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Analysis of molecular mechanism of homologous recombination in eukaryotes

Research Project

Project/Area Number 16370080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

SHINOHARA Akira  Osaka University, Institute for Protein Research, Professor, 蛋白質研究所, 教授 (00252578)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2005: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2004: ¥9,700,000 (Direct Cost: ¥9,700,000)
Keywordsrecombination / DNA repair / meiosis / gonome instability / 細胞周期 / 酵母
Research Abstract

Homologous recombination, an exchange between DNA strands, plays a role in the maintenance of genome stability and the production of genome diversity. While, in mitosis, it is required for the repair of DNA damage, it is for the segregation of homologous chromosome at meiotic division I. Malfunction of the recombination leads cancer and infertility in human. In order to reveal molecular mechanism of the recombination, we constructed a strain in which a single doubles-strand break site are visualized with Green Fluorescent protein. Using the strain, the assembly/ disassembly of proteins are monitored. Our results suggest that proteins involved in recombination form a ternary complex on ssDNA, whose assembly/disassembly is tightly coupled with the biochemical transition of recombination intermediates. In addition, we have been looking for new genes involved in meiotic recombination in order to get insight in the mechanism of the recombination between homologs. We found that two proteins, Mei5 and Sae3, form a complex, which helps assembly of a meiosis-specific RecA homolog, Dmc1 and cooperates with Dmc1 for the recombination. Furthermore, we also found three new genes involved in meiotic recombination (Spo16, Csm4, Ydr015C) and have been analyzing the phenotypes of the mutants to know the function of the proteins in meiotic recombination.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (20 results)

All 2005 2004

All Journal Article (20 results)

  • [Journal Article] 減数分裂期において相同染色体間の組換えを促進する新規複合体2005

    • Author(s)
      篠原 彰 他
    • Journal Title

      実験医学 23

      Pages: 1562-1565

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of novel Xrs2 mutation In Saccharomyces cerevisiae2005

    • Author(s)
      Shima K
    • Journal Title

      Genetics 170

      Pages: 71-85

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 減数分裂期において相同染色体間の組換えを促進する新規複合体2005

    • Author(s)
      篠原 彰
    • Journal Title

      実験医学 23

      Pages: 1562-1565

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 減数分裂期のDNAの交換反応2005

    • Author(s)
      篠原 彰
    • Journal Title

      生化学 77

      Pages: 415-419

    • NAID

      10015653519

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 減数分裂期の相同組換えに働く新規蛋白質複合体の機能2005

    • Author(s)
      篠原 彰
    • Journal Title

      生物物理 (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific homolog RecA Dmc1.2004

    • Author(s)
      Hayase, A. 他
    • Journal Title

      Cell 119

      Pages: 927-940

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.2004

    • Author(s)
      Miyazaki T. 他
    • Journal Title

      EMBO J. 23

      Pages: 939-949

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis.2004

    • Author(s)
      Yamashita, K. 他
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 11380-11385

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mnd1 is required for meiotic inter-homolog repair.2004

    • Author(s)
      Zierhut, C. 他
    • Journal Title

      Current Biology 14

      Pages: 752-762

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Roles of RecA homologues Rad51 and Dmc1 during meiotic recombination.2004

    • Author(s)
      Shinohara, A. 他
    • Journal Title

      Cytogenetics and Genome Research 107

      Pages: 201-207

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific homolog RecA Dmc1.2004

    • Author(s)
      Hayase, et al.
    • Journal Title

      Cell 119

      Pages: 927-940

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.2004

    • Author(s)
      Miyasaki T., et al.
    • Journal Title

      EMBO J. 23

      Pages: 939-949

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis.2004

    • Author(s)
      Yamashita K., et al.
    • Journal Title

      Proc.Natl.Acad, Sci.USA 101

      Pages: 11380-11385

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mnd1 is required for meiotic inter-homolog repair.2004

    • Author(s)
      Zierhut C., et al.
    • Journal Title

      Current Biology 14

      Pages: 752-762

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Roles of RecA homologues Rad51 and Dmc1 during meiotic recombination.2004

    • Author(s)
      Shinohara, A., Shinohara, M.
    • Journal Title

      Cytogenetics & Genome Research 107

      Pages: 201-207

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific homolog RecA Dmc1.2004

    • Author(s)
      Hayase, A.他
    • Journal Title

      Cell 119

      Pages: 927-940

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.2004

    • Author(s)
      Miyazaki T.他
    • Journal Title

      EMBO J. 23

      Pages: 939-949

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis.2004

    • Author(s)
      Yamashita, K.他
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 11380-11385

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mnd1 is required for meiotic inter-homolog repair.2004

    • Author(s)
      Zierhut, C.他
    • Journal Title

      Current Biology 14

      Pages: 752-762

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Roles of RecA homologues Rad51 and Dmc1 during meiotic recombination.2004

    • Author(s)
      Shinohara, A.他
    • Journal Title

      Cytogenetics and Genome Research 107

      Pages: 201-207

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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