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Unusual Structure and Function of Active Intermediate of Myosin Realized during Sliding Movement : A Possibility for Energy Storage State

Research Project

Project/Area Number 16370070
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

KATAYAMA Eisaku  The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (50111505)

Co-Investigator(Kenkyū-buntansha) UYEDA Taro  The University of Tokyo, Gene Function Research Center, National Institute for Advanced Industrial Science and Technology, Deputy Director Resesrcher, ジーンファンクション研究センター, 副センター長 (90356551)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2005: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2004: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsSliding Movement / Actomyosin / Quick-Freeze Electron Microscopy / 3-D Reconstruction / Differential structural Analysis / Myosin-V / High Performance Probe / Analysis of Protein Dynamics / 急速凍結フリーズ・レプリカ法 / 3次元構造解析 / アクトミオシン滑り運動 / コンフォメーション変化 / レバーアーム首振り説 / 電子顕微鏡 / 活性複合体 / 硬直複合体
Research Abstract

As a means to investigate the 3-D structural changes of sliding actomyosin, we have developed a "differential pattern-recognition method" to quantitatively compare the surface profiles of the protein assembly instantaneously captured. by quick-freeze deep-etch replication, with those of the artificial images simulated from the atomic models of the target. We discovered that actin-attached myosin head during sliding takes a configuration kinked to the opposite direction from widely believed ATP-bound form. Since we also found the presence of particles in the same configuration but with different attachment angle to actin, we confirmed the significance of that novel structure, and proposed a new scheme of crossbridge movement, that is quite different from the conventional one, but comprehensively accounts for the presence of all the observed structures. We also analyzed the structures of actin-attached myosin-V motor-domain with long lever-arm moiety, during processive walking movement a … More long actin-filament, by a similar means. The trail head showed a rigor-like pattern as postulated in a preceding study, but the images of the lead heads matched by far better with our novel structure than ATP-bound form postulated in the above study, suggesting that the structural change we found in myosin-II might be universally applicable to unconventional myosin species. We are also developing a novel multifunctional microscopic probe to indicate the localization of specific sites or subdomains in the protein assembly, which might enable us to follow the dynamic behavior of given molecules even within intracellular environments. We successfully fused the probe construct to myosin head and attempted to visualize the movement of the probe using the world best-rated atomic force microscope with highest temporal and spatial resolutions. However, the movement was too fast and subtle and the material was not well-immobilized onto mica surface. We would retry after enhancing the adsorption and introducing multiple probes in a molecule. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (31 results)

All 2006 2005 2004

All Journal Article (29 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] A Procedure to Analyze Surface Profiles of the Protein Molecules Visualized by Quick-freeze Deep-etch Replica Electron Microscopy2006

    • Author(s)
      Kimori Y, Oguchi Y, Ichise N, Baba N, Katayama E.
    • Journal Title

      Ultramicroscopy In press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cargo binding makes a wild-type single-headed myosin-VI move processively.2006

    • Author(s)
      Iwaki M, Tanaka H, Iwane AH, Katayama E, Ikebe M, Yanagida T.
    • Journal Title

      Biophysical Journal 90

      Pages: 3643-3652

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Shigella Spa33 is an essential C-ring component of type III secretion machinery.2006

    • Author(s)
      Morita-Ishihara T, Ogawa M, Sagara H, Yoshida M, Katayama E, Sasakawa C.
    • Journal Title

      Journal of Biological Chemistry 281

      Pages: 599-607

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A Procedure to Analyze Surface Profiles of the Protein Molecules Visualized by Quick-freeze Deep-etch Replica Electron Microscopy2006

    • Author(s)
      Kimori Y, Oguchi Y, lchise N, Baba, N, Katayama E.
    • Journal Title

      Ultramicroscopy. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cargo binding makes a wild-type single-headed myosin-VI move processively.2006

    • Author(s)
      lwaki M, Tanaka H, lwane AH, Katayama E, lkebe M, Yanagida T.
    • Journal Title

      Biophysical Journal. 90 (10)

