• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cross-talk between cell-external cues and cell-intrinsic programs involved in cell fate determination

Research Project

Project/Area Number 16370088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKumamoto University

Principal Investigator

TAGA Tetsuya  Kumamoto University, Institute of Molecular Embryology and Genetics, Professor, 発生医学研究センター, 教授 (40192629)

Co-Investigator(Kenkyū-buntansha) KAGAWA Tetsushi  Kumamoto University, Institute of Molecular Embryology and Genetics, Associate Professor, 発生医学研究センター, 助教授 (50270484)
NOBUHISA Ikuo  Kumamoto University, Institute of Molecular Embryology and Genetics, Assistant Professor, 発生医学研究センター, 講師 (40332879)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2005: ¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 2004: ¥8,500,000 (Direct Cost: ¥8,500,000)
Keywordsneural stem cell / hematopoietic stem cell / signal transduction / cell proliferation / cell differentiation / Wnt / AGM region / cytokine / HIPK2 / 転写因子 / ニューロン / アストロサイト / オリゴデンドロサイト
Research Abstract

(1)We have previously shown that BMPs inhibit neurogenesis while inducing astrogliogenesis. In this study, functional recovery of recipient mice with spinal cord injury was observed when neural stem cells expressing noggin, a BMP inhibitor, were transplanted.
(2)Olig2 is an essential transcription factor for oligodendrocyte lineage specification and is expressed predominantly in ventral neuroepithelial cells in the medial and lateral ganglionic eminence (GE), where oligodendrocyte progenitors originate. In this study, we show significant induction of Olig2 expression in dorsal neuroepithelium-derived cells cultured in the presence of bFGF. We also show that bFGF endows these cells with the ability to differentiate into oligodendrocytes.
(3)In this study, we show that many cancer cell lines contain a small side population (SP), which, in many normal tissues, is thought to contain stem cells of the tissue. We propose that cancer cell lines contain a minor subpopulation of stem cells that is enriched in an SP, can be maintained indefinitely in culture, and is crucial for their malignancy.
(4)We here show that bFGF and Wnt3a cooperate together to induce cyclin D1 in neural stem cells, which leads to enhanced cell cycle progression and attenuation of astrogliogenesis of these cells.
(5)The aorta-gonad-mesonephros (AGM) region is a primary source of definitive hamotopoietic cells in the midgestation mouse embryo. In this study, we performed characterization of hematopoietic cells that emerge in the AGM culture. Based on the expression profile of CD45 and c-Kit examined by flow cytometry, we defined three cell populations : CD45^<low> c-Kit^+ (population 1), CD45^<low> c-Kit^- (population 2), and CD45^<high> c-Kit^<low/-> (population 3) cells. From in vitro colony-forming assays and in vivo transplantation experiments, we conclude that the CD45^<low> c-Kit^+ cells produced in the AGM culture have the potential to reconstitute multi-lineage hematopoietic cells.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (43 results)

All 2006 2005 2004 Other

All Journal Article (41 results) Book (2 results)

  • [Journal Article] bFGF endows dorsal teelemcephalic neural progenitors with ability to differentiate into oligodendrocytes but not GABAergic neurons.2006

    • Author(s)
      M.Abematsu, et al.
    • Journal Title

      J. Neurosci. Res. 83

      Pages: 731-743

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enhanced engraftment of hematopoietic stem/progenitor cells by the transient inhibition of an adaptor protein, Lnk.2006

    • Author(s)
      H.Takizawa, et al.
    • Journal Title

      Blood 107

      Pages: 2968-2975

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activation of canonical Wnt pathway promotes proliferation of retinal stem cells derived from adult mouse ciliary margin.2006

    • Author(s)
      T.Inoue, et al.
    • Journal Title

      Stem Cells 24

      Pages: 95-124

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] bFGF endows dorsal teelemcephalic neural progenitors with ability to differentiate into oligodendrocytes but not GABAergic neurons.2006

    • Author(s)
      M.Abematsu, et al.
    • Journal Title

      J.Neurosci.Res. 83

      Pages: 731-743

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fate redirection of hippocampal astrocytes toward neuronal lineage by aggregate culture.2005

