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Regulation of cell function by protein dephosphorylation

Research Project

Project/Area Number 16370055
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionMiyagi Cancer Center Research Institute (2006)
Hokkaido University (2004-2005)

Principal Investigator

SHIMA Hiroshi  Miyagi Cancer Center Research Institute, Division of Cancer Chemotherapy, Head, 薬物療法学部, 部長 (10196462)

Co-Investigator(Kenkyū-buntansha) TANUMA Nobuhiro  Miyagi Cancer Center Research Institute, Division of Cancer Chemotherapy, Staff, 薬物療法学部, 研究員 (40333645)
NOMURA Miyuki  Miyagi Cancer Center Research Institute, Division of Cancer Chemotherapy, Staff, 薬物療法学部, 技官 (40390893)
KIKUCHI Kunimi  Hokkaido University Institute for Genetic Medicine, Divisiojn of Biochemical Oncology and Immunology, Professor, 遺伝子病制御研究所, 教授 (20006117)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2004: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsTCPTP / MKP7 / LDP4 / PP2A / I-1^<PP2A> / pp32 / I-2^<PP2A> / SET / PP1 / Aurora kinase / T-cell PTP / PTP1B / manzamenone / 阻害剤 / ビメンチン / 中心体 / プロテインホスファターゼ / MAPK / MAPKホスファターゼ / ユビキチン / LDP / 浸透圧刺激 / siRNA
Research Abstract

Development of novel inhibitors of PTP
Manzamenone was identified as a novel inhibitor of T-Cell Protein Tyrosine Phosphatase
Regulation of signal transduction by protein phosphatases
(1)Phosphorylation of Ser-446 determined stability of MKP-7.
(2)Low-molecular-mass dual-specificity phosphaase (LDP-3) enhanced activation of JNK and p38.
Regulation of transcription and translation by protein phosphatases
(1)Theoncoprotein I-2^<PP2A>/SET negatively regulated the MEK/ERK pathway and cell proliferation.
(2)pp32/I-1^<PP2A> negatively regulated the Raf/MEK/ERK pathway.
Regulation of cell morphology by protein phosphatases
(1)Isoform specific function of PP1 catalytic subunits was observed by using siRNA.
(2)Vimentin-Ser82 was identified as a memory phosphorylation site in astrocytes.
Regulation of cell division by protein phosphatases
PP1 regulated Aurora-A activity by dephosphorylatiohn.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (30 results)

All 2007 2006 2005 2004 2003

All Journal Article (28 results) Book (2 results)

  • [Journal Article] Characterization of novel low-molecular-mass dual specificity phosphatase-4 (LDP-4) expressed in brain2007

    • Author(s)
      Kentaro Takagaki
    • Journal Title

      Mol Cellular Biochem 296

      Pages: 177-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of novel low-molecular-mass dual specificity phosphatase-4 (LDP-4) expressed in brain2007

    • Author(s)
      Kentaro Takagaki
    • Journal Title

      Molecular and Cellular Biochemistry 296

      Pages: 177-184

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Manzamenones Inhibits T-Cell Protein Tyrosine Phosphatase2006

    • Author(s)
      Yusuke Wakuda
    • Journal Title

      Marine Drugs 4

      Pages: 9-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Vimentin-Ser82 as a memory phosphorylation site in astrocytes2006

    • Author(s)
      Takashi Oguri
    • Journal Title

      Genes to Cells 11

      Pages: 531-540

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Phospho-regulation of human protein kinae A Aurora-A : analysis using anti-phospho-Thr 288 monoclonal antibodies2006

    • Author(s)
      Sayaka Ohashi
    • Journal Title

      Oncogene 25

      Pages: 7691-7702

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Vimentin-Ser82 as a memory phosphorylation site in astrocytes.2006

    • Author(s)
      Oguri T, Inoko A, Shima H, Izawa I, Arimura N, Yamaguchi T, Inagaki N, Kaibuchi K, Kikuchi K, Inagaki M.
    • Journal Title

      Genes Cells 11

      Pages: 531-540

    • NAID

      120000961744

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Manzamenones inhibit T-Cell Protein Tyrosine Phosphatase2006

    • Author(s)
      Wakuda Y, Kubota T, Shima H, Okada T, Mitsuhashi S, Aoki N, Kikuchi K, Kobayashi J.
    • Journal Title

      Mar.Drugs 4

      Pages: 9-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of a novel low-molecular- mass dual specificity phosphatase-4(LDP-4) expressed in brain2006

    • Author(s)
      Takagaki K, Shima H, Tanuma N, Nomura M, Satoh T, Watanabe M, Kikuchi K.
    • Journal Title

      Mol Cell Biochem 296

      Pages: 177-184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phospho-regulation of human protein kinae A Aurora-A : analysis using anti-hospho-Thr 288 monoclonal antibodies2006

    • Author(s)
      Sayaka Ohashi
    • Journal Title

      Oncogene 25

      Pages: 7691-7702

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The oncoprotein I-2PP2A/SET negatively regulates the MEK/ERK pathway and cell proliferation.2005

    • Author(s)
      Chikako Fukukawa
    • Journal Title

      Int J Oncol 26

      Pages: 751-756

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Protein phosphatase type 2A, PP2A, is involved in degradation of gp 130.2005

