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Structure-function relationships of a yeast putative stretch-activated calcium channel

Research Project

Project/Area Number 16370072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTokyo Gakugei University

Principal Investigator

IIDA Hidetoshi  Tokyo Gakugei University, Department of Education, Professor, 教育学部, 教授 (70124435)

Co-Investigator(Kenkyū-buntansha) IIDA Kazuko  Tokyo Metropolitan Organization for Medical Research, The Tokyo Metropolitan Institute of Medical Science, Senior Researcher, 東京都臨床医学総合研究所, 主任研究員 (40151229)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2004: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsStretch-activated calcium channel / Calcium channel / Signal transduction / Saccharomyces cerevisiae / Arabidopsis thaliana / Calcium / Mid1 / Cch1 / Mca1 / 機械刺激 / イネ / Spa2 / OsTpc1 / 電位作動型カルシウムチャネル / 伸展活性化Ca^<2+>チャネル / 機械受容チャネル / Ca^<2+>チャネル / 電立依存性Ca^<2+>チャネル
Research Abstract

1) The amino acid residue Phe^<356> of the putative stretch-activated Ca^<2+> channel Mid1 was determined to be important for cell viability in yeast. Systematic substitution of various amino acids for Phe^<355> revealed that hydrophobicity of this residue is critical.
2) The Mid1 protein was found to be localized to the plasma membrane and endoplasmic reticulum membrane. Mid1 forms a oligomer via disulfide bonding.
3) A multicopy suppressor of the mid1 mutation was isolated and characterized. This multicopy suppressor is an N-terminally truncated form of the Spa2 protein important for polarized morphgenesis.
4) A gene encoding Cch1, which has been genetically suggested to function with Mid1 as a Ca^<2+> channel, was cloned. We have confirmed that Mid1 and Cch1 really cooperate to function as a Ca^<2+> channel.
5) Rice OsTpc1 that is homologous to yeast Cch1 was found to be sensitive to blockers of L-type voltage-gated calcium channels. In addition, The Arabidopsis Mca1 protein, which can complement the lethality of the yeast mid1 mutant, was isolated and characterized. Mca1 is crucial for Ca^<2+> influx and touch sensing in primary roots.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (30 results)

All 2007 2005 2004

All Journal Article (27 results) Book (3 results)

  • [Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+> influx and touch sensing in roots2007

    • Author(s)
      Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka, T., Iida.H.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 3639-3644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+> influx and touch sensing in roots.2007

    • Author(s)
      Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka, T., Iida H.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 104

      Pages: 3639-3644

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+>influx and touch sensing in roots2007

    • Author(s)
      Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka,T., Iida,H.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 104

      Pages: 3639-3644

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The polarized morphogenesis regulator Spa2 is required for the function of the putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae2005

    • Author(s)
      Noma, S., Iida, K., Iida, H.
    • Journal Title

      Eukaryot. Cell 4

      Pages: 1353-1363

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evidence for the plasma membrane localization of a putative voltage-dependent Ca^<2+> channel, OsTPC1, in rice.2005

    • Author(s)
      Hashimoto, K., Saito, M., Iida, H., Matsuoka, H
    • Journal Title

      Plant Biotechnol. 22

      Pages: 235-239

    • NAID

      10028053758

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of functional domains of Mid1, a stretch-activated channel component, necessary for localization to the plasma membrane and Ca^<2+> permeation2005

    • Author(s)
      Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida, H., Sokabe, M.
    • Journal Title

      Exp. Cell Res. 311

      Pages: 84-95

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of functional domains of Mid1, stretch-activated channel component, necessary for localization to the plasma membrane and Ca^<2+> permeation.2005

    • Author(s)
      Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida H., Sokabe, M.
    • Journal Title

      Exp.Cell Res. 311

      Pages: 84-95

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Polarize morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca^<2+>-permeable channel component Mid1 in Saccharomyces cerevisiae.2005

    • Author(s)
      Noma, S., Iida, K., Iida.H.
    • Journal Title

      Eukaryot.Cell 4

      Pages: 1353-1363

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evidence for the plasma membrane localization of a putative voltage-dependent Ca^<2+> channel, in rice.2005

    • Author(s)
      Hashimoto, K., Saito, M., Iida H., Matsuoka, H.
    • Journal Title

      Plant Biotechnol. 22

      Pages: 235-239

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae2005

    • Author(s)
      Noma, S., Iida, K., Iida, H.
    • Journal Title

      Eukaryotic Cell 4

      Pages: 1353-1363

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Evidence for the plasma membrane localization of a putative voltage-dependent Ca2+ channel, OsTPC1, in rice2005

    • Author(s)
      Hashimoto, K., Saito, M., Iida, H., Matsuoka, H.
    • Journal Title