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      Neurosci. Res. 53

      Pages: 176-182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells.2005

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      J. Neurochem. 95

      Pages: 1311-1320

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preferential differentiation of neural progenitor cells into the glial lineage through gp130 signaling in N-methyl-D-aspartate-treated retinas.2005

    • Author(s)
      Y.Mawatari, et al.
    • Journal Title

      Brain Res. 1055

      Pages: 7-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Essential role of STAT3 in cytokine-driven NF-kappaB-mediated serum amyloid A gene expression.2005

    • Author(s)
      K.Hagihara, et al.
    • Journal Title

      Genes Cells 11

      Pages: 1051-1063

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Glycosphingolipid synthesis inhibitor represses cytokine-induced activation of the Ras-MAPK pathway in embryonic neural precursor cells.2005

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      J. Biochem. 138

      Pages: 285-291

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of Interleukin-6 in the neural stem cell differentiation.2005

    • Author(s)
      T.Taga, et al.
    • Journal Title

      Clini. Rev. Allergy Immnol. 28

      Pages: 249-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stage-and site-specific SNA demethylation during neural cell development from embryonic stem cells.2005

    • Author(s)
      K.Shimozaki, et al.
    • Journal Title

      J. Neurochem. 93

      Pages: 432-439

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cell fate determination regulated by a transcriptional signalnetwork in the developing mouse brain.2005

    • Author(s)
      S.Fukuda, et al.
    • Journal Title

      Anat. Sci. Int. 80

      Pages: 12-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fate redirection of hippocampal astrocytes toward neuronal lineage by aggregate culture.2005

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      Neurosci.Res. 53

      Pages: 176-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells.2005

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      J.Neurochem. 95

      Pages: 1311-1320

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Preferential differentiation of neural progenitor cells into the glial lineage through gpl30 signaling in N-methyl-D-aspartate-treated retinas.2005

    • Author(s)
      Y.Mawatari, et al.
    • Journal Title

      Brain Res. 1055

      Pages: 7-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glycosphingolipid synthesis inhibitor represses cytokine-induced activation of the Ras-MAPK pathway in embryonic neural precursor cells.2005

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      J.Biochem. 138

      Pages: 285-291

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of Interleukin-6 in the neural stem cell differentiation.2005

    • Author(s)
      T.Taga, et al.
    • Journal Title

      Clini.Rev.Allergy Immnol. 28

      Pages: 249-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Stage-and site-specific SNA demethylation during neural cell development from embryonic stem cells.2005

    • Author(s)
      K.Shimozaki, et al.
    • Journal Title

      J.Neurochem. 93

      Pages: 432-439

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Cell fate determination regulated by a transcriptional sign alnetwork in the developing mouse brain.2005

    • Author(s)
      S.Fukuda, et al.
    • Journal Title

      Anat.Sci.Int. 80

      Pages: 12-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glycosphingolipid synthesis inhibitor represses cytokine-induced activation of the Ras-MAPK pathway in embryonic neural precursor cells.2005

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      J.Biochemistry 138

      Pages: 285-291

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stage-and site-specific DNA demethylation during neural cell development from embryonic stem cells.2005

    • Author(s)
      K.Shimozaki, et al.
    • Journal Title

      J.Neurochem. 93

      Pages: 432-439

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cell fate determination regulated by a transcriptional signal network in the developing mouse brain.2005

    • Author(s)
      S.Fukuda, et al.
    • Journal Title

      Anat.Sci.Int. 80

      Pages: 12-18

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Targeted disruption of the mouse ELYS gene results in embryonic death at peri-implantation development.2004

    • Author(s)
      K.Okita, et al.
    • Journal Title

      Genes Cells 9

      Pages: 1083-1091

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Roles of lipid rafts in integrin-dependent adhesion and gp130 signaling pathway in mouse embryonic neural precursor cells.2004

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      Genes Cells 9

      Pages: 801-809

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development stage dependent regulation of DNA methylation and modification in a immature astrocyte specific gene promoter.2004

