| Project/Area Number |
16370003
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kyoto University |
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Principal Investigator |
MURAKAMI Yota Kyoto University, Institute for Virus Research, Associate Professor, ウイルス研究所, 助教授 (20260622)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Katsunori Shimane University, Facul.of Life and Environmental Science, Associate Professor, 生物資源科学部, 助教授 (60273926)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2005: ¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 2004: ¥8,900,000 (Direct Cost: ¥8,900,000)
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| Keywords | heterochromatin / RNAi / RNA polymerase II / fission yeast / サイレントクロマチン |
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| Research Abstract |
Heterochromatin is a transcriptionally inert higher-order chromatin structure that contributes to global transcriptional repression and genome integrity. In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery converts pericentromeric non-coding RNAs into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications, in the dislocalization of RITS (RNA-induced transcriptional silencing) complex and RDRC (RNA-directed RNA polymeraxe complex), and in the accumulation of pericentromeric non-coding RNAs, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.
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