      Plant Biotechnology 22

      Pages: 235-239

    • NAID

      10028053758

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of functional domains of Mid1, a stretch-activated channel component, necessary for localization to the plasma membrane and Ca2+ permeation2005

    • Author(s)
      Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida, H., Sokabe, M.
    • Journal Title

      Experimental Cell Research 311

      Pages: 84-95

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phe356 in the yeast Ca2+ channel component Mid1 is a key residue for viability after exposeure to □-factor2004

    • Author(s)
      Tada, T., Ohmori, M., Iida, H.
    • Journal Title

      Biochem. Biophs. Res. Commun 313

      Pages: 752-757

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca2+ channel component Mid12004

    • Author(s)
      Yoshimura, H., Tada, T., Iida, H
    • Journal Title

      Exp. Cell Res. 293

      Pages: 185-195

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional analysis of a rice putative voltage-dependent Ca2+ channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH12004

    • Author(s)
      Hashimoto K., Saito, M., Matsuoka, H., Iida, K., Iida, H.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 496-500

    • NAID

      10012852684

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A mechanosensitive anion channel in Arabidopsis thaliana mesophyll cells2004

    • Author(s)
      Qi, Z., Kishigami, A., Nakagawa, Y., Iida, H., Sokabe, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1704-1708

    • NAID

      10014020982

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning in yeast by in vivo homologous recombination of the yeast putative □1 subunit of the voltage-gated calcium channel2004

    • Author(s)
      Iida, K., Tada, T., Iida, H.
    • Journal Title

      FEBS Lett. 576

      Pages: 291-296

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca^<2+> channel component Mid1.2004

    • Author(s)
      Yoshimura, H., Tada, T., Iida, H.
    • Journal Title

      Exp.Cell Res. 293

      Pages: 185-195

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phe^<356> in the yeast Ca^<2+> channel comopnent Mid1 is a key residue for viability after exposure to α-factor.2004

    • Author(s)
      Tada, T., Ohmori, M., Iida H.
    • Journal Title

      Biochem.Biophys.Res.Commun. 313

      Pages: 752-757

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional analysis of a rice putative voltage-dependent Ca^<2+> channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1.2004

    • Author(s)
      Hashimoto, K., Saito, M., Matsuoka, H., Iida, K., Iida H.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 496-500

    • NAID

      10012852684

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning in yeast by in vivo homologous recombination of the yeast putative α1 subunit of the voltage-gated calcium channel.2004

    • Author(s)
      Iida, K., Tada, T., Iida H.
    • Journal Title

      FEBS Lett. 576

      Pages: 1704-1708

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A mechanosensitive anion channel in Arabidopsis thaliana mesophyll.cells.2004

    • Author(s)
      Qi, Z., Kishigami, A., Nakagawa, Y., Iida, H., Sokabe, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1704-1708

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phe356 in the yeast Ca^<2+> channel component Mid1 is a key residue for viability after exposure to α-factor2004

    • Author(s)
      Tada, T., Ohmori, M., Iida, H.
    • Journal Title

      J.Biol.Chem. 313

      Pages: 752-757

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca^<2+> channel component Mid12004

    • Author(s)
      Yoshimura, H., Tada, T., Iida, H.
    • Journal Title

      Exp.Cell Res 293

      Pages: 185-195

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional analysis of a rice putative voltage-dependent Ca^<2+> channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1.2004

    • Author(s)
      Hashimoto, K., Saito, M., Matsuoka, H., Iida, K., Iida, H.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 496-500

    • NAID

      10012852684

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular cloning in yeast by in vivo homologous recombination of the yeast putative α1 subunit of the voltage-gated calcium channel.2004

    • Author(s)
      Iida, K., Tada, T., Iida, H.
    • Journal Title

      FEBS Lett. 576

      Pages: 291-296

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A mechanosensitive anion channel in Arabidopsis thaliana mesophyll cells.2004

    • Author(s)
      Qi, Z., Kishigami, A., Nakagawa, Y., Iida, H., Sokabe, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1704-1708

    • NAID

      10014020982

    • Related Report
      2004 Annual Research Report
  • [Book] 植物の環境応答と形態形成のクロストーク2004

    • Author(s)
      飯田秀利
    • Total Pages
      9
    • Related Report
      2004 Annual Research Report
  • [Book] カルシウム動員の測定法-fura-2を中心として-2004

    • Author(s)
      飯田秀利
    • Total Pages
      9
    • Publisher
      廣川書店
    • Related Report
      2004 Annual Research Report
  • [Book] 細胞内pH測定法2004

    • Author(s)
      飯田秀利
    • Total Pages
      6
    • Publisher
      廣川書店
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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