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Resolution of accumulation mechanism into protein body of prolamin in rice seed

Research Project

Project/Area Number 16380009
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKyushu University

Principal Investigator

KUMAMARU Toshihiro  Kyushu University, Faculty of Agriculture, Associate professor (00284555)

Co-Investigator(Kenkyū-buntansha) SATOH Hikaru  Kyushu University, Faculty of Agriculture, Professor (70128031)
OGAWA Masahiro  Yamaguchi Prefectural University, Faculty of Life Science, Professor (10160772)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,660,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2007: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsCell. Tissue / Genetics / Genome / Seed / Rice / Storage Protein / 遺伝学 / ゲノム / 植物 / PB-I / 翻訳終結因子 / 相補性検定 / RT-PCR / 小胞体 / 突然変異 / プロラミン / 胚乳 / 集積 / 翻訳
Research Abstract

Accumulation of prolamin into protein body
In rice cystein rich prolamin, which is composed of three polypeptides, 10 kDa, 15 kDa, and 16 kDa, forms the prolamin protein body with 13 kDa cystein poor prolamin. Immunofluorescent and immunoelectron microscopies indicated that 10 and 13 kDa prolamins localized to central and peripheral regions of the PB. Additionally, immunofluorescent microscopy showed that 10 kDa prolamin was accumulated into ER lumen at 7 day after flowering and after that 13 kDa prolamin was layered over the core of 10 kDa prolamin. In the 13 kDa cystein poor prolamin deficient mutant, esp1, spherical PB with the electron dense core including 10kDa prolamin was observed. In the 10 kDa prolamin deficient mutant, esp3, the core structure of PB was not observed, instead, irregular, electron lucent and hypertrophied PB was observed. These results suggest that the formation of prolamin PB in rice is initiated by accumulation of 10 kDa prolamin in the central core and sequentially 13 kDa prolamin is layered over the core structure. It is concluded that 10 kDa prolamin plays a role to form the core structure to stabilize prolamin PB structure in rice.
Identification and function of Esp1 gene
In esp1 mutation, cystein poor prolamin is reduced. In order to specify the responsible gene of esp1 mutation, the construction of the high-density linkage map of esp1 gene and the complementation test were performed. As results, it became clear that Esp1 gene encode the Eucaryotic Release Factor 1(eRFF1), which is translation termination factor, indicating that the eRF1 is a factor regulating the translation of cystein poor prolamin.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (43 results)

All 2008 2007 2006 2005 2004 Other

All Journal Article (26 results) (of which Peer Reviewed: 8 results) Presentation (15 results) Book (2 results)

  • [Journal Article] MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice2008

    • Author(s)
      Suzuki T., et. al.
    • Journal Title

      Mol.Gen.Gen. 279

      Pages: 213-223

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice2008

    • Author(s)
      Suzuki, T
    • Journal Title

      Mol. Gen. Gen 279

      Pages: 213-223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice2008

    • Author(s)
      Suzuki T., et al.
    • Journal Title

      Mol.Genet.Genomics 279

      Pages: 213-223

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein body biogenesis2007

    • Author(s)
      Kumamaru, T., et. al.
    • Journal Title

      cereal endosperms.In Endosperm-Development and Molecular Biology, O.A.Olsen, ed.Plant Cell Monographs(8), Springer

      Pages: 141-158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protein body biogenesis in cereal endosperms. In Endosperm - Development and Molecular Biology, O. Olsen, ed2007

    • Author(s)
      Kumamaru, T
    • Journal Title

      Plant Cell Monographs(8) Springer

      Pages: 141-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Rice protein digestion by sake Koji enzyme:Comparison between steamed rice grains and isolated protein bodies from rice endosperm2006

    • Author(s)
      Hashizume, K., et. al.
    • Journal Title

      J.Biosci.and Bioeng 102

      Pages: 340-345

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Rice protein digestion by sake Koji enzyme : Comparison between steamed rice grains and isolated protein bodies from rice endosperm2006

    • Author(s)
      Hashizume, K
    • Journal Title

      J. Biosci. and Bioeng 102

      Pages: 340-345

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The role of mRNA and protein sorting in seed storage protein synthesis,transport,and deposition2005

    • Author(s)
      Crofts A J., et. al.
    • Journal Title

      Biochem.Cell Biol. 83

      Pages: 728-737

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A modified TILLING system for rice mutant screening2005

    • Author(s)
      Suzuki T., et. al.
    • Journal Title

      Rice Genetics Newsletter 22

      Pages: 89-90

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The role of mRNA and protein sorting in seed storage protein synthesis, transport, and deposition2005

    • Author(s)
      Crofts, A. J
    • Journal Title

      Biochem. Cell Biol 83

      Pages: 728-737

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A modified TILLING system for rice mutant screening2005

    • Author(s)
      Suzuki, T
    • Journal Title

      Rice Genetics Newsletter 22

      Pages: 89-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The role of mRNA and protein sorting in seed storage protein synthesis, transport, and deposition2005

    • Author(s)
      Crofts A J., H.Washida, T.W.Okita, M.Satoh, M.Ogawa, T.Kumamaru, H.Satoh
    • Journal Title

      Biochem.Cell Biol. 83

      Pages: 728-737

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources2005

    • Author(s)
      Jahan Md.S., Y.Uemura, T.Kumamaru, A.Hamid, H.Satoh
    • Journal Title

