• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of genetic factors controlling useful traits for Sake-brewing and development of evaluation methods on genetic resources of Sake-brewing rice

Research Project

Project/Area Number 16380006
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKobe University

Principal Investigator

KAMIJIMA Osamu  Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (30031222)

Co-Investigator(Kenkyū-buntansha) ISHII Takashige  KOBE University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20260648)
MORI Naoki  Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60230075)
NAKAMURA Chiharu  Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (10144601)
吉田 晋也  兵庫県立農林水産技術総合センター, 部長(生物工学担当)付, 主任研究員
池上 勝  兵庫県立農林水産技術総合センター, 作物部・酒米試験地, 主任研究員
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2006: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥9,100,000 (Direct Cost: ¥9,100,000)
KeywordsSake-brewing rice / Useful traits for Sake-brewing / QTL analysis / Prolamin / SSR marker / White core / 胚乳 / 連鎖不平衡 / SAGE / DNAマーカー / フィンガープリンティング
Research Abstract

1. Genetic analysis on white core and grain size was carried out using the following populations of rice.
-F1 populations generated from diallel crosses between five cultivars for Sake-brewing and cooking rice.
-Fl, F2 and backcross populations between Gohyakumangoku and Koshihikari.
In addition, QTL analysis on white core and grain size was performed using recombinant inbred lines between Hyogokitanishiki and Nipponbare. As a result, major QTLs for these traits were detected on chromosomes 2 and 12.
2. In order to identify the genes controlling useful traits for Sake-brewing, gene expression survey was carried out by SAGE method with ripening seeds of Yamadanishiki (Sake-brewing rice) and Reiho (cooking rice). One of the candidate genes showing strong variety-specific expression was Rsg1 which codes 13 kDa of prolamin. Based on the gene structural analysis, Reiho was found to keep intact Rsg1 gene, whereas Yamadanishiki has inactive one because of the insertion of transposon-like sequences. QTL analysis on seed protein content was also carried out using 91 double haploid lines between Yamadanishiki and Reiho, and one QTL for protein content of 13 kDa prolamin was detected on chromosome 12, suggesting this locus correspond to Rsg1 gene.
3. Allelic diversity at 76 SSR loci among 188 cultivars of Sake-brewing and cooking rice was examined. Based on these data, principal component analysis, cluster analysis and group structural analysis (by Markov Chain Monte Carlo Method) were carried out. As a result, 188 cultivars were classified into 10 subgroups. Of these, three groups (named as Yamadanishiki, Gohyakumangoku and Hanafubuki groups) were found to consist of majority of Sake-brewing rice cultivars.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (16 results)

All 2007 2006 2005 2004

All Journal Article (16 results)

  • [Journal Article] 「山田錦」の持つ穂数に関する遺伝子の育種的考察.2007

    • Author(s)
      吉田晋弥
    • Journal Title

      兵庫県立農林水産技術総合センター研究報告(農業) 55

      Pages: 10-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The view on breeding for 'Yamada-Nishiki' allele on the quantitative trait loci for panicle number2007

    • Author(s)
      Shinya, Yoshida
    • Journal Title

      Bulletin of The Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries (Agriculture Sections) 55

      Pages: 10-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic analysis of super long chain synthesis in rice amylopectin2006

    • Author(s)
      Noriaki, Aoki
    • Journal Title

      Euphytica 第151巻・第2号

      Pages: 225-234

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Genetic analysis of super long chain synthesis in rice amylopectin2006

    • Author(s)
      Noriaki, Aoki
    • Journal Title

      Euphytica 151-2

      Pages: 225-234

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 酒米のルーツと酒造好適性遺伝子を探る2006

    • Author(s)
      森 直樹
    • Journal Title

      第30回酒米懇談会要旨集

      Pages: 23-30

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 数種の酒米品種間の交雑F_1個体における心白発現と粒大の遺伝解析2006

    • Author(s)
      池上 勝
    • Journal Title

      育種学研究 第8巻別冊1号(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 酒米を中心とした水稲遺伝資源のDNA多型.2005

    • Author(s)
      吉田晋弥
    • Journal Title

      兵庫県立農林水産技術総合センター研究報告(農業) 53

      Pages: 28-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 酒米品種「山田錦」の育成経過と母本品種「山田穂」,「短稈渡船」の来歴.2005

    • Author(s)
      池上 勝
    • Journal Title

      兵庫県立農林水産技術総合センター研究報告(農業) 53

      Pages: 37-50

    • NAID

      40006703129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] DNA polymorphism and genetic diversity on the brewing rice genetic resources2005

    • Author(s)
      Shinya, Yoshida
    • Journal Title

      Bulletin of The Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries (Agriculture Sections) 53

      Pages: 28-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Breeding process of Sake-brewing rice variety 'Yamadanishiki' and the history of parental varieties 'Yamadabo' and 'Tankan-Wataribune'2005

    • Author(s)
      Masaru, Ikegami
    • Journal Title

      Bulletin of The Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries (Agriculture Sections) 53

      Pages: 37-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 五百万石とコシヒカリの交配後代における心白発現と粒大の遺伝解析2005

    • Author(s)
      池上 勝
    • Journal Title

      育種学研究 第7巻別冊1・2号

      Pages: 416-416

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic diversity and phylogeny of Japanese sake-brewing rice as revealed by molecular markers.2005

    • Author(s)
      Mori, N.
    • Journal Title

      5^<th> International Rice Genetics Symposium

      Pages: 127-127

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Profiling of endosperm gene expression in Sake-brewing rice by SAGE2005

    • Author(s)
      Takahashi, T.
    • Journal Title

      5^<th> International Rice Genetics Symposium

      Pages: 177-177

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic diversity and phylogeny of Japanese sake-brewing rice as revealed by AFLP and nuclear and chloroplast SSR markers.2004

    • Author(s)
      Hashimoto, Z.
    • Journal Title

      Theoretical and Applied Genetics 第109巻第8号

      Pages: 1586-1596

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Genetic diversity and phylogeny of Japanese sake-brewing rice as revealed by AFLP and nuclear and chloroplast SSR markers2004

    • Author(s)
      Zentaro, Hashimoto
    • Journal Title

      Theoretical and Applied Genetics 109-8

      Pages: 1586-1596

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] イネ品種「兵庫北錦」と「北陸142号」の雑種集団を用いた心白発現および粒大に関するQTL解析2004

    • Author(s)
      澤田桂子
    • Journal Title

      育種学研究 第6卷別冊2号

      Pages: 202-202

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi