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Structural Fluctuation and Function of Proteins Revealed by Compressibility

Research Project

Project/Area Number 16350088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionHIR0SHIMA UNIVERSITY

Principal Investigator

GEKKO Kunihiko  Hiroshima University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10023467)

Co-Investigator(Kenkyū-buntansha) AIDA Misako  Hiroshima University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (90175159)
KATAYANAGI Katsuo  Hiroshima University, Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (20291479)
OHMAE Eiji  Hiroshima University, Graduate School of Science, Research Associate, 大学院・理学研究科, 助手 (30284152)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2005: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2004: ¥8,100,000 (Direct Cost: ¥8,100,000)
Keywordssynchrotron / biomolecules / circular dichroism / vacuum-ultraviolet / structure analysis
Research Abstract

The vacuum-ultraviolet circular dichroism (VUVCD) spectra of various amino acids and proteins were measured using a synchrotron-radiation CD spectrophotometer at HiSOR/HSRC that is capable of measuring the CD spectra down to 140 nm in aqueous solution. L-isomers of amino acids show two successive positive peaks at around 200 and 180 nm depending on the side chain. The ab initio assignment by time-dependent density functional theory predicts that these peaks are due to n-π^* and π-π^* transitions of the carboxyl group, respectively. This analytical method was extended to assign the VUVCD spectra of other amino acids and oligopeptides. The VUVCD spectra of 31 globular proteins allowed us to estimate more accurately the content and number of α-helix and β-strand segments by extending the short-wavelength limit of the analytical program SELCON3 down to 160 nm. VUVCD spectroscopy was also applied for analysis of the structure of denatured proteins that remains not well characterized. The VUVCD spectra of metmyoglobin, staphylococcal nuclease, and thioredoxin down to 172 nm showed characteristic changes depending on the solvent conditions such as pH, temperature, and additives (to be published). Analysis using the SELCON3 program revealed quantitative differences in the secondary structures of the acid-, cold-, heat-, and guanidine-hydrochloride-denatured proteins. These results demonstrate that synchrotron-radiation VUVCD spectroscopy is a useful tool for structure analyses of biomolecules in solution based on the higher energy transitions of chromophores.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2006 2005 2004 Other

All Journal Article (21 results)

  • [Journal Article] Vacum-Ultraviolet Crcular Dichroism Analysis of Biomolecles2006

    • Author(s)
      Kunihiko Gekko
    • Journal Title

      Chirality 18

      Pages: 329-334

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vacuum-Ultraviolet Circular Dichroism Analysis of Biomolecules.2006

    • Author(s)
      Kunihiko Gekko
    • Journal Title

      Chirality 18

      Pages: 329-334

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Improved Estimation of the Secondary Structures of Proteins by Vacuum-Ultraviolet Circular Dichroism Spectroscopy2005

    • Author(s)
      Koichi Matsuo
    • Journal Title

      J. Biochem. 138

      Pages: 79-88

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vacuum-Ultraviolet Electronic Circular Dichroism of L-Alanine in Aqueous Solition Investigated by Time-Dependent Density Functional theory2005

    • Author(s)
      Takayuki Fuluyama
    • Journal Title

      J. Phys. Chem.,A 109

      Pages: 6928-6933

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vacum-Ultraviolet Circular Dichroism Sprectrophotometerusing Symchrotron Radiation2005

    • Author(s)
      Koichi Matsuo
    • Journal Title

      J. Electron Spectros. Relat. Phenomena 144-147

      Pages: 1023-1025

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structure Analyses of Biomolecules using Synchrotron Radiation Circular Dichroism Spectrophotometer2005

    • Author(s)
      Kunihiko Gekko
    • Journal Title

      J. Electron Spectros. Relat. Phenomena 144-147

      Pages: 295-297

    • NAID

      10012362882

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Mutation at Methionine-42 of Escherichiia colo Dihydrofolate Reductase on Stability and Function : Implication of Hydrophobic Interactions2005

    • Author(s)
      Eiji Ohmae
    • Journal Title

      J. Biochem. 137

      Pages: 643-652

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Improved Estimation of the Secondary Structures of Proteins by Vacuum-Ultraviolet Circular Dichroism Spectroscopy.2005

    • Author(s)
      Koichi Matsu
    • Journal Title

      J.Biochem. 138

      Pages: 79-88

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vacuum-Ultraviolet Electronic Circular Dichroism of L-Alanine in Aqueous Solution Investigated by Time-Dependent Density Functional Theory.2005

    • Author(s)
      Takayuki Fukuyama
    • Journal Title

      J.Phys.Chem., A 109

      Pages: 6928-6933

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vacuum-Ultraviolet Circular Dichroism Spectrophotometer using Synchrotron Radiation.2005

    • Author(s)
      Koichi Matsuo
    • Journal Title

      J.Electron Spectros. Relat.Phenomena 144-147

      Pages: 1023-1025

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structure Analyses of Biomolecules using Synchrotron Radiation Circular Dichroism Spectrophotometer.2005

    • Author(s)
      Kunihiko Gekko
    • Journal Title

      J.Electron Spectros.Relat.Phenomena 144-147

      Pages: 295-297

    • NAID

      10012362882

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Mutation at Methionine-42 of Escherichia coil Dihydrofolate Reductase on Stability and Function : Implication of Hydrophobic Interactions.2005

    • Author(s)
      Eiji Ohmae
    • Journal Title

      J.Biochem. 137

      Pages: 643-652

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Improved Estimation of the Secondary Structures of Proteins by Vacuum-Ultraviolet Circular Dichroism Spectroscopy2005

    • Author(s)
      Koichi Matsuo
    • Journal Title

      J.Biochem. 138

      Pages: 79-88

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Vacuum-Ultraviolet Electronic Circular Dichroism of L-Alanine in Aqueous Solution Investigated by Time-Dependent Density Functional Theory2005

    • Author(s)
      Takayuki Fukuyama
    • Journal Title

      J.Phys.Chem., A 109

      Pages: 6928-6933

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Vacuum-Ultraviolet Circular Dichroism Spectrophotometer using Synchrotron Radiation2005

    • Author(s)
      Koichi Matsuo
    • Journal Title

      J.Electron Spectros.Relat.Phenomena 144-147

      Pages: 1023-1025

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structure Analyses of Biomolecules using Synchrotron Radiation Circular Dichroism Spectrophotometer2005

    • Author(s)
      Kunihiko Gekko
    • Journal Title

      J.Electron Spectros.Relat.Phenomena 144-147

      Pages: 295-297

    • NAID

      10012362882

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of Mutation at Methionine-42 of Escherichia coli Dihydrofolate Reductase on Stability and Function : Implication of Hydrophobic Interactions2005

    • Author(s)
      Eiji Ohmae
    • Journal Title

      J.Biochem. 137

      Pages: 643-652

    • NAID

      10017345265

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Vacuum-Ultraviolet Circular Dichroism Spectrophotometer using Synchrotron Radiation2005

    • Author(s)
      Koichi Matsuo
    • Journal Title

      J.Electron Spectros.Relat.Phenomena (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structure Analyses o Biomolecules using Synchrotron Radiation Circular Dichroism Spectrophotometer2005

    • Author(s)
      Kunihiko Gekko
    • Journal Title

      J.Electron Spectros.Relat.Phenomena (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 放射光真空紫外円二色性分散計による生体物質の構造解析2004

    • Author(s)
      月向邦彦
    • Journal Title

      日本放射光学会誌 17

      Pages: 117-225

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Selective Metal Binding to Vanabin2 in the Vanadium-rich Ascidian, Ascidia sydneiensis samea

    • Author(s)
      Norifumi Kawakami
    • Journal Title

      Biochim.Bophys.Acta (印刷中)

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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