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Study on diversity of visual pigments from the viewpoint of the counterion

Research Project

Project/Area Number 16370036
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionOsaka City University (2006)
Kyoto University (2004-2005)

Principal Investigator

TERAKITA Akihisa  Osaka City University, Graduate School of Science, Professor, 大学院理学研究科, 教授 (30212062)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsVisual pigment / Counterion / G protein / Signal transduction / 多様性 / ロドプシン / 対イオン / 視覚 / レチナールシッフ塩基 / G蛋白質共役型受容体 / G蛋白質
Research Abstract

More than 2000 rhodopsins and their related photosensitive proteins (hereby referred to as rhodopsins), and they are divided into roughly 5 classes. This research was aimed at elucidating
1. relationship between molecular evolution of rhodopsins and counterion displacement from G1u181 to Glu113,
2. relationship between molecular properties of rhodopsins that have the Glu181 counterion and their cellular and physiological functions. The research results for three years as follows.
1. We successfully expressed varied rhodopsins in cultured cells and investigated their counterion by using site directed mutant proteins. We found that acquisition of Glull3 counterion resulted in acquisition of a higher G protein activation ability and red-sensitive cone pigment. Biochemical studies suggested that the higher G protein activation ability was due to a specialized conformational change of Glu113-counterion rhodopsin, compared with those of other rhodopsins.
2. We succeeded in expression of Glu181-c … More ounterion rhodopsins, namely parapinopsin, melanopsin and parietopsin. The biochemical, spectroscopic, histochemical and electrophysiological studies revealed the relationship between molecular properties of the rhodopsins and their physiological function as well as evolution of photoreception.
Cheracterization of the amphioxus melanopsin revealed that biochemical and photochemical properties, not just primary structure, of the melanopsin are considerably similar to those of the rhodopsins in the rhabdomeric visual cells of higher invertebrates. Taken together with the fact that the amphioxus melanopsin is expressed in the rhabdomeric photoreceptor cells, the cephalochordate rhabdomeric photoreceptor represents an evolutionary link between the vertebrate photosensitive ganglion cell and the invertebrate rhabdomeric visual cell.
Our molecular biological, histochemical and electorophysiological studies showed a UV pigment functioning in the lamprey pineal is the parapinopsin, which exhibits an absorption maximum at 370 nm. UV light causes a stable photoproduct having an absorption maximum at 515 nm, in the green region. The photoproduct reverts to the original pigment upon visible light absorption, showing photoregeneration of the pigment. The results demonstrated that parapinopsin is the pineal UV pigment having photo-interconvertible two stable states. The bistable nature of the parapinopsin can account for the photorecovery of the pineal UV-sensitivity by background green light in the lamprey.. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (35 results)

All 2007 2006 2005 2004 Other

All Journal Article (34 results) Book (1 results)

  • [Journal Article] 視覚を担うGタンパク質共役型受容体ロドプシン2007

    • Author(s)
      塚本寿夫
    • Journal Title

      ファルマシア (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] G protein coupled receptor rhodopsin in vision.2007

    • Author(s)
      H.Tsukamoto
    • Journal Title

      Farumashia (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural changes in the Schiff base region of squid rhodopsin upon photoisomerization studied by low-temperature FTIR spectroscopy.2006

    • Author(s)
      T.Ota
    • Journal Title

      Biochemistry 45

      Pages: 2845-2851

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Parietal-eye phototransduction components and their potential evolutionary implications.2006

    • Author(s)
      C.-Y.Su
    • Journal Title

      Science 311

      Pages: 1617-1621

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] アゴニストである全トランス型レチナールの結合能を持つロドプシン : 光受容蛋白質の多様性とGPCR研究への展開2006

    • Author(s)
      寺北明久
    • Journal Title

      比較生理生化学 23

      Pages: 3-9

    • NAID

      10025858301

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ロドプシン類の分子進化2006

    • Author(s)
      塚本寿夫
    • Journal Title

      生体の科学 57

      Pages: 500-501

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A rhodopsin exhibiting binding ability to all-trans-retinal as an agonist : Diversity of rhodopsin and possibility as a GPCR model2006

    • Author(s)
      A.Terakita
    • Journal Title

      Hikakuseiriseikagaku 23

      Pages: 3-9

    • NAID

      10025858301

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular evolution of rhodopsins.2006

    • Author(s)
      H.Tsukamoto
    • Journal Title

      Seitainokagaku 57

      Pages: 500-501

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural changes in the Schiff base region of squid rhodopsin upon photoisomerization studied by low-2006

    • Author(s)
      T.Ota
    • Journal Title

      Biochemistry 45

      Pages: 2845-2851

    • Related Report
      2005 Annual Research Report
  • [Journal Article] アゴニストである全トランス型レチナールの結合能を持つロドプシン:光受容蛋白質の多様性とGPCR研究への展開2006