    • Author(s)
      M.Namihira, et al.
    • Journal Title

      FEBS Lett. 572

      Pages: 184-188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Treatment of spinal cord injury by transplantation of fetal neural precursor cells engineered to express BMP inhibitor.2004

    • Author(s)
      T.Setoguchi, et al.
    • Journal Title

      Exp. Neurol. 189

      Pages: 33-44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cancer stem cells persist in many cancer cell lines.2004

    • Author(s)
      T.Setoguchi, et al.
    • Journal Title

      Cell Cycle 3

      Pages: 414-415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line.2004

    • Author(s)
      T.Kondo, et al.
    • Journal Title

      Proc. Natl. Acid. Sci. USA 101

      Pages: 781-786

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Spred-2 suppresses aorta-gonad mesonephros hematopoiesis by inhibiting MAP kinase activation.2004

    • Author(s)
      I.Nobuhisa, et al.
    • Journal Title

      J. Exp. Med. 199

      Pages: 737-742

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Negtive regulatory effect of an oligodendrocytic bHLH factor OLIG2 on the astrocytic differentiation pathway.2004

    • Author(s)
      S.Fukuda, et al.
    • Journal Title

      Cell Death Differ. 11

      Pages: 196-202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Negative regulation of hematopoiesis by the fused in myeloproliferative disoeders gene product.2004

    • Author(s)
      H.Inoue, et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 313

      Pages: 125-128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Targeted disruption of the mouse ELYS gene results in embryonic death at pert-implantation development.2004

    • Author(s)
      K.Okita, et al.
    • Journal Title

      Genes Cells 9

      Pages: 1083-1091

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Treatment of spinal cord injury by transplantation of fetal neural precursor cells engineered to express BMP inhibitor.2004

    • Author(s)
      T.Setoguchi, et al.
    • Journal Title

      Exp.Neurol. 189

      Pages: 33-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line.2004

    • Author(s)
      T.Kondo, et al.
    • Journal Title

      Proc.Natl.Acid.Sci.USA 101

      Pages: 781-786

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Spred-2 suppresses aorta-gonad mesonephros hematopoiesis by inhibiting MAP kinase activation.2004

    • Author(s)
      I.Nobuhisa, et al.
    • Journal Title

      J.Exp.Med. 199

      Pages: 737-742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Negative regulation of hematopoiesis by the fused in myeloproliferative disoeders gene product.2004

    • Author(s)
      H, Inoue, et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 313

      Pages: 125-128

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Targeted disruption of the mouse ELYS gene results in embryonic death at peri-implantation development.2004

    • Author(s)
      K.Okita, et al.
    • Journal Title

      Genes to Cells 9

      Pages: 1093-1091

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Roles of lipid rafts in integrin-dependent adhesion and gp130 signaling pathway in mouse embryonic neural precursor cells.2004

    • Author(s)
      M.Yanagisawa, et al.
    • Journal Title

      Genes to Cells 9

      Pages: 801-809

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Negative regulation of hematopoiesis by the fused in myeloproliferative disoeders gene product.2004

    • Author(s)
      H.Inoue, et al.
    • Journal Title

      Biochem.Bophys.Res.Commun. 313

      Pages: 125-128

    • Related Report
      2004 Annual Research Report
  • [Journal Article] bFGF endows dorsal teelemcephalic neural progenitors with ability to differentiate into oligodendrocytes but not GABAergic neurons.

    • Author(s)
      M.Abematsu, et al.
    • Journal Title

      J.Neurosci.Res. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Enhanced engraftment of hematopoietic stem/progenitor cells by the transient inhibition of an adaptor protein, Lnk.

    • Author(s)
      H.Takizawa, et al.
    • Journal Title

      Blood (in press)

    • Related Report
      2005 Annual Research Report
  • [Book] ここまで進んだ幹細胞研究と再生医療2006

    • Author(s)
      田賀哲也, 中畑龍俊 共編
    • Total Pages
      208
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] ここまで進んだ幹細胞研究と再生医療20062006

    • Author(s)
      田賀哲也, 中畑龍俊 共編
    • Total Pages
      208
    • Publisher
      羊土社
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi