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Development of multi-channel type surface plasmon resonance sensor and its application to simultaneous determination of allergy related substances

Research Project

Project/Area Number 16350047
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionKYUSHU UNIVERCITY

Principal Investigator

IMATO Toshihiko  Kyushu University, Faculty of Engineering, Professor, 大学院工学研究院, 教授 (50117066)

Co-Investigator(Kenkyū-buntansha) SOH Nobuaki  Kyushu University, Faculty of Engineering, Assistant Professor, 大学院工学研究院, 助手 (90336008)
辺見 彰秀  メビウスアドバンストテクノロジー(有), 代表取締役社長(研究職)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥8,200,000 (Direct Cost: ¥8,200,000)
Keywordssurface plasmon resonance / allergy / immunoassay / microchannel / competitive assay / サンドイッチ測定法
Research Abstract

The propose of this project is to develop a portable surface plasmon resonance sensor with a multi-channel flow cell for allergy diagnosis by using immunoreactions. Especially, histamine and immunoglobulin E (IgE) have proven to play a very important role in the development of allergies in humans. In this project, we have developed a simple and rapid method for the determination of histamine and IgE based on a surface plasmon resonance immunosensor. In addition, we have developed a multi-channel type surface plasmon resonance sensor with a small size. The research results obtained in this project is as follows.
(1)The determination method for histamine has been achieved. A sensor chip for histamine was prepared by immobilization of alkane thiol compounds followed by amino-coupling with histamine. A sample histamine solution containing an anti-histamine antibody at a constant concentration was introduced into the sensor chip. The anti-histamine reacts competitively with the histamine con … More jugate immobilized on the sensor chip and histamine in the sample solution. The resulting anti-histamine antibody bound to the histamine conjugate generated the sensor signal of the SPR sensor. A lower detection limit of 3 ppb histamine was achieved by the proposed method. The sensor chip was found to be possible to use repeatedly by regeneration with an HC1 solution.
(2)The determination method for IgE has been achieved. A sensor chip for IgE was prepared by immobilization of an anti-IgE(D) antibody by physical adsorption. A sample IgE solution containing an excess amount of an anti-IgE(H) antibody was introduce into a sensor chip. IgE in the sample solution was converted to an immunocomplex of IgE-anti-IgE(H) and the resulting immunocomplex is bound to the anti-IgE(D) immobilized on the sensor chip. This sandwich reaction of IgE generated a large sensor signal of the SPR sensor. A lower detection limit 10 ppb IgE has been achieved.
(3)A portable surface plasmon resonance sensor with a multi-channel flow cell has been developed. The size and weight of the sensor was 170(W) x 110(D) x 150(H) mm and about 1.5 kg, respectively. The optical system of the sensor consisted of a light emitting diode (770 nm), cylindrical lens, collection spherical lens, polarizer, reflection mirror and a linear CCD sensor. The performance of the sensor was evaluated by using sucrose solution. A good linear relationship between the sensor signal and the concentration of sucrose was found in the concentration range up to 20 mM. The lower detection limit of 0.3 mM was achieved. This value corresponds 10^<-6> RIU level in refractive index sensitivity. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (25 results)

All 2007 2006 2005 2004

All Journal Article (18 results) Book (4 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Surface plasmon resonance immunosensor for IgE analysis using two types of inti-IgE antibodies with different active recognition sites.2007

    • Author(s)
      Yan Li, Jujie Ren, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Toshihiko Imato
    • Journal Title

      Analytical Sciences 23

      Pages: 1-9

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface plasmon resonance immunosensor for IgE analysis using two types of anti-IgE antibodies with different active recognition sites.2007

    • Author(s)
      Yan Li, Jujie Ren, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Toshihiko Imato
    • Journal Title

      Analytical Sciences 23

      Pages: 1-9

    • NAID

      10018464003

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface plasmon resonance immunosensor for IgE analysis using two types of anti-IgE antibodies with different active recognition sites2007

    • Author(s)
      Yan Li, Jujie Ren, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Toshihiko Imato
    • Journal Title

      Analytical Sciences 23

      Pages: 1-9

    • NAID

      10018464003

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A palm-sized surface plasmon resonance sensor with microchip flow cell2006

    • Author(s)
      Hizuru Nakajima, Yuuki Harada, Yasukazu Asano, Tatsuro Nakagama, Katsumi Uchiyama, Toshihiko Imato, Nobuakai Soh, Akihide Hemmi
    • Journal Title

      Talanta 70

      Pages: 419-425

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction2006

    • Author(s)
      Yan Li, Masatoshi Kobayashi, Koji Furui, Nobuaki Soh, Koji Nakano, Toshihiko Imato
    • Journal Title

      Analytica Chimica Acta 576

      Pages: 77-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface plasmon resonance immunosensor for histamine based on an indirct competitive immunoreaction2006

    • Author(s)
      Y.Li, M.Kobayashi, K.Furui, N.Soh, K.Nakano, T.Imato
    • Journal Title

      Analytica Chimica Acta 576

      Pages: 77-83

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A palm-sized surface plasmon resonance sensor with microchip flow cell2006

    • Author(s)
      Hizuru Nakajima, Yuuki Harada, Yasukazu Asano, Tatsuro Nakagawa, Katsumi Uchiyama, Toshihiko Imato, Nobuakai Soh, Akihide Hemmi
    • Journal Title

      Talanta 70

      Pages: 419-425

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Surface plasmon resonance immunosensor for histamine based on an indirct competitive immunoreaction2006

    • Author(s)
      Y.Li, M.Kobayashi, K.Furui, N.Soh, K.Nakano, T.Imato
    • Journal Title

      Analytica Chimica Acta (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 化学センサの開発とその流れ分析への応用2005

    • Author(s)
      今任稔彦
    • Journal Title

      分析化学 54・12

      Pages: 1123-1136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Flow immunoassay of trinitrophenol based on a surface plasmon resonance sensor using a one-pot immunoreaction with a high molecular weight conjugate2005

    • Author(s)
      Masatoshi Kobayashi, Masahiro Sato, Yan Li, Nobuaki, Soh, Koji Nakano, Kiyoshi Toko, Norio Miura, Kiyoshi Matsumoto, Akihide Hemmi, Yasukazu Asano, Toshihiko Imato
    • Journal Title

      Talanta 68

      Pages: 198-206

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of Chemical Sensors and Its Application to Flow Analysis2005

    • Author(s)
      Toshihiko Imato
    • Journal Title

      Bunseki Kagaku 54

      Pages: 1123-1136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of Palm-Sized Differential Plasmon Resonance Meter Based on Concept of Sprode2005

    • Author(s)
      A.HEMMI, T.IMATO, Y.AOKI, M.SATO, N.SOH, Y.ASANO, C.AKASAKA, S.OKUTANI, S.OHKUBO, N.KANEKI, K.SHIMADA, T.EGUCHI, T.OINUMA
    • Journal Title

      Sensors and Actuators B 108

      Pages: 893-898

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Flow Immunoassay of Trinitrophenol Based on Surface Plasmon Resonance Sensor Using a One-Pot Immunoreaction with a High Molecular Weight Conjugate2005

    • Author(s)
      M.KOBAYASHI, M.SATO, Y.LI, N.SOH, K.NAKANO, K.TOKO, N.MIURA, K.MATSUMOTO, A.HEMMI, Y.ASANO, T.IMATO
    • Journal Title

      Talanta 68

      Pages: 198-206

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Preparation and Characterization of a Polyclonal Antibody from Rabbit for Detection of Trinitrotoluene by a Surface Plasmon Resonance Biosensor2005

    • Author(s)
      K.MATSUMOTO, A.TORIMARU, S.ISHITOBI, T.SAKAI, H.ISHIKAWA, K.TOKO, N.MIURA, T.IMATO
    • Journal Title

      Talanta 68

      Pages: 305-311

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 化学センサーの開発と流れ系分析への応用2005

    • Author(s)
      今任稔彦
    • Journal Title

      分析化学 54・12

      Pages: 1123-1136

    • NAID

      110002973721

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Highly sensitive surface plasmon resonance immunosensor for parts-per-trillion level detection of 2,4,6-trinitrophenol2004

    • Author(s)
      Dhesingh Ravi Shankarana, K.Vengatajalabathy Gobia, Kiyoshi Matsumoto, Toshihiko Imato, Kiyoshi Toko, Norio Miura
    • Journal Title

      Sensors and Actuators B 100

      Pages: 450-454

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A ratiometric Fluorescence probe for imaging hydroxyl radicals in living cells2004

    • Author(s)
      Nobuaki Soh, Koji Makihara, Emino Sakoda, Toshihiko Imato
    • Journal Title

      Chemical Communication

      Pages: 496-497

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Chemiluminescence sequential injection immunoassay for vitellogenin using magnetic microbeads2004

    • Author(s)
      Nobuakai Soh, Hideshi Nishiyama, Yasukazu Asano, Toshihiko Imato, Takashi Masadome, Youichi Kurosawa
    • Journal Title

      Talanta 64・5

      Pages: 1160-1168

    • Related Report
      2004 Annual Research Report
  • [Book] モレキュラーインフォーマティクスを拓く分子機能材料 6.1 分子認識に立脚した光センシング2006

    • Author(s)
      分担執筆 宗 伸明, 今任稔彦, 編著 新海征治
    • Total Pages
      10
    • Publisher
      日刊工業新聞社
    • Related Report
      2006 Annual Research Report
  • [Book] クリスチャン分析化学2005

    • Author(s)
      今任稔彦
    • Total Pages
      44
    • Publisher
      化学量論計算(第5章),環境試料採取と分析(第26章)(翻訳)丸善
    • Related Report
      2005 Annual Research Report
  • [Book] 環境分析化学(分担 : 2章、分析化学に見られる化学平衡)2004

    • Author(s)
      合原真, 今任稔彦, 岩永達人, 氏本菊次郎, 吉塚和治, 脇田久伸 共著
    • Total Pages
      44
    • Publisher
      三共出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 環境分析化学(分担:2章、分析化学に見られる化学平衡)2004

    • Author(s)
      合原真, 今任稔彦, 岩永達人, 氏本菊次郎, 吉塚和治, 脇田久伸 共著
    • Total Pages
      44
    • Publisher
      三共出版
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 表面プラズモン共鳴現象測定装置2004

    • Inventor(s)
      金木則明, 今任稔彦その他4名
    • Industrial Property Rights Holder
      室蘭工業大学, 九州大学, メビウスアドバンストテクノロジー(有)
    • Industrial Property Number
      2004-179841
    • Filing Date
      2004-06-17
    • Acquisition Date
      2006-12-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 表面プラズモン共鳴現象測定装置2004

    • Inventor(s)
      金木則明, 今任稔彦, その他4名
    • Industrial Property Rights Holder
      室蘭工業大学, 九州大学, メビウスアドバンストテクノロジー(有)
    • Industrial Property Number
      2004-179841
    • Filing Date
      2004-06-17
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 表面プラズモン共鳴現象測定装置2004

    • Inventor(s)
      金木 則明, 今任 稔彦, その他4名
    • Industrial Property Rights Holder
      室蘭工業大学, 九州大学, メビウスアドバンストテクノロジー(有)
    • Industrial Property Number
      2004-179841
    • Filing Date
      2004-06-17
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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