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Construction of novel systems to analyze a molecular mechanism of shoot gravitropism in Arabidopsis

Research Project

Project/Area Number 16370025
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionNARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Principal Investigator

TASAKA Masao  NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, GRADUATE SCHOOL OF BIOLOGYCAL SCIENCES, PROFESSOR, バイオサイエンス研究科, 教授 (90179680)

Co-Investigator(Kenkyū-buntansha) MORITA Miyo (TERAO Miyo)  NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, GRADUATE SCHOOL OF BIOLOGYCAL SCIENCES, ASSOCIATE PROFESSOR, バイオサイエンス研究科, 助教授 (10314535)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2005: ¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 2004: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsshoot gravitropism / Arabidopsis thaliana / mutants / amyloplast / microarray / 花茎 / オーキシン信号伝達
Research Abstract

Plant can change the growth orientation after sensing the gravity orientation. This response calls gravitropism, which includes four sequential steps ; recognition of gravity orientation, transduction of the physical stimulus to chemical signals, transmission of signals, and differential cell elongation causing organs bending. To elucidate the molecular mechanisms of shoot gravitropism, we have isolated many Arabidopsis mutants, shoot gravitropism (sgr), with reduced or no gravitropic response in inflorescence stems. We have made clear that endoderm cells in inflorescence stems were graviperception sites and amyloplasts in these cells sedimented to the orientation of gravity, which is important for gravity perception. In this project, we analyzed two novel mutants of sgr5 and sgr9, which showed reduced gravitropic response in inflorescence stems. SGR9 encoded a novel protein containing a ring finger domain and the expression of this gene in endoderm cells is important for graviperception. There are two typed endoderm cells in sgr9, one has normal sedimented amyloplasts and the other has abnormal positioning amyloplasts. SGR5 encoded a novel putative transcription factor and functioned in endoderm cells. sgr5 had normal sedimented amyloplasts, however, sgr5sgr9 double mutant showed no gravitropic response and its amyloplasts did not sedimented. These data indicated that these two genes are involved in amyloplasts sedimentation. We isolated some marker genes of gravitropism by microarray analysis, which expressed emphatically after gravity-stimulation in wild type inflorescence stems. One of them was expressed asymmetry after gravity-stimulation, as it was expressed stronger in lower side than in upper side.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (22 results)

All 2006 2005 2004

All Journal Article (22 results)

  • [Journal Article] A C2H2-type zine finger protein, SGR5, is involved in earl events of gravitropism in Arabidopsis inflorescence stems2006

    • Author(s)
      Miyo Terao Morita, et al.
    • Journal Title

      Plant J (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A C2H2-type zinc finger protein, SGR5, is involved in early events of gravitropism in Arabidopsis inflorescence stems2006

    • Author(s)
      Morita T.M, Sakaguchi K., Kiyose S., Taira K., Kato K., Nakamura M., Tasaka M.
    • Journal Title

      Plant Journal (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living graviperceptive cell of the Arabidopsis inflorescence stem2005

    • Author(s)
      Saito C., Morita T.M., Kato T., Tasaka M.
    • Journal Title

      Plant Cell 17

      Pages: 548-558

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts2005

    • Author(s)
      Kotera E., Tasaka M., Shikanai T.
    • Journal Title

      Nature 433

      Pages: 326-330

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis2005

    • Author(s)
      Niihama M., et al.
    • Journal Title

      Current Biology 15

      Pages: 555-560

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Tissue-specificexpression of stabilized SORITARY-ROOT /IAA14 alters lateral root development in Arabidopsis2005

    • Author(s)
      Fukaki H., Nakao Y., Okushima Y., Theolosis A., Tasaka M.
    • Journal Title

      Plant J 44

      Pages: 382-395

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living graviperceptive cell of the Arabidopsis inflorescence stem2005

    • Author(s)
      Saito C., Morita T.M., Kato T., Tasaka M.
    • Journal Title

      The Plant Cell 17

      Pages: 548-558

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis2005

    • Author(s)
      Niihama M., Uemura T., Saito C., Nakano A., Sato M.H., Tasaka M., Morita M.T.
    • Journal Title

      Current Biology 15

      Pages: 555-560

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tissue-specific expression of stabilized SOLITARY-ROOT/IAA14 alters lateral root development in Arabidopsis2005

    • Author(s)
      Fukaki H., Nakao Y., Okushima Y., Theologis A., Tasaka M.
    • Journal Title

      Plant Journal 44

      Pages: 382-395

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living graviperceptive cell of the Arabidopsis inflorescence stem2005

    • Author(s)
      Saito C., Morita T.M., Kato T.Tasaka M.
    • Journal Title

      Plant Cell 1

      Pages: 548-558

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A pentatricopeptide repeat protein is essential for RNA editing it chloroolasts2005

    • Author(s)
      Kotera E., Tasaka M., Shikanai T.
    • Journal Title

      Nature 433

      Pages: 326-330

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Physiological requirements of the nonmevaonate pathway for photo-acclimation in Arabidopsis2005

    • Author(s)
      Hojo M., Tasaka M., Shikanai T.
    • Journal Title

      Plant Biotechnology 22

      Pages: 39-45

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Tissue-specificexpression of stabilized SORITARY-ROOT /IAA14 alters lateral root development in Arabidopsis2005

    • Author(s)
      Fukaki H., Nakao Y., Okushima Y., Theologis A., Tasaka M.
    • Journal Title

      Plant J 44

      Pages: 382-395

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cell cycle progression in the pericycle is not sufficient for SOLITARY ROOT/IAA14-mediated lateral root initiation in Arabidopsis.2005

    • Author(s)
      Vanneste, S., et al.
    • Journal Title

      Plant Cell 17

      Pages: 3035-3050

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The gravitropism defective 2(grv2) mutants of Arabidopsis are deficient in a protein implicated in a protein implicated in endocytosis2004

    • Author(s)
      Mc Cabe R., Kato T., Lukowitz W., Sieber P., Tasaka, M., Somervill C.
    • Journal Title

      Plant Physiology 136

      Pages: 3095-3103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The gravitropism defective 2 (grv2) mutants of Arabidopsis are deficient in a protein implicated in endocytosis2004

    • Author(s)
      McCabe R., Kato T., Lukowitz W., Sieber P., Tasaka M., Somerville C.
    • Journal Title

      Plant Physiology 136

      Pages: 3095-3103

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] PIN-FORMED1 and PINOID regulate boundary formation and cotyledon development in Arabidopsis embryogenesis2004

    • Author(s)
      Furutani M., Vernoux T., Traas J., Kato T., Tasaka M., Aida M.
    • Journal Title

      Development 31

      Pages: 5021-5030

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The gravitropism defective 2 (grv2) mutants of Arabidopsis are deficient in a protein implicated in a protein implicated in endocytosis2004

    • Author(s)
      McCabe R., Kato T., Lukowitz W., Sieber P., Tasaka M., Somerville C.
    • Journal Title

      Plant Physiology 136

      Pages: 3095-3103

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The GURKE Gene Encoding an Acetyl-CoA Carboxylase is Required for Partitioning the Embryo2004

    • Author(s)
      Kajiwara T., Furutani M., Hibara K., Tasaka M.
    • Journal Title

      Plant Cell Physiol 45

      Pages: 1122-1128

    • Related Report
      2004 Annual Research Report
  • [Journal Article] NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON 1 (CUC1) and CUP-SHAPED COTYLEDON 2 (CUC2) : domain analysis of CUC1 and CUC2.2004

    • Author(s)
      Taoka K., Yanagimoto Y., Daimon Y., Hibara K., Aida M., Tasaka M.
    • Journal Title

      The Plant Journal 40

      Pages: 462-473

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cyclic electron flow around photosystem I is essential for photosynthesis2004

    • Author(s)
      Munekage Y., Hashimoto M., Miyake C., Tomizawa K., Endo T., Tasaka M., Shikanai T.
    • Journal Title

      Nature 429

      Pages: 579-582

    • Related Report
      2004 Annual Research Report
  • [Journal Article] PPR motifs of the nucleus-encoded factor, PGR3, function in the selective and distinct step of chloroplast gene expression in Arabidopsis2004

    • Author(s)
      Yamazaki H., Tasaka M., Shikanai T.
    • Journal Title

      The Plant Journal 38

      Pages: 152-163

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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