    • Author(s)
      Shinya Mitsuhashi
    • Journal Title

      Mol Cell Biochem 269

      Pages: 183-187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] pp32/I-1 PP2A negatively regulates the Raf/MEK/ERK pathway.2005

    • Author(s)
      Chikako Fukukawa
    • Journal Title

      Cancer Lett 226

      Pages: 155-160

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phosphorylation of Ser-446 determines stability of MKP-7.2005

    • Author(s)
      Chiaki Katagiri
    • Journal Title

      J Biol Chem 280

      Pages: 14716-14722

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] pp32/I-1^<PP2A> negatively regulates the Raf/MEK/ERK pathway.2005

    • Author(s)
      Fukukawa C, Tanuma N, Okada T, Kikuchi K, Shima H.
    • Journal Title

      Cancer Lett 226

      Pages: 155-160

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phosphorylation of Ser-446 determines stability of MKP-7.2005

    • Author(s)
      Katagiri C, Masuda K, Urano T, Yamashita K, Kikuchi K, Shima H.
    • Journal Title

      J Biol Chem 280

      Pages: 14716-14722

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phospho-regulation of human protein kinase Aurora-A : analysis using anti-phopho-Thr288 monoclonal antibodies.2005

    • Author(s)
      Ohashi S, Sakashita G, Ban R, Nagasawa M, Matsuzaki H, Murata Y, Taniguchi H, Shima H, Furukawa K, Urano T.
    • Journal Title

      Oncogene 25

      Pages: 7691-7702

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Protein phosphatase type 2A, PP2A, is involved in degradation of gp130.2005

    • Author(s)
      Shinya Mitsuhashi
    • Journal Title

      Mol Cell Biochem 269

      Pages: 183-187

    • Related Report
      2004 Annual Research Report
  • [Journal Article] pp32/I-1PP2A negatively regulates the Raf/MEK/ERK pathway.2005

    • Author(s)
      Chikako Fukukawa
    • Journal Title

      Cancer Lett (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of a novel low-molecular-mass dual-specificity phosphaase 3(LDP-3) that enhances activation of JNK and p38.2004

    • Author(s)
      Kentaro Takagaki
    • Journal Title

      Biochem J 383

      Pages: 447-455

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of isoform specific function of PP1 catalytic subunits in mammalian cells using siRNA.2004

    • Author(s)
      Tadashi Okada
    • Journal Title

      Int J Oncol 25

      Pages: 1383-1388

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nagelamides A-H, new dimeric bromopyrrole alkaloids from marine sponge agelas species.2004

    • Author(s)
      Tadashi Endo
    • Journal Title

      J Nat Prod 68

      Pages: 1262-1267

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Characterization of a novel low-molecular-mass dual-specificity phosphaase 3(LDP-3) that enhances activation of JNK and p38.2004

    • Author(s)
      Takagaki K, Satoh T, Tanuma N, Masuda K, Takekawa M, Shima H, Kikuchi K.
    • Journal Title

      Biochem J 383

      Pages: 447-455

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of isoform specific function of PP1 catalytic subunits in mammalian cells using siRNA.2004

    • Author(s)
      Okada T, Fujii T, Tanuma N, Mitsuhashi S, Urano T, Araki Y, Shima H, Kikuchi K.
    • Journal Title

      Int J Oncol 25

      Pages: 1383-1388

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nagelamides A-H, new dimeric bromopyrrole alkaloids from marine sponge agelas species.2004

    • Author(s)
      Endo T, Tsuda M, Okada T, Mitsuhashi S, Shima H, Kikuchi K, Mikami Y, Fromont J, Kobayashi J.
    • Journal Title

      J Nat Prod 68

      Pages: 1262-1267

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The oncoprotein I-2^<PP2A>/SET negatively regulates the MEK/ERK pathway and cell proliferation.2004

    • Author(s)
      Fukukawa C, Shima H, Tanuma N, Okada T, Kato N, Adachi Y, Kikuchi K.
    • Journal Title

      Int J Oncol 26

      Pages: 751-756

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Protein phosphatase type 2A, PP2A, is involved in degradation of gp130.2004

    • Author(s)
      Mitsuhashi S, Shima H, Tanuma N, Sasa S, Onoe K, Ubukata M, Kikuchi K.
    • Journal Title

      Mol Cell Biochem 269

      Pages: 183-187

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of a novel low-molecular-mass dual-specificity phosphaase 3(LDP-3) that enhances activation of JNK and P38.2004

    • Author(s)
      Kentaro Takagaki
    • Journal Title

      Biochem J 383

      Pages: 447-455

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Analysis of isoform specific function of PP1 catalytic subunits in mammalial cells using siRNA.2004

    • Author(s)
      Tadashi Okada
    • Journal Title

      Int J Oncol 25

      Pages: 1383-1388

    • Related Report
      2004 Annual Research Report
  • [Book] 生化学2005

    • Author(s)
      島礼
    • Total Pages
      8
    • Publisher
      生化学会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 細胞工学2003

    • Author(s)
      島礼
    • Total Pages
      4
    • Publisher
      秀潤社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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