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Spatio-temporally resolved observation and control of neuron excitement mechanism using nonlinear laser microscopes

Research Project

Project/Area Number 16360024
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied optics/Quantum optical engineering
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MORITA Ryuji  Hokkaido Univ., Grad.School of Eng., Prof., 大学院工学研究科, 教授 (30222350)

Co-Investigator(Kenkyū-buntansha) GOHARA Kazutoshi  Hokkaido Univ., Grad.School of Eng., Prof., 大学院工学研究科, 教授 (40153746)
TODA Yashinori  Hokkaido Univ., Grad.School of Eng., Assoc.Prof, 大学院工学研究科, 助教授 (00313106)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordsnonlinear laser microscope / inversion-symmetry breaking / living cell / neuron / optical second-harmonic wave / coherent anti-Stokes Raman scattering / DNA / 3-dimensional spatially-resolving / 光第2高調波顕微鏡 / 超広帯域スペクトル / フォトニック結晶ファイバー / スペクトル干渉 / レーザー走査トンネル顕微鏡
Research Abstract

1.An over-one-octave spanning (460-1060 nm) ultra-broadband optical pulse was generated by self-modulation effect. It was spectrally-phase-compensated and characterized to be a 2.8 fs, 1.5 cycle-Fourier-transform-limited pulse by the modified spectral phase interferometry for direct electric-field reconstruction. It is the shortest visible and infrared pulse whose spectral phase is well determined.
2.A second-harmonic laser microscope was developed for 3-dimensional spatially-resolved observation of living cells. As an example, neurons of a rat were observed. Strong signals from nuclei and axons owing to inversion-symmetry breaking were detected. Spatial resolution was less than 1.5 μm.
3.A coherent anti-Stokes Raman scattering laser microscope was developed using a femtosecond laser amplifier as a light source. For onion cells, two pulses with wavelengths of 800 and 1040 nm were employed to excite the C-H vibration mode of 2900 cm-1 and the corresponding spatially-resolved signal with a wavelength of 650 nm was detected. Similarly, two pulses with wavelengths of 800 and 1100 nm were employed to excite the N-H vibration mode of 3400 cm-1 and the corresponding signal with a wavelength of 630 nm was detected. From comparison between those signals, C-H-and N-H-rich domains in cells were determined.
4.Macroscopic observation of hybridization and dehybridization for methylred-intercalated DNA was performed using second-harmonic laser microscope. While hybridized one has the inversion symmetry, dehybridized one does not. Significant signals were not obtained so far because of noise. Improvement is going on by introducing other azo-dyes with higher optical nonlinearity.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (38 results)

All 2007 2006 2005 2004

All Journal Article (36 results) Book (2 results)

  • [Journal Article] Coherent double-pulse excitation of charge-density-wave oscillation2007

    • Author(s)
      T.Onozaki et al.
    • Journal Title

      Jpn. J. Appl. Phys. 46・2

      Pages: 870-872

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coherent double-pulse excitation of charge-density-wave oscillation2007

    • Author(s)
      T.Onozaki et al.
    • Journal Title

      Jpn.J.Appl.Phys. 46-2

      Pages: 870-872

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coherent double-pulse excitation of charge-density-wave oscillation2007

    • Author(s)
      T.Onozaki, et al.
    • Journal Title

      Jpn. J. Appl. Phys. 46・2

      Pages: 870-872

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Quasi-automatic phase-control technique for chirp compensation of pulses with over-one-octave bandwidth-generation of few- to mono-cycle optical pulses2006

    • Author(s)
      M.Yamashita et al.
    • Journal Title

      IEEE J. Sel. Top. Quantum Electron. 12・2

      Pages: 213-222

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Direct observation of Gouy phase shift in a propagating optical vortex2006

    • Author(s)
      J.Hamazaki et al.
    • Journal Title

      Opt. Express 14・18

      Pages: 8382-8392

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quasi-automatic phase-control technique for chirp compensation of pulses with over-one-octave bandwidth---generation of few-to mono-cycle optical pulses2006

