| Project/Area Number |
16360407
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
KOBATAKE Eiry Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, Associate Professor, 大学院生命理工学研究科, 助教授 (00225484)
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| Co-Investigator(Kenkyū-buntansha) |
MIE Masayasu Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, Assistant Professor, 大学院生命理工学研究科, 助手 (40334528)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | biosensing / cell / histamine / biotechnology / protein / 生体材料 / 生物生体工学 / 蛋白質 / 細胞バイオセンシング / インテリジェント材料 / 疎水性人工タンパク質 / 一酸化窒素 / 細胞接着タンパク質 |
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| Research Abstract |
Cells can growth and differentiate by the effect of some growth factors. Cells also have various intelligent systems such as self-diagnosis and self-repair. Therefore, we have a possibility to realize advanced intelligent biosensing systems through the use living cells as materials. In this study, we purposed to construct an intelligent cellular biosensing system which can evaluate the effects of chemicals to a living body.
We had already established a cellular biosensing system for assessing chemicals which have the effect to the endothelial cells by using NO released from cells. In this year, we tried to construct a novel cellular biosensing system for the detection of allergens. For this purpose, we utilized a histamine oxidase. At first, histamine oxidase was expressed in E.coli and purified by affinity chromatography. Some electrochemical characterizations were then performed. Finally, we constructed a biosensing system by the combination of cells and histamine oxidase-immobilized electrode. We could detect histamine which was released from cells by the stimulation of chemicals.
From these results, it is clarified that constructing intelligent cellular biosensing system is useful to evaluate the effects of chemicals to a living body.
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