| Project/Area Number |
16370075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Hyogo |
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Principal Investigator |
TSUDA Motoyuki University of Hyogo, Department of Life Science, Professor, 大学院生命理学研究科, 教授 (60045458)
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| Co-Investigator(Kenkyū-buntansha) |
KUSAKABE Takehiro University of Hyogo, Department of Life Science, Asociate Professor, 大学院生命理学研究科, 助教授 (40280862)
NAKAGAWA Masashi University of Hyogo, Department of Life Science, Research Asistant, 大学院生命理学研究科, 助手 (00212085)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2005: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2004: ¥6,100,000 (Direct Cost: ¥6,100,000)
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| Keywords | Phtoreceptor / Rhodopsin / Swimming behavior / Motor neuron / Ascidian / WGA / GFP / Nervous system |
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| Research Abstract |
We showed that the ascidian larvae show photic swimming 4 h after hatching, the latter half of larval. The larvae start swimming when light intensity decreases and stop swimming when the intensity of light is increased. Since the ascidian larva, has a most simple nervous system with only about 100 neurons, it is expected that neural pathway from photoreceptor to muscle will be solved The selective and functional neural transsynaptic pathways form the photoreceptors to the uscle of Ciona Intesnalis larva were successfully visualized by a novel WGA trnasgene technique under the control of the photoreceptor specific gene (arrestin) and the motor neuron specific gene (vesicle acetylcholine transporter promoter).
WGA expressed in the photoreceptor cells was transferred to the second order-order neurons in the brain vesicle. Further WGA was detected in the forth pair of third pair of acetylcholine positive cell in visceral ganglion. WGA expressed in the acetylcholine positive cells visualized somat as well as axon and nerve terminal of five pairs of the acetylcholine positive cells Nerve terminals of the first, the second, and the fifth pair of the acetylcholine positive cells form neuron-muscle junction which may innovate muscle. Axon of the third acetylcholine positive cell run to retrograde direction and may not be motor neuron. The forth pair of the acetylcholine positive cells extend their axon to anterograde direction to neurve cord and may control the other motor neurons and the interneurons..
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