfl is required for viability and chromatin assembly during DNA replication in vertebrate cells. (2006)
  • Author(s): Sanematsu, F., Takami, Y., Barman, H.K., Fukagawa, T., Ono, T., Shibahara, K., Nakayama, T.
  • Journal Title: J. Biol. Chem. 281 Pages: 13817-13827
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells. (2006)
  • Author(s): Barman, H.K., Takami, Y., Ono, T., Nishijima, H., Sanematsu, F., Shibahara, K., Nakayama, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 345 Pages: 1547-1557
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells (2006)
  • Author(s): Sanematsu, F., Takami, Y., Barman, H.K., Fukagawa, T., Ono, T., Shibahara, K., Nakayama, T.
  • Journal Title: J.Biol.Chem. 281 Pages: 13817-13827
• [Journal Article] Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells (2006)
  • Author(s): Barman, H.K., Takami, Y., Ono, T., Nishijima, H., Sanematsu, F., Shibahara, K., Nakayama, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 345 Pages: 1547-1557
• [Journal Article] GCN5 : a supervisor in all-inclusive control of vertebrate cell cycle progression through transcription regulation of various cell cycle-related genes (2005)
  • Author(s): Kikuchi, H., Takami, Y., Nakayama, T.
  • Journal Title: Gene 347 Pages: 83-97
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of NAD-dependent deacetylases SIRT1 and SIRT2 in radiation and cisplatin-induced cell death in vertebrate cells (2005)
  • Author(s): Matsushita, N., Takami, Y., Kimura, M., Tachiiri, S., Ishiai, M., Nakayama, T., Takata, M.
  • Journal Title: Genes Cells 10 Pages: 321-332
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Different roles of N-terminal and C-terminal halves of HIRA in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth (2005)
  • Author(s): Ahmad, A., Kikuchi, H., Takami, Y., Nakayama, T.
  • Journal Title: J. Biol. Chem. 280 Pages: 32090-32100
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] a supervisor in all-inclusive control of vertebrate cell cycle progression through transcription regulation of various cell cycle-related genes. (2005)
  • Author(s): Kikuchi, H., Takami, Y., Nakayama, T.GCN5
  • Journal Title: Gene 347 Pages: 83-97
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of NAD-dependent deacetylases SIRT1 and SIRT2 in radiation and cisplatin -induced cell death in vertebrate cells. (2005)
  • Author(s): Matsushita, N., Takami, Y, Kimura, M., Tachiiri, S., Ishiai, M., Nakayama, T., Takata, M.
  • Journal Title: Genes Cells 10 Pages: 321-332
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Different roles of N-terminal and C-terminal halves of HIRA in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth. (2005)
  • Author(s): Ahmad, A., Kikuchi, H., Takami, Y., Nakayama, T.
  • Journal Title: J. Biol. Chem. 280 Pages: 32090-32100
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] GCN5 : a supervisor in all-inclusive control of vertebrate cell cycle progression through transcription regulation of various cell cycle-related genes. (2005)
  • Author(s): Kikuchi, H., Takami, Y., Nakayama, T.
  • Journal Title: Gene 347 Pages: 83-97
• [Journal Article] Role of NAD-dependent deacetylases SIRT1 and SIRT2 in radiation and cisplatin-induced cell death in vertebrate cells. (2005)
  • Author(s): Matsushita, N., Takami, Y., Kimura, M., Tachiiri, S., Ishiai, M., Nakayama, T.Takata, M.
  • Journal Title: Genes Cells 10 Pages: 321-332
• [Journal Article] Different roles of N-terminal and C-terminal halves of HIRA in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth. (2005)
  • Author(s): Ahmad, A., Kikuchi, H., Takami, Y., Nakayama, T.
  • Journal Title: J.Biol.Chem. 280 Pages: 32090-32100
• [Journal Article] GCN5:a supervisor in all-inclusive control of vertebrate cell cycle progression through transcription regulation of various cell cycle-related genes. (2005)
  • Author(s): Kikuchi, H., Takami, Y., Nakayama, T
  • Journal Title: Gene 347 Pages: 83-97
• [Journal Article] Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity (2004)
  • Author(s): Takechi, S., Yamaguchi, T., Nomura, H., Minematsu, T., Nakayama, T.
  • Journal Title: Mutation Research 560 Pages: 49-55
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Dicer is essential for formation of the heterochromatin structure in vertebrate cells (2004)
  • Author(s): Fukagawa, T., Nogami, M., Yoshikawa, M., Ikeno, M., Okazaki, T., Takami, Y., Nakayama, T., Oshimura, M.
  • Journal Title: nature Cell Biology 8 Pages: 784-791
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with the p48 subunit of chromatin assembly factor-1 and histone deacetylase-2 in vitro and in vivo (2004)
  • Author(s): Ahmad, A., Takami, Y., Nakayama, T.
  • Journal Title: Gene 342 Pages: 125-136
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand- cleaving activity. (2004)
  • Author(s): Takechi, S., Yamaguchi, T., Nomura, H., Minematsu, T., Nakayama, T.
  • Journal Title: Mutation Research 560 Pages: 49-55
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dicer is essential for formation of the heterochromatin structure in vertebrate cells. (2004)
  • Author(s): Fukagawa, T., Nogami, M., Yoshikawa, M., Ikeno, M., Okazaki, T., Takami, Y., Nakayama, T., Oshimura, M.
  • Journal Title: nature Cell Biology 8 Pages: 784-791
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with the p48 subunit of chromatin assembly factor-1 and histone deacetylase-2 in vitro and in vivo. (2004)
  • Author(s): Ahmad, A., Takami, Y., Nakayama, T.
  • Journal Title: Gene 342 Pages: 125-136
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity. (2004)
  • Author(s): Takechi, S., Yamaguchi, T., Nomura, H., Minematsu, T., Nakayama, T.
  • Journal Title: Mutation Research 560 Pages: 49-55
• [Book] Reviews and Protocols in DT40 Research : Participation of histones, histone modifying enzymes and histone chaperones in vertebrate cell functions (2006)
  • Author(s): Kikuchi, H., Barman, H.K., Nakayama, M., Takami, Y., Nakayama, T.
  • Publisher: Springer-Verlag
  • Description: 「研究成果報告書概要(和文)」より