| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2005: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2004: ¥9,700,000 (Direct Cost: ¥9,700,000)
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| Research Abstract |
Biodegradation experiments of oil have been conducted at 20MPa and 279 and 298K to simulate ship wrecks in deep sea. About 100m1 of natural sea water, taken at Tokyo bay or Sagami bay, was mixed with 0.4ml of oil for 10 to 75days. Phosphate and Ammonium salts were added to the sea water. In most of the runts either n-decane or n-hexadecane was used as the oil. After a certain time period of the mixing, the degradation rate was determined by comparing the concentration rations of originally added oil to the other n-alkane added as an internal standard substance after the runs. The concentration was determined with GC-MS. The experimental results show fluctuation of the degradation process. In one case, more than 50% of the oil was decomposed in 10 days, but in another case almost no degradation was observed. The change in DNA sequence of the microcosm in the water was analyzed by DGGE-PCR. The band pattern after the degradation experiment was changed from that in the original sea water, and in each experiment the band patterns were different. It has not been successful to identify the microcosm which contributes the degradation of oil in high pressure water.
A data base for the ship wrecks near Japanese islands has been constructed. The wrecks over 100 tonnage in this about 100 years have been investigated and 1,206 of them are filed in the data base. The data included are name, type, tonnage and cargo of the ships, date, position and cause of the accidents, damage on the ship structure, human damage, depth of the wreck, size, date of built, owner or operator, shipbuilder of the ships and treatment after the accident.
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