Studies on Flower Color Development in Colored cells
Project/Area Number |
16370021
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
植物生理・分子
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Research Institution | Nagoya University |
Principal Investigator |
YOSHIDA Kumi Nagoya University, Graduate School of Information Science, Associate Professor (90210690)
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Co-Investigator(Kenkyū-buntansha) |
MAESHIMA Masayosbi Nagoya University, Graduate School of Bioagricuotuial Sciences, Professor (80181577)
近藤 忠雄 名古屋大学, 大学院・生命農学研究科, 教授 (70093028)
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Project Period (FY) |
2004 – 2007
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Project Status |
Completed (Fiscal Year 2007)
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Budget Amount *help |
¥16,020,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥720,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥7,200,000 (Direct Cost: ¥7,200,000)
|
Keywords | blue flower color development / vacuolar membrane / ion content in vacuole / vacuolar pH / Imopore tricolor / Hydrangea macrophylla / NHX1 / flower color change / Iopmoea tricolor / アントシアニン / 液胞内イオン濃度 / 液胞pH上昇 / 細胞体積 / ItNHX1 / NXH / 空色西洋アサガオ / V-ATPase / V-PPAse / N^+ / H^+対向輸送 |
Research Abstract |
The various tonoplast membrane transports play an important role for flower color development in living petal cells. Therefore, from this point of view, I aimed at the elucidation of the mechanism of flower color development and change by focusing on the tonoplast membrane transport and vacuolar contents of the colored cells, especially blue flowers such as morning glory and hydrangea. (1) Blue morning glory, Ipomoet tricolor cv. Heavenly Blue I prepared colored protoplasts by the enzyme treatment of the petals and isolated tonoplast membrane vesicles. I analyzed a membrane transport proteins concerned with vacuolar pH control. Activities of V-ATPse and V-PPase up-regulated at the last flowering period. The expression of a sodium-proton exchanger (ItNHX1 )increased dramatically on the last stage, and, the activity also increased. These synchronized work of proton pumps and ItNHX1 may affect on pHv increase with a fully-opened blue flower. Furthermore, the volumes of the outer layer cell of the petal of the morning glory increased during flowering. By analysis of ion contents in colored cells clarified the increase of K'. ItNHX1 may exchange not Na' but K' with it. (2) Other flowers The mechanism of blue color development of hydrangea, facelia and tulip were investigated.
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Report
(5 results)
Research Products
(101 results)
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[Journal Article] Perianth bottom-specific blue color development in tulip cv. Murasakizuisho requires ferric ions.2007
Author(s)
Shoji, K., Miki, N., Nakajima, N., Momonoi, K., Kato, C., Yoshida, K.
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Journal Title
Plant Cell Physiol. 48
Pages: 243-251
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Perianth bottom-specific blue color development in tulip cv. Murasakizuisho requires ferric ions2007
Author(s)
Shoji, K, Mild, N., Nakajima, N., Momonoi, K, Kato, C., Yoshida, K
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Journal Title
Plant Cell Physiol 48
Pages: 243-251
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Perianth bottom-specific blue color development in tulip cv. Murasakizuisho requires ferric ions.2007
Author(s)
Shoji, K., Miki, N., Nakajima, N., Momonoi, K., Kato, C., Yoshida, K.
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Journal Title
Plant Cell Physiollogy 48
Pages: 243-251
NAID
Related Report
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[Journal Article] A novel and efficient synthesis of cyanidin 3-O-β-D-glucoside from(+)-catechin via a flay-3-en-3-ol as a key intermediate.2006
Author(s)
Kondo, T., Oyama, K., Nakamura, S., Yamakawa, D., Tokuno, K., Yoshida, K.
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Journal Title
Organic Lett. 8
Pages: 3609-3612
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] A novel and efficient synthesis of cyanidin 3-0-β-D-glucoside from (+)-catechin via a flav-3-en-3-ol as a key intermediate2006
Author(s)
Kondo, T., Oyama, K., Nakamura, S., Yamakawa, D., Tokuno, K., Yoshida, K
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Journal Title
Organic Lett 8
Pages: 3609-3612
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Anthocyanins from the Rhizome of Raphanus sativus, and change in the composition during maturation2006
Author(s)
Mori., M., Nakagawa, S., Maeshima, M., Niikura, S., Yoshida, K
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Journal Title
Heterocylces 69
Pages: 239-251
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] A novel and efficient synthesis of cyanidin 3-O-β-D-glucoside from(+)-catechin via a flav-3-en-3-ol as a key intermediate.2006
Author(s)
Kondo, T., Oyama, K., Nakamura, S., Yamakawa, D., Tokuno, K., Yoshida, K.
