Study for genetic diversity of 'uzu'semi-dwarfbarley varieties in evolutionary, morphological and physiological aspects.

• Project/Area Number: 16380008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Okayama University
• Principal Investigator: TAKEDA Kazuyoshi Okayama University, Research Institute for Bioresources, Professor (90003516)
• Co-Investigator(Kenkyū-buntansha): SAISHO Daisuke OKAYAMA UNIVERSITY, Research Institute for Bioresources, Assistant Professor (90325126) ; RIKIISHI Kazuhide OKAYAMA UNIVERSITY, Research Institute for Bioresources, Assistant Professor (90220798)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥15,600,000); Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2005: ¥4,400,000 (Direct Cost: ¥4,400,000); Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
• Keywords: barley / uzu / diversity

Research Abstract

In order to dissect the diversity of semi-dwarf barley variety 'uzu' in evolutionary, morphological and physiological aspects , we analyzed the genetic diversity of barley uzu varieties from three view points:(1) phylogeny of 'uzu' accessions,(2) genetic dissection of the 'uzu' accession specific morphological trait including the emergence of projection on coleoptile under high temperature condition, and (3) the physiological diversity of tissue culture traits including callus growth and shoot regeneration capacity. Results from these studies are:
(1) Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in 282 barley accessions including 19 wild barley lines reveal that landraces distributed in South and East Asia are genetically distinct from those in 'Occidental' region. The six 'uzu' accessions investigated in this study possessed almost same haplotype among the five loci. These evidence strongly indicated that the origin of 'uzu' accessions was si ngle mutation even.
(2) One of the 'uzu'-specific morphological traits, the emergence of projection on coleoptile under high temperature germination condition, is caused by ectopic cell division of coleoptiles apart from embryo genesis. Evaluation of this trait among 266 'uzu' accessions and segregation analysis of five F2 populations indicate that genetic factor (s) concerning to this character are genetically diverse within 'uzu' accessions. As the result of diallel analysis using 10 x 10 half diallel table, it is suggested that this trait is controlled by several additive genes.
(3) Effects of exogenous auxin and cytokinin on the emergence of projection on coleoptile were examined in three independent isogenic lines of barley with uzu gene. Although the emergence of projection was reduced in uzu lines under low temperature condition, both auxin and cytokinin enhanced the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection on coleoptile