    • Author(s)
      M.Yamashita et al.
    • Journal Title

      IEEE J.Sel.Top.Quantum Elctron. 12-2

      Pages: 213-222

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Direct observation of Gouy phase shift in a propagating optical vortex2006

    • Author(s)
      J.Hamazaki et al.
    • Journal Title

      Opt.Express 14-18

      Pages: 8382-8392

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Direct observation of Gouy phase shift in a propagating optical vortex2006

    • Author(s)
      J.Hamazaki, et al.
    • Journal Title

      Opt. Express 14・18

      Pages: 8382-8392

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Quasi-automatic phase-control technique for chirp compensation of pulses with over-one-octave bandwidth---generation of few-to mono-cycle optical pulses2006

    • Author(s)
      M.Yamashita, et al.
    • Journal Title

      IEEE J. Sel. Top. Quantum Electron. 12・2

      Pages: 213-222

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Quasi-automatic phase-control technique for chirp compensation of pulses with over-one-octave bandwidth--generation of few- to mono-cycle optical pulses2006

    • Author(s)
      M.Yamashita et al.
    • Journal Title

      IEEE J.Sel.Top.Quantum Electron. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Wavelet-transform analysis of spectral shearing interferometry for phase reconstruction of femtosecond optical pulses2005

    • Author(s)
      Y.Deng et al.
    • Journal Title

      Opt. Express 13・6

      Pages: 2120-2126

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Extended finite difference time domain analysis of induced phase modulation and four-wave mixing between two-color femtosecond laser pulses in a silica fiber with different initial delays2005

    • Author(s)
      S.Nakamura et al.
    • Journal Title

      Jpn. J. Appl. Phys. 44・10

      Pages: 7453-7459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sub-5-fs pulse compression of laser output using photonic crystal fiber with short zero-dispersion wavelength2005

    • Author(s)
      M.Adachi et al.
    • Journal Title

      Jpn. J. Appl. Phys. 44・47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mono-cycle photonics and optical scanning tunneling microscopy ---route to femtosecond-angstrom technology---2005

    • Author(s)
      M.Yamashita et al.
    • Journal Title

      Springer Verlag 389

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Wavelet-transform analysis of spectral shearing interferometry for phase reconstruction of femtosecond optical pulses2005

    • Author(s)
      Y.Deng et al.
    • Journal Title

      Opt.Express 13-6

      Pages: 2120-2126

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Extended finite difference time domain analysis of induced phase modulation and four-wave mixing between two-color femtosecond laser pulses in a silica fiber with different initial delays2005

    • Author(s)
      S.Nakamura et al.
    • Journal Title

      Jpn.J.Appl.Phys. 44-10

      Pages: 7453-7459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sub-5-fs pulse compression of laser output using photonic crystal fiber with short zero-dispersion wavelength2005

    • Author(s)
      M.Adachi et al.
    • Journal Title

      Jpn.J.Appl.Phys. 44-47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sub-5-fs pulse compression of laser output using photonic crystal fiber with short zero-dispersion wavelength2005

    • Author(s)
      M.Adachi et al.
    • Journal Title

      Jpn.J.Appl.Phys. 44・47

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Extended finite difference time domain analysis of induced phase modulation and four-wave mixing between two-color femtosecond laser pulses in a silica fiber with different initial delays2005

    • Author(s)
      S.Nakamura et al.
    • Journal Title

      Jpn.J.Appl.Phys. 44・10

      Pages: 7453-7459

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Wavelet-transform analysis of spectral shearing interferometry for phase reconstruction of femtosecond optical pulses2005

    • Author(s)
      Y.Deng et al.
    • Journal Title

      Opt.Express 13・6

      Pages: 2120-2126

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Selective excitation of self-assembled quantum dots by using shaped pulses2004

    • Author(s)
      Toda et al.
    • Journal Title

      Physica E 21・2-4

      Pages: 180-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pulse compression of white-light continuum generated by indeced phase modulation in a conventional glass fiber2004

    • Author(s)
      M.Yamashita et al.
    • Journal Title

      J. Opt. Soc. Am. B 21・2

      Pages: 458-462

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pulse compression using direct feedback of the spectral phase from photonic crystal fiber output without the need for the Taylor expansion method2004

    • Author(s)
      M.Adachi et al.
    • Journal Title

      IEEE Photonics Technol. Lett. 16・8

      Pages: 1951-1953

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coherent optical control of molecular motion using polarized sequential pulses2004

    • Author(s)
      K.Itoh et al.
    • Journal Title

      Jpn. J. Appl. Phys. 43・9A

      Pages: 6448-6451

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Experimental and theoretical demonstration of validity and limitations in fringe-resolved autocorrelation measurements for pulses of few optical cycles2004

    • Author(s)
      K.Yamane et al.
    • Journal Title

      Opt. Express 12・12

      Pages: 2762-2773

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Probing subpicosecond dynamics using pulsed laser combined scanning tunneling microscopy2004

    • Author(s)
      O.Takeuchi et al.
    • Journal Title

      Appl. Phys. Lett. 85・15

      Pages: 3268-3270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Selective excitation of self-assembled quantum dots by using shaped pulses2004

    • Author(s)
      Toda et al.
    • Journal Title

      Physica E 21-2-4

      Pages: 180-183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pulse compression of white-light continuum generated by indeced phase modulation in a conventional glass fiber2004

    • Author(s)
      M.Yamashita et al.
    • Journal Title

      J.Opt.Soc.Am.B 21-2

      Pages: 458-462

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pulse compression using direct feedback of the spectral phase from photonic crystal fiber output without the need for the Taylor expansion method2004

    • Author(s)
      M.Adachi et al.
    • Journal Title

      IEEE Photonics Technol.Lett. 16-8

      Pages: 1951-1953

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coherent optical control of molecular motion using polarized sequential pulses2004

    • Author(s)
      K.Itoh et al.
    • Journal Title

      Jpn.J.Appl.Phys. 43-9A

      Pages: 6448-6451

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Experimental and theoretical demonstration of validity and limitations in fringe-resolved autocorrelation measurements for pulses of few optical cycles2004

    • Author(s)
      K.Yamane et al.
    • Journal Title

      Opt.Express 12-12

      Pages: 2762-2772

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Probing subpicosecond dynamics using pulsed laser combined scanning tunneling microscopy2004

    • Author(s)
      O.Takeuchi et al.
    • Journal Title

      Appl.Phys.Lett. 85-15

      Pages: 3268-3270

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Probing subpicosecond dynamics using pulsed laser combined scanning tunneling microscopy2004

    • Author(s)
      O.Takeuchi et al.
    • Journal Title

      Appl.Phys.Lett. 85・15

      Pages: 3268-3270

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Experimental and theoretical demonstration of validity and limitations in fringe-resolved autocorrelation measurements for pulses of few optical cycles2004

    • Author(s)
      K.Yamane et al.
    • Journal Title

      Opt.Express 12・12

      Pages: 2762-2773

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Coherent optical control of molecular motion using polarized sequential pulses2004

    • Author(s)
      K.Itoh et al.
    • Journal Title

      Jpn.J.Appl.Phys. 43・9A

      Pages: 6448-6451

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pulse compression using direct feedback of the spectral phase from photonic crystal fiber output without the need for the Taylor expansion method2004

    • Author(s)
      M.Adachi et al.
    • Journal Title

      IEEE Photonics Technol.Lett. 16・8

      Pages: 1951-1953

    • Related Report
      2004 Annual Research Report
  • [Book] Mono-cycle photonics and optical scanning tunneling microscopy-route to femtosecond-angstrom technology-2005

    • Author(s)
      M.Yamashita et al.
    • Total Pages
      389
    • Publisher
      Springer Verlag
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Mono-Cycle Photonics and Optical Scanning Tunneling Microscopy --Route to Femtosecond Ångstrom Technology--2005

    • Author(s)
      M.Yamashita, H.Shigekawa, R.Morita
    • Total Pages
      389
    • Publisher
      Springer Verlag
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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