      Pages: 3643-3652

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Shigella Spa33 is an essential C-ring component of type III secretion machinery.2006

    • Author(s)
      Morita-lshihara T, Ogawa M, Sagara H, Yoshida M, Katayama E, Sasakawa C.
    • Journal Title

      Journal of Biological Chemistry 281 (1)

      Pages: 599-607

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cargo binding makes a wild-type single-headed myosin-VI move processivlty.2006

    • Author(s)
      Iwaki M, Tanaka H, Iwane AH, Katayama E, Ikebe M, Yanaida T
    • Journal Title

      Biophysical Journal Epub ahead of print(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Shigella Apa33 is an essential C-ring component of type iii secretion machinery.2006

    • Author(s)
      Morita-Ishihara T, Ogawa M, Sagara H, Yoshida M, Katayama E, Sasakawa C
    • Journal Title

      Journal of Biological Chemistry 281(1)

      Pages: 599-607

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cooperative structural change of actin filaments interacting with activated myosin motor domain, detected with copolymers of pyrene-labeled actin and acto-S1 chimera protein.2005

    • Author(s)
      Siddique MS, Mogami G, Miyazaki T, Katayama E, Uyeda TQ, Suzuki M.
    • Journal Title

      Biochemical and Biophysical Research Communications 33

      Pages: 1185-1191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] High-performance purification of gelsoin from plasma using anion-exchange porous hollow-fiber membrane.2005

    • Author(s)
      Hagiwara K, Yonedu S, Saito K, Shiraishi T, Sugo T, Tojyo T, Katayama E.
    • Journal Title

      Journal of Chromatography B Analytical Technology and Biomedical Life Sciences 821

      Pages: 153-158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evidence against essential roles for subdomain 1 of actin in actomyosin sliding movements.2005

    • Author(s)
      Siddique MS, Miyazaki T, Katayama E, Uyeda TQ, Suzuki M.
    • Journal Title

      Biochemical and Biophysical Research Communications 332

      Pages: 474-481

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cooperative structural change of actin filaments interacting with activated myosin motor domain, detected with copolymers of pyrene-labeled actin and acto-S1 chimera protein.2005

    • Author(s)
      Siddique MS, Mogami G, Miyazaki T, Katayama E, Uyeda TQ, Suzuki M.
    • Journal Title

      Biochemical and Biophysical Research Communications 33 (4)

      Pages: 1185-1191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] High-performance purification of gelsolin from plasma using anion-exchange porous hollow-fiber membrane.2005

    • Author(s)
      Haglwara K, Yonedu S, Saito K, Shiraishi T, Sugo T, Tojyo T, Katayama E.
    • Journal Title

      Journal of Chromatography B Analytical Technology, Biomedical and Life Sciences 821 (2)

      Pages: 153-158

    • NAID

      10016978096

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evidence against essential roles for subdomain 1 of actin in actomyosin sliding movements.2005

    • Author(s)
      Siddique MS, Miyazaki T, Katayama E, Uyeda TQ, Suzuki M.
    • Journal Title

      Biochemical and Biophysical Research Communications 332 (2)

      Pages: 474-481

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cooperative structural change of actin filaments interacting with activated myosin motor domain, detected with copolymers of pyrene-labeled actin and acto-S1 chimera protein.2005

    • Author(s)
      Siddique MA, Mogami G, Miyazaki T, Katayama E, Ueda TQ, Suzuki M
    • Journal Title

      Biochemical and Biophysical Research Communications 337(4)

      Pages: 1185-1191

    • Related Report
      2005 Annual Research Report
  • [Journal Article] High-performance purification of gelsolin from plasma using anion-exchange porous hollow-fiber membrane.2005

    • Author(s)
      Hagiwara K, Yonedu S, Saito K, Shiraishi T, Sugo T, Tojyo T, Katayama E
    • Journal Title

      Journal of Chromatography B Analytical Technology and Biomedical Life Science 821(2)

      Pages: 153-158

    • NAID

      10016978096

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Evidence against essential roles for subdomain 1 of actin in actomyosin sliding movements.2005

    • Author(s)
      Siddique MS, Miyazaki T, Katayama E, Uyeda TQ, Suzuki M
    • Journal Title

      Biochemical and Biophysical Research Communications 332(2)

      Pages: 474-481

    • Related Report
      2005 Annual Research Report
  • [Journal Article] αB-crystallin-coated MAPs-microtubule resists nocodazole- and calcium-induced disassembly.2004

    • Author(s)
      Fujita Y, Ohto E, Katayama E, Atomi Y.
    • Journal Title

      Journal of Cell Science 117

      Pages: 1719-26

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tri-dimensional morphometrical analyses of astrocytic processes with the high voltage electron microscopy of thick Golgi preparations2004

    • Author(s)
      Hama K, Arii T, Katayama E, Marton M, Ellisman MH.
    • Journal Title

      Journal of Neurocytology 33

      Pages: 277-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction of myosin/ADP/fluorometal complexes with fluorescent probes and direct observation using quick-freeze deep-etch electron microscopy.2004

    • Author(s)
      Maruta S, Uyehara Y, Aihara T, Katayama E.
    • Journal Title

      Journal of Biochemistry 136

      Pages: 57-64

    • NAID

      10016200785

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 急速凍結ディープエッチ・レプリカ法 : その特長と最近の企てについて2004

    • Author(s)
      片山 栄作
    • Journal Title

      顕微鏡 39

      Pages: 133-136

    • NAID

      10013445089

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] αB-crystallin-coated MAPs-microtubule resists nocodazole-and calcium-induced disassembly.2004

    • Author(s)
      Fujita Y, Ohto E, Katayama E, Atomi Y.
    • Journal Title

      Journal of Cell Science 117 (9)

      Pages: 1719-1726

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tri-dimensional morphometrical analyses of astrocytic processes with the high voltage electron microscopy of thick Golgi preparations2004

    • Author(s)
      Hama K, Arii T, Katayama E, Marton M, Ellisman MH.
    • Journal Title

      Journal of Neurocytology 33 (3)

      Pages: 277-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction of myosin/ADP/fluorometal complexes with fluorescent probes and direct observation using quick-freeze deep-etch electron microscopy.2004

    • Author(s)
      Maruta S, Uyehara Y, Aihara T, Katayama E.
    • Journal Title

      Journal of Biochemistry 136 (1)

      Pages: 57-64

    • NAID

      10016200785

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quick-freeze deep-etch replication : Its advantageous features and recent attempts. (in Japanese)2004

    • Author(s)
      Katayama E, Baba N.
    • Journal Title

      Kenbikyo 39 (2)

      Pages: 133-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] αB-crystallin-coated MAPs-microtubule resists nocodazole- and calcium-induced disassembly.2004

    • Author(s)
      Fujita Y., Katayama E.
    • Journal Title

      Journal of Cell Science 117

      Pages: 1719-1726

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Three dimensional morphometrical analyses of astrocytic processes with the high voltage electron microscopy of thick Golgi2004

    • Author(s)
      Hama K., Katayama E.
    • Journal Title

      Journal of Neurocytology 33

      Pages: 277-285

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interaction of myosin/ADP/fluorometal complexes_with fluorescent probes and direct observation using quick-freeze deep-etch electron microscopy.2004

    • Author(s)
      Maruta S., Katayama E.
    • Journal Title

      Journal of Biochemistry 136

      Pages: 57-64

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 急速凍結ディープエッチ・レプリカ法:その特長と最近の企てについて2004

    • Author(s)
      片山 栄作
    • Journal Title

      顕微鏡 39

      Pages: 133-136

    • NAID

      10013445089

    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 顕微鏡解析用プローブ及び顕微鏡解析方法2005

    • Inventor(s)
      片山栄作
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Number
      2005-168887
    • Filing Date
      2005-06-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 顕微鏡解析用プロープ及び顕微鏡解析方法2005

    • Inventor(s)
      片山栄作
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Number
      2005-168887
    • Filing Date
      2005-06-08
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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