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Journal Title
Organic Letters 8
Pages: 3609-3612
Related Report
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[Journal Article] UV-B protecting effect of a polyacylated anthocyanin, HBA, in flower petals of the blue morning glory, Ipomoea tricolor, cv. Heavenly blue.2005
Author(s)
Mori, M., Yoshida, K., Ishigaki, Y., Matsunaga, T., Nikaido, O., Kameda, K., Kondo, T.
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Journal Title
Bioorg. Med. Chem. 13
Pages: 2015-2020
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] The involvement of tonoplast proton pumps and Na^+(K^+)/H^+ exchangers in the change of petal color during flower opening of morning glory, Ipomoea tricolor cv. Heavenly Blue.2005
Author(s)
Yoshida, K., Kawachi, M., Mori, M., Maeshima, M., Kondo, M., Nishimura M., Kondo, T.
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Journal Title
Plant Cell Physiol. 46
Pages: 407-415
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] UV-B protecting effect of a polyacylated anthocyanin, HBA, in flower petals of the blue morning glory, Ipomoea tricolor, cv. Heavenly blue2005
Author(s)
Mori, M., Yoshida, K., Ishigaki, Y., Matsunaga, T., Nikaido, O., Kameda, K, Kondo, T
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Journal Title
Bioorg. Med. Chem 13
Pages: 2015-2020
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] The involvement of tonoplast proton pumps and Na^+(K^+)/H^+ exchangers in the change of petal color during flower opening of morning glory, Ipomoea tricolor cv. Heavenly Blue2005
Author(s)
Yoshida, K., Kawachi, M., Mori, M., Maeshima, M., Kondo, M., Nishimura, M., Kondo, T
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Journal Title
Plant Cell Physiol 46
Pages: 407-415
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] UV-B protecting effect of a polyacylated anthocyanin, HBA, in flower petals of the blue morning glory, Ipomoea tricolor, cv.Heavenly blue.2005
Author(s)
Mori, M., Yoshida, K., Ishigaki, Y., Matsunaga, T., Nikaido, O., Kameda, K., Kondo, T.
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Journal Title
Bioorg.Med.Chem. 13
Pages: 2015-2020
Related Report
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[Journal Article] The involvement of tonoplast proton pumps and Na+(K+)/H+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv.Heavenly Blue.2005
Author(s)
Yoshida, K., Kawachi, M., Mori, M., Maeshima, M., Kondo, M., Nishimura M., Kondo, T.
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Journal Title
Plant Cell Physiol. (In press)
Related Report
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[Presentation] Role of Tonoplast-localized NHX1 in Blue Morning Glory Petals.2007
Author(s)
Yoshida K, Mikil, N., Katou, K., Okazaki, Y., Uozumi, N., Maeshima, M., Kondo, T.
Organizer
14th International Workshop of Plant Membrane Biology
Place of Presentation
Valencia, SPAIN
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Chemical Studies on Color Development and Variation of Hydrangea macrophylla.2007
Author(s)
Ito, D., Shinkai, Y., Miki, N., Kato, Y., Kondo, T., Yoshida, K.
Organizer
12th Asian Chemical Congress(International Symposium on Natural Products and Medicinal Chemicaty 2007).
Place of Presentation
Kuala Lumpur, MALAYSIA
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Role of Tonoplast-localized NHXl in Blue Morning Glory Petals2007
Author(s)
Yoshida, K., Mikil, N., Katou, K., Okazaki, Y, Uozumi, N., Maeshima, M., Kondo, T
Organizer
14th International Workshop of Plant Membrane Biology
Place of Presentation
Valencia, SPAIN
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Chemical Studies on Color Development and Variation of Hydrangea macrophylla2007
Author(s)
Ito, D., Shinkai, Y., Miki, N., Kato, Y., Kondo, T., Yoshida, K
Organizer
12th Asian Chemical Congress (International Symposium on Natural Products and Medicinal Chemicaty 2007)
Place of Presentation
Kuala Lumpur, MALAYSIA
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Role of Tonoplast-localized NHXI in Blue Morning Glory Petals.2007
Author(s)
Yoshida K., Mikil, N., Katou, K., Okazaki, Y., Uozumi, N., Maeshima, M., Kondo, T.
Organizer
14th International Workshop of Plant Membrane Biology
Place of Presentation
Valencia, SPAIN
Related Report
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[Presentation] Chemical Studies on Color Development and Variation of Hydrangea macrophylla.2007
Author(s)
Ito, D., Shinkai, Y., Miki, N., Kato, Y., Kondo, T., Yoshida, K.
Organizer
12th Asian Chemical Congress (International Symposium on Natural Products and Medicinal Chemicaty 2007).
Place of Presentation
Kuala Lumpur, MALAYSIA
Related Report
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[Presentation] Synthesis of Cyanidin 3-O-β-D-Glucoside.2006
Author(s)
Oyama, K. Nakamura, S., Yamakawa, D., Tokunou, K, Yoshida, K, Kondo, T.
