Construction of novel systems to analyze a molecular mechanism of shoot gravitropism in Arabidopsis
| Project/Area Number |
16370025
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | NARA INSTITUTE OF SCIENCE AND TECHNOLOGY |
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Principal Investigator |
TASAKA Masao NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, GRADUATE SCHOOL OF BIOLOGYCAL SCIENCES, PROFESSOR, バイオサイエンス研究科, 教授 (90179680)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Miyo (TERAO Miyo) NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, GRADUATE SCHOOL OF BIOLOGYCAL SCIENCES, ASSOCIATE PROFESSOR, バイオサイエンス研究科, 助教授 (10314535)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2005: ¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 2004: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | shoot gravitropism / Arabidopsis thaliana / mutants / amyloplast / microarray / 花茎 / オーキシン信号伝達 |
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| Research Abstract |
Plant can change the growth orientation after sensing the gravity orientation. This response calls gravitropism, which includes four sequential steps ; recognition of gravity orientation, transduction of the physical stimulus to chemical signals, transmission of signals, and differential cell elongation causing organs bending. To elucidate the molecular mechanisms of shoot gravitropism, we have isolated many Arabidopsis mutants, shoot gravitropism (sgr), with reduced or no gravitropic response in inflorescence stems. We have made clear that endoderm cells in inflorescence stems were graviperception sites and amyloplasts in these cells sedimented to the orientation of gravity, which is important for gravity perception. In this project, we analyzed two novel mutants of sgr5 and sgr9, which showed reduced gravitropic response in inflorescence stems. SGR9 encoded a novel protein containing a ring finger domain and the expression of this gene in endoderm cells is important for graviperception. There are two typed endoderm cells in sgr9, one has normal sedimented amyloplasts and the other has abnormal positioning amyloplasts. SGR5 encoded a novel putative transcription factor and functioned in endoderm cells. sgr5 had normal sedimented amyloplasts, however, sgr5sgr9 double mutant showed no gravitropic response and its amyloplasts did not sedimented. These data indicated that these two genes are involved in amyloplasts sedimentation. We isolated some marker genes of gravitropism by microarray analysis, which expressed emphatically after gravity-stimulation in wild type inflorescence stems. One of them was expressed asymmetry after gravity-stimulation, as it was expressed stronger in lower side than in upper side.
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Report
(3 results)
Research Products
(22 results)