    • Author(s)
      寺北明久
    • Journal Title

      比較生理生化学 23

      Pages: 3-9

    • NAID

      10025858301

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A rhodopsin exhibiting binding ability to agonist all-trans-r et al.2005

    • Author(s)
      H.Tsukamoto
    • Journal Title

      Proc Natl. Acad. Sci. USA 102

      Pages: 6303-6308

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] FTIR Studies of the Photoactivation Processes in Squid Retinochrome.2005

    • Author(s)
      Y.Furutani
    • Journal Title

      Biochemistry 44

      Pages: 7988-7997

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cephalochordate melanopsin : Evolutionary linkage between invertebrate visual cells and vertebrate photosensitive retinal ganglion cells.2005

    • Author(s)
      M.Koyanagi
    • Journal Title

      Curr. Biol. 15

      Pages: 1065-1069

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The opsins.2005

    • Author(s)
      A.Terakita
    • Journal Title

      Genome Biology 6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ロドプシンの分子設計とその多様性2005

    • Author(s)
      寺北明久
    • Journal Title

      生物物理 45

      Pages: 302-307

    • NAID

      10016895493

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] A rhodopsin exhibiting binding ability to agonist all-trans-r et al.2005

    • Author(s)
      H.Tsukamoto
    • Journal Title

      Proc Natl.Acad.Sci.USA 102

      Pages: 6303-6308

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cephalochordate melanopsin : Evolutionary linkage between invertebrate visual cells and vertebrate photosensitive retinal ganglion cells.2005

    • Author(s)
      M.Koyanagi
    • Journal Title

      Curr.Biol. 15

      Pages: 1065-1069

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular architecture of rhodopsin and its diversification.2005

    • Author(s)
      A.Terakita
    • Journal Title

      Seibutubuturi 45

      Pages: 302-307

    • NAID

      10016895493

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] FTIR Studies of the Photoactivation Processes in Squid Retinochrome2005

    • Author(s)
      Y.Furutani
    • Journal Title

      Biochemistry 44

      Pages: 7988-7997

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cephalochordate melanopsin : Evolutionary linkage between invertebrate visual cells and vertebrate photosensitive2005

    • Author(s)
      M.Koyanagi
    • Journal Title

      Current Biology 15

      Pages: 1065-1069

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The Opsins2005

    • Author(s)
      Akihisa Terakita
    • Journal Title

      Genome Biology 6(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Counterion displacement in the molecular evolution of the rhodopsin family.2004

    • Author(s)
      A.Terakita
    • Journal Title

      Nat. Struct. Mol. Biol. 11

      Pages: 284-289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bistable UV pigment in the lamprey pineal.2004

    • Author(s)
      M.Koyanagi
    • Journal Title

      Proc. Natl. Acad. Sci. USA 101

      Pages: 6687-6691

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A novel constitutively active mutation in the second cytoplasmic loop of metabotropic glutamate receptor.2004

    • Author(s)
      T.Yamashita
    • Journal Title

      J. Neurochem. 91

      Pages: 484-492

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 脊椎動物の視覚と視物質の分子進化2004

    • Author(s)
      寺北明久
    • Journal Title

      生体の科学 55

      Pages: 211-216

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] ロドプシン類の分子進化と多様性2004

    • Author(s)
      寺北明久
    • Journal Title

      比較生理生化学 21

      Pages: 22-29

    • NAID

      10025857289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Counterion displacement in the molecular evolution of the rhodopsin family.2004

    • Author(s)
      A.Terakita
    • Journal Title

      Nat.Struct.Mol Biol. 11

      Pages: 284-289

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bistable UV pigment in the lamprey pineal.2004

    • Author(s)
      M.Koyanagi
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 6687-6691

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A novel constitutively active mutation in the second cytoplasmic loop of metabotropic glutamate receptor.2004

    • Author(s)
      T.Yamashita
    • Journal Title

      J.Neurochem. 91

      Pages: 484-492

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular evolution and diversity of rhodopsins2004

    • Author(s)
      A.Terakita
    • Journal Title

      Hikakuseiriseikagaku 21

      Pages: 22-29

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Vertebrate vision and visual pigment evolution.2004

    • Author(s)
      A.Terakita
    • Journal Title

      Seitainokagaku 55

      Pages: 211-216

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bistable UV pigment in the lamprey pineal2004

    • Author(s)
      Mitsumasa Koyanagi
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 6687-6691

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel constitutively active mutation in the second cytoplasmic loop of metabotropic glutamate receptor2004

    • Author(s)
      Takahiro Yamashita
    • Journal Title

      J.Neurochem. 91

      Pages: 484-492

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Primary process in vision

    • Author(s)
      A.Terakita
    • Journal Title

      21-seiki-no-doubutsukagaku, Kankaku-to-rizumu (Baifukan) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 21世紀の動物科学 感覚とリズム(分担執筆 : 視覚の初期過程)

    • Author(s)
      寺北明久
    • Publisher
      培風館(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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