Molecular mechanism of homologous recombinational repair in Schizosaccharomyces pombe
| Project/Area Number |
16370006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Yokohama City University |
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Principal Investigator |
IWASAKI Hiroshi Yokohama City University, International Graduate School of Arts and Sciences, Professor, 国際総合科学研究科, 教授 (60232659)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2006: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2005: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2004: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Schizosaccharomyces pombe / homologous recombination / DNA double strand breaks / genome integrity / Rhp51 / Swi5 / Sfr1 / DNA repair / swi5 |
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| Research Abstract |
To understand molecular mechanisms of recombination repair system in Schizosaccharomyces pombe, we analyzed "slr" mutants (synthetic lethal with rad2) and Swi5-Sfrl complex.
Project 1. Molecular analyses of slr mutant.
1-1. slr4^+is rad62^+ :
The slr^4+/rad62^+gene encodes one of a non-SMC subunits of SMC5-SMC6 proteins. It directly interacts with Smc5 subunits. Genetic analyses suggest that Rad62, in conjunction with SMC5/6, plays an essential role in recovery from stalled replication by recombination (ref. 1).
1-2. slr3^+ is mcl1^+ :
slr3^+/mcl1^+ is required for chromosome replication, cohesion and segregation. We suggest that Mcl1 is a functional S.cerevisiae Ctf4 homologue and plays in lagging-strand synthesis, in addtion to DNA repair (ref. 2).
1-3. slr5^+ is fbh1^+ :
slr.5^+/fbh1^+ encodes an F-box containing DNA helicase. We suggest that Fbh1 helicase is involved in processing in toxic intermediates formed during Rhp51-dependet recombination (ref. 3).
Project 2. Molecular analysis of Swi5-Sfr1 complex
Subnuclear localizations of Siw5 and Sfr1 and their mutual dependency suggest that-the Swi5/Sfr1 complex is a loading and/or stabilizing factor for the Rhp51 recombinase (ref. 5). There-strand exchange reaction assay using the purified protein components provides the robust evidence that Swi5-Sfr1 works as a recombination mediator. In addition, we demonstrated that it works as a mediator for Dmc1, a meiosis-specific recombinase (ref. 4). To reveal the functional differences between two mediators, we adopted an assay for repair products by an HO endonuclease-induced DSB. The result reveals that Rhp51 and Rhp57are essential, but Swi5/Sfr1 is not essential, for crossover production, indicating that two different mediator, Rhp55/57 and Swi5/Sfr1, process the DSB in different manner (ref. 5).
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.2007
Author(s)
Akamatsu Y, Tsutsui Y, Morishita T, Siddique, MDP, Kurokawa, Y, Ikeguchi M, Fumiaki Yamao F, Arcangioli B, Iwasaki H
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Journal Title
EMBO J. 26
Pages: 1352-1362
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Fission yeast Swi5/Sfrl and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.2007
Author(s)
Akamatsu Y, Tsutsui Y, Morishita T, Siddique, MDP, Kurokawa, Y., Ikeguchi M, Fumiaki Yamao F, Arcangioli B, Iwasaki H
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Journal Title
EMBO J. 26
Pages: 1352-1362
Related Report
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[Journal Article] Role of the Schizosaccharomyces pombe F-box DNA helicase in processing recombination intermediates. Morishita T, Furukawa F, Sakaguchi C, Toda T, Carr AM, Iwasaki H and Shinagawa H.2005
Author(s)
Morishita T, Furukawa F, Sakaguchi C, Toda T, Carr AM, Iwasaki H, Shinagawa H
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Journal Title
Mol Cell. Biol 25
Pages: 8074-8883
Description
「研究成果報告書概要(和文)」より
Related Report
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