Research Products

• [Journal Article] Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway. (2008)
  • Author(s): Taketa, S., S. Amano, Y. Tsujino, T. Sato, D. Saisho, K. Kakeda, M. Nomura, T. Suzuki, T. Matsumoto, K. Sato, H. Kanamori, S. Kawasaki and K. Takeda.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 4062-4067
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway (2008)
  • Author(s): Taketa, S., S., Amano, Y., Tsujino, T., Sato, D., Saisho, K., Kakeda, M., Nomura, T., Suzuki, T., Matsumoto, K., Sato, H., Kanamori, S., Kawasaki, K., Takeda
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 4062-4067
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library from Japanese Malting Barley 'Haruna Nijo'. (2007)
  • Author(s): Saisho, D., E. Myoraku, S. Kawasaki, K. Sato and K. Takeda.
  • Journal Title: Breed. Sci. 54 Pages: 409-416
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular phylogeography of domesticated barley traces expansion of agriculture in the Old World. (2007)
  • Author(s): Saisho D. and Michael D. Purugganan
  • Journal Title: GENETICS 177 Pages: 1765-1776
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library from Japanese Malting Barley Haruna Nijo (2007)
  • Author(s): Saisho, D., E., Myoraku, S., Kawasaki, K., Sato, K., Takeda
  • Journal Title: Breed. Sci 54 Pages: 409-416
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Purugganan. Molecular phylogeography of domesticated barley traces expansion of agriculture in the Old World (2007)
  • Author(s): Saisho, D., Michael, D
  • Journal Title: GENETICS 177 Pages: 1765-1776
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Linkage map construction and QTL detection based on barley ESTs in Agenome diploid wheat. (2007)
  • Author(s): Hori K.
  • Journal Title: Breed. Sci. 57 Pages: 39-45
• [Journal Article] Construction and characterization of a bacterial artificial chromosome (BAC) library for Japanese malting barley 'Haruna Ni jo'. (2007)
  • Author(s): Saisho D.
  • Journal Title: Breed. Sci. 57 Pages: 29-38
• [Journal Article] QTL analysis of Fusarium head blight resistance severity in recombinant inbread population derived from a cross between two-rowed barley varieties. (2006)
  • Author(s): Hori K.
  • Journal Title: Breed. Sci. 56 Pages: 25-30
• [Journal Article] Genomic distribution of MITEs in barley determined by MITE-AFLP mapping. (2006)
  • Author(s): Takahashi H.
  • Journal Title: Genome 49 Pages: 1616-1620
• [Journal Article] Mapping a factor controlling the thermostability of seed lipoxygenase-1 in barley. (2006)
  • Author(s): Hirota N.
  • Journal Title: Plant Breed. 125 Pages: 231-235
• [Journal Article] Geographical differentiation and diallel analysis of seed dormancy in barley. (2006)
  • Author(s): Takeda K.
  • Journal Title: Euphytica
• [Journal Article] QTL analysis of Fusarium Head Blight severity in recombinant inbred population derived from a cross between two-rowed barley varieties. (2006)
  • Author(s): K.Hori et al.
  • Journal Title: Breed.Sci. 56 Pages: 25-30
• [Journal Article] QTL analysis in recombinant chromosome substitution lines and doubled haploid lines derived from a cross between Hordeum vulgare ssp. vulgare and Hordeum vulgare ssp. spontaneum. (2005)
  • Author(s): K.Hori et al.
  • Journal Title: Molecular Breed. 16 Pages: 295-311
• [Journal Article] QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley. (2005)
  • Author(s): K.Hori et al.
  • Journal Title: Theor.Appl.Genet. 111 Pages: 1661-1672
• [Journal Article] Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting two-pair primers. (2004)
  • Author(s): Domon, E., Y.Yanagisawa, A.Saito, K.Takeda
  • Journal Title: Plant Breed. 123 Pages: 225-228
• [Journal Article] β-amylase variation in wild barley accessions. (2004)
  • Author(s): Zhang, W., T.Kaneko, K.Takeda
  • Journal Title: Breed.Sci. 5 Pages: 41-49
  • NAID: 110001803264
• [Journal Article] Differentiation of β-amylase phenotypes in cultivated barley. (2004)
  • Author(s): Zhang, W., T.Kaneko, M.Ishii, K.Takeda
  • Journal Title: Crop Sci. 44 Pages: 1608-1614
• [Journal Article] Molecular mapping of a gene responsible for Al-activated secretion of citrate in barley. (2004)
  • Author(s): Ma, J.F., S.Nagao, K.Sato, H.Ito, J.Furukawa, K.Takeda
  • Journal Title: J.Exp.Bot. 55 Pages: 1335-1341
• [Journal Article] Spontaneous Brassinolide-Insensitive Barley Mutants ‘uzd' Adapted to East Asia. (2004)
  • Author(s): D.Saisho, K.Tanno, M.Chono, I.Honda.H.Kitano, K.Takeda
  • Journal Title: Breed.Sci. 54 Pages: 409-416
• [Journal Article] Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos.
  • Author(s): Rikiishi, K., D. Saisho and K. Takeda.
  • Journal Title: Breed. Sci. (in press)
  • NAID: 110006781641
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos
  • Author(s): Rikiishi, K., D., Saisho, K., Takeda
  • Journal Title: Breed. Sci (in press)
  • NAID: 110006781641
  • Description: 「研究成果報告書概要(欧文)」より