| Project/Area Number |
16370028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Meijo University |
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Principal Investigator |
TAKABE Teruhiro Meijo University, Research Institute, Professor, 総合研究所, 教授 (80097766)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yoshito Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (10247679)
HIBINO Takashi Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (70218741)
NAKAMURA Tatsunosuke Niigata University, Pharmacy & Applied Life Science, Professor, 薬学部, 教授 (20114308)
SHIKATA Masamitsu Shimadzu Co., Genomic research, Director, 分析計測事業部ジェノミックリサーチ室, 副主任研究員 (20374126)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2006: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2005: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2004: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | Grovel environment / Food / Salt stress / Glycinebetaine / Genetic engineering / Plants / Halotolerant cyanobacterium / Transport |
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| Research Abstract |
Glycine betaine (betaine) is a important osmoprotectant which accumulates under abiotic stresses. In this projects, we have studied molecular mechanisms of synthesis, transport and accumulation of betaine in plantas and halotolerant cyanobacteria. In this project, we obtained the following results. 1) Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis. 2) Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria. 3) Isolation and functional characterization of betaine transporters from halotolerant cyanobacterium and mangrove. 4) Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter Escherichia coli ChaA. 4) Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity. 5) Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na^+ and pH sensitivity. 6) Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant.
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