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Molecular dissection of the 26S proteasome in the budding yeast

Research Project

Project/Area Number 16370001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionThe University of Tokyo

Principal Investigator

TOH-E Akio  The University of Tokyo, Graduate School of Sciences, Professor, 大学院・理学系研究科, 教授 (90029249)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥6,400,000 (Direct Cost: ¥6,400,000)
Keywords26S proteasome / lid / base / temperature-sensitive mutants / hierarchy of assembly / 19SRP / yeast / ユビキチン・プロテアソーム系 / サブユニット集合 / RPN5 / RPN89 / 局在 / 26Sプアロテアソーム / 19S RP / RPN6 / RPN7 / 蛋白質分解
Research Abstract

It is widely accepted that the ubiquitin-proteasome system playas a pivotal role in degradation of short-lived proteins, many of them are regulatory proteins, in an energy dependent manner. The 26S proteasome is responsible to the final step of proteolysis in this system. The 26S proteasome is a large protein complex consisting of 20S proteasome containing active sites and 19S regulatory factor called as 19S RP. The basic subunit composition has been elucidated. By exploiting this information, we started uncovering how and where these subunits assemble together. We focused on the 19S RP of Saccharomyces cerevisiae. The 19S RP consisted of two sub-complexes, the lid and the base. We isolated temperature-sensitive mutants of the genes encoding subunits of the lid.Biochemical characterization revealed that hierarchy of the assembly of the lid and the lid and the base are constructed independently of each other. We also examined localization of each sub-complex in various mutants and found that the base and the lid can separately be localized in the nucleus. The 19S RP too is localized in the nucleus and assembles with the 20S proteasome to the 26S proteasome.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (34 results)

All 2005 2004

All Journal Article (34 results)

  • [Journal Article] Las24/KOGI,a component of the TOR complex 1(TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast.2005

    • Author(s)
      Araki, T.
    • Journal Title

      Genes Genet. Syst. 80

      Pages: 325-343

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae.2005

    • Author(s)
      Isono, E.
    • Journal Title

      J. Biol. Chem. 280

      Pages: 6537-6547

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically encodable tandem ubiquitin.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method. Enzymol. 399

      Pages: 64-74

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method. Enzymol. 399

      Pages: 215-227

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Las24/KOG1, a component of the TOR complex 1(TORC1), is needed for resistance to local anesthetic tetracaine and normal of distribution of actin cytoskeleton in yeast.2005

    • Author(s)
      Araki, T.
    • Journal Title

      Genes Genet.Syst. 80

      Pages: 325-343

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional analysis of Rpn6p, a lid componenet of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae.2005

    • Author(s)
      Isono, E.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 6537-6547

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically encodable tandem ubiquitin.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method.Enzymol 399

      Pages: 64-74

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method.Enzymol 399

      Pages: 215-227

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae2005

    • Author(s)
      Isono, E., Saito, N., Kamata, N., Saeki, Y., Toh-e, A.
    • Journal Title

      J.Biol.Chem. 280・8

      Pages: 6537-6547

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically encodable tandem ubiquitin.2005

    • Author(s)
      Saeki, Y., Isono, E., Shimada, M., Kawahara, H., Yokosawa, H., Toh-e, A.
    • Journal Title

      Method.Enzymol. 399

      Pages: 64-74

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity. Method.2005

    • Author(s)
      Saeki, Y., Isono, E., Toh-e, A.
    • Journal Title

      Method.Enzymol. 399

      Pages: 215-227

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of yeast Saccharomyces cerevisiae2005

    • Author(s)
      Isono, E., Saito, N., Kamata, N., Saeki, Y., Yoh-e, A.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 6537-6547

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rpn7 is required for the structural integrity of the 26S proteasome of Saccharomyces cervisiae.2004

    • Author(s)
      Isono, E.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 27168-27176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Biochem. Biophys. Res. Commun. 320

      Pages: 840-845

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Genes Genet. Syst. 79

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Semlp is a novel subunit of the 26S proteasome from Saccharomyces cerevisiae.2004

    • Author(s)
      Sone, T.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 28807-28816

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Cell Struc. Funct. 29

      Pages: 1-15

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simultaneous yet independent regulation of actin cytoskeletal organization and translation initiation by glucose in Saccharomyces cerevisiae.2004

    • Author(s)
      Uesono, Y.
    • Journal Title

      Mol. Biol. Cell 15

      Pages: 1544-1556

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mmrlp is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast2004

    • Author(s)
      Itoh, T.
    • Journal Title

      EMBO J. 23

      Pages: 2520-2530

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Forchlorfenuron, a phenylurea cytokinin, disturbs septin organization in Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Genes Genet. Syst. 79

      Pages: 199-206

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Yeast Pho85 kinase is required for proper gene expression during the diauxic shift.2004

    • Author(s)
      Nishizawa, M.
    • Journal Title

      Yeast 21

      Pages: 903-918

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rpn7 is required for the structural integrity of the 26S proteasome of Saccharomyces cervisiae.2004

    • Author(s)
      Isono, E.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 27168-27176

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 320

      Pages: 840-845

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Genes Genet.Syst. 79

      Pages: 77-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Semlp is a novel subunit of the 26S proteasome from Saccharomyces cerevisiae.2004

    • Author(s)
      Sone, T
    • Journal Title

      J.Biol.Chem. 279

      Pages: 28807-28816

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Cell Struc.Funct. 29

      Pages: 1-15

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simultaneous yet independent regulation of actin Cytoskeletal organization and translation initiation by glucose in Saccharomyces cerevisiae.2004

    • Author(s)
      Uesono, Y.
    • Journal Title

      Mol.Biol.Cell 15

      Pages: 1544-1556

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast.2004

    • Author(s)
      Itoh, T.
    • Journal Title

      EMBO J. 23

      Pages: 2520-2530

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Forchlorfenuron, a phenylurea cytokinin, disturbs septin organization in Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Genes Genet.Syst. 79

      Pages: 199-206

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Yeast Pho85 kinase is required for proper gene expression during the diauxic shift.2004

    • Author(s)
      Nishizawa
    • Journal Title

      Yeast 21

      Pages: 903-918

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rpn7 is required for the structural integrity of the 26S proteasome of Saccharomyces cerevisiae.2004

    • Author(s)
      Isono, E., Saeki, Y., Yokosawa, H., Toh-e, A.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 27168-27176

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys 48 Linkage2004

    • Author(s)
      Saeki, Y., Tayama, Y., Toh-e, a., Yokosawa, H.
    • Journal Title

      Biochem Biophys.Res.Commen. 320

      Pages: 840-845

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Intracellularly inducible, ubibquitin hydrolase-insensitive tandemubiquitin inhibits the 26S proteasome activity and cell division.2004

    • Author(s)
      Saeki, Y., Isono, E., Oguchi, T., Shimada, M., Sone, T., Yokosawa, H.
    • Journal Title

      Gene.Genet.Sys. 79

      Pages: 77-86

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Semlp is a novel subunit of the 26S proteasome from Saccharomyces cerevisiae2004

    • Author(s)
      Sone, T., Saeki, Y., Toh-e, A., Yokokawa, H.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 28807-28816

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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