Organizer
ICOB-5 & ISCNP-25 IUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto, JAPAN
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] The Mechanism Underlying Blue Color Development in Tulips.2006
Author(s)
Shoji, K., Miki, N., Nakajima, N., Momonoi, K., Kato, C., Yoshida, K.
Organizer
ICOB-5 & ISCNP-25 IUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto, JAPAN
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Studies on Color Variation of Hydrangea macrophylla on the Basis of Single Cell Analysis.2006
Author(s)
Ito, D., Shinkai, Y., Miki, N., Kato, Y., Kondo, T., Yoshida, K.
Organizer
ICOB-5 & ISCNP-25 IUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto, JAPAN
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Synthesis of Neochlorogenic acid and Other 5-O-acylquinic acid Derivatives Involved in Blue Color Development of Hydtrangea.2006
Author(s)
Yoshida, K., Suzuki, M., Oyama, K., Kato, Y., Kondo, T.
Organizer
XXIII International Conference on Polyphenols.
Place of Presentation
Winnipeg, CANADA
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Synthesis of Cyanidin 3-0-β-D-Glucoside2006
Author(s)
Oyama, K., Nakamura, S., Yamakawa, D., Tokunou, K, Yoshida, K., Kondo, T
Organizer
ICOB-5 & ISCNP-25 IUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto, JAPAN
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] The Mechanism Underlying Blue Color Development in Tulips2006
Author(s)
Shoji, K, Mild, N., Nakajima, N., Momonoi, K., Kato, C., Yoshida, K
Organizer
ICOB-5 & ISCNP-25 IUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto, JAPAN
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Studies on Color Variation of Hydrangea macrophylla on the Basis of Single Cell Analysis2006
Author(s)
Ito, D., Shinkai, Y, Mild, N., Kato, Y., Kondo, T., Yoshida, K
Organizer
ICOB-5 & ISCNP-25 JUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto, JAPAN
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Presentation] Synthesis of Neochlorogenic acid and Other 5-O-acylquinic acid Derivatives Involved in Blue Color Development of Hydtrangea2006
Author(s)
Yoshida, K, Suzuki, M., Oyama, K, Kato, Y., Kondo, T
Organizer
XXIII International Conference on Polyphenols
Place of Presentation
Winnipeg, CANADA
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Synthetic Study on Natural Anthocyanins2006
Author(s)
Kondo, T., Kawaguchi, S., Nakamura, S., Oyama, K., Yoshida, K
Organizer
XXIII International Conference on Polyphenols
Place of Presentation
Wmnipeg, CANADA
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Studies on color change of blue morning glory and pH increase during flower opening.2005
Author(s)
Yoshida, K, Kawachi, M., Mori, M., Maeshima, M., Kondo, M., Nishimura M., Kondo, T.
Organizer
XVII Intyernational Botanic Congress.
Place of Presentation
Wien, AUSTRIA
Description
「研究成果報告書概要(和文)」より
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[Presentation] Studies on blue sepal color development of Hydrangea macrophylla.2005
Author(s)
Kondo, T., Toyama-Kato, Y., Ito, D., Shinkai, Y., Yoshida, K.
Organizer
XVII Intyernational Botanic Congress.
Place of Presentation
Wien, AUSTRIA
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Studies on color change of blue morning glory and pH increase during flower opening2005
Author(s)
Yoshida, K., Kawachi, M., Mori, M., Maeshima, M., Kondo, M., Nishimura, M., Kondo, T
Organizer
XVII Intyernational Botanic Congress
Place of Presentation
Wien, AUSTRIA
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Studies on blue sepal color development of Hydrangea macrophylla2005
Author(s)
Kondo, T., Toyama-Kato, Y, Ito, D., Shinkai, Y., Yoshida, K
Organizer
XVII Intyernational Botanic Congress
Place of Presentation
Wien, AUSTRIA
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Involvement of Na^+/H^+ exchanger in the vacuolar pH increase of epidermal cells of blue morning glory petal.2004
Author(s)
Kawachi, M., Mori, M., Kobae, Y., Maeshima, M., Kondo, T., Yoshida, K.
Organizer
13th International Workshop of Plant Membrane Biology
Place of Presentation
Montperiller, FRANCE
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Involvement of Na^+/H^+ exchanger in the vacuolar pH increase of epidermal cells of blue morning glory petal2004
Author(s)
Kawachi, M., Mori, M., Kobae, Y., Maeshima, M., Kondo, T., Yoshida, K
Organizer
13th International Workshop of Plant Membrane Biology
Place of Presentation
Montperiller, FRANCE
Description
「研究成果報告書概要(欧文)」より
Related Report
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