      Genetic Resources and Crop Evolution 52

      Pages: 977-987

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources2005

    • Author(s)
      Jahan Md.S., Y.Uemura, T.Kumamaru, A.Hamid, H.Satoh
    • Journal Title

      Genetic Resources and Crop Evolution (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Targeting of proteinsto ER-derived compartments in plants:The importance of RNA localization2004

    • Author(s)
      Crofts A.J., et. al.
    • Journal Title

      Plant Physiology 136

      Pages: 3414-3419

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 心白を持つ胚乳変異体米の構造観察と酒造適性分析2004

    • Author(s)
      荒巻 功, ら
    • Journal Title

      日本醸造協会誌 99

      Pages: 134-145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 心白を持つ胚乳変異体米の粘度及び糊化特性と清酒醸造試験2004

    • Author(s)
      荒巻 功, ら
    • Journal Title

      日本醸造協会誌 99

      Pages: 189-201

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Targeting of proteins to ER-derived compartments in plants : The importance of RNA localization2004

    • Author(s)
      Crofts, A.J
    • Journal Title

      Plant Physiology 136

      Pages: 3414-3419

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Observation of the endosperm mutant rice grains having a white core and analysis of their physical and chemical properties for Sake brewing2004

    • Author(s)
      Aramaki, I
    • Journal Title

      J. Brew. Soc. Japan 99

      Pages: 134-135

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pasting and gelatinization properties of endosperm mutants rice grains having a white core and analysis of their suitability for Sake brewing2004

    • Author(s)
      Aramaki, I
    • Journal Title

      J. Brew. Soc. Japan 99

      Pages: 189-201

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Targeting of proteins to ER-derived compartments in plants : The importance of RNA localization2004

    • Author(s)
      A.J.Crofts, H.Washida, T.W.Okita, T.Kumamaru, H.Satoh
    • Journal Title

      Plant Physiology 136

      Pages: 3414-3419

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 心白を持つ胚乳変異体米の構造観察と酒造適性分析2004

    • Author(s)
      荒巻功・熊丸敏博, 他7名
    • Journal Title

      日本醸造協会誌 99巻・2号

      Pages: 134-144

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 心白を持つ胚乳変異体米の粘度及び糊化特性と清酒醸造試験2004

    • Author(s)
      荒巻功・熊丸敏博, 他9名
    • Journal Title

      日本醸造協会誌 99巻・3号

      Pages: 189-201

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A modified TILLING system for rice mutant screening

    • Author(s)
      Suzuki T., M.Eguchi, H.Satoh, T.Kumamaru, N.Kurata
    • Journal Title

      Rice Genetice Newsletter 22(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Inheritance mode of Glup5 gene and the genetic relationships with other 57H mutant genes

    • Author(s)
      Y.Ueda, H.Satoh, M.Sato, Y.Takemoto, T.Kumamaru, M.Ogawa
    • Journal Title

      Rice Genetics Newsletter 21(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel 57H mutant gene, glup7, located on chromosome 4

    • Author(s)
      Y.Ueda, A.Sugino, Y.Takemoto, M.Sato, T.Kumamaru, M.Ogawa, H.Satoh
    • Journal Title

      Rice Genetics Newsletter 21(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Presentation] 高システインイネプロラミンの生合成・集積に関わる遺伝子のマッピング2008

    • Author(s)
      松坂 弘明, ら
    • Organizer
      日本育種学会
    • Place of Presentation
      明治大学
    • Year and Date
      2008-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mapping of genes for Cysteine-rich prolamin biosynthesis and accumulation in rice2008

    • Author(s)
      Matsusaka, H
    • Organizer
      Meeting of Japanese Society of Breeding
    • Place of Presentation
      Meiji University
    • Year and Date
      2008-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネ種子貯蔵蛋白質の分別集積・輸送におけるPDIL1-1およびPDIL2-3の機能解析2008

    • Author(s)
      恩田 弥生, ら
    • Organizer
      日本農芸化学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Function of PDIL1-1 and PDIL2-3 in sorting the storage proteins in rice endosperm2008

    • Author(s)
      Onda, Y
    • Organizer
      Annual meeting of Japan Society of Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Nagoya University
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネプロラミンPBの核構造形成における10kDaプロラミンの役割2008

    • Author(s)
      長嶺 愛, ら
    • Organizer
      日本植物生理学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2008-03-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Role of 10kDa prolamin in the formation of core structure in prolamin-PB of rice2008

    • Author(s)
      Nagamine, A
    • Organizer
      Annual meting of Japanese society of Plant Physiologists
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2008-03-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネプロラミンPBの核構造形成における10kDaプロラミンの役割2008

    • Author(s)
      長嶺愛, 小川雅広, 松阪弘明, 牛島智一, 佐藤光, 熊丸敏博
    • Organizer
      日本植物生理学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Mutation screening system for any genes in rice Establishment of a simplified TILLING method using efficiently mutagenized population2007

    • Author(s)
      Suzuki, T
    • Organizer
      Meeting of Japanese Society of Breeding
    • Place of Presentation
      Ehime University
    • Year and Date
      2007-09-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic analyses of prolamin mutation on Brewer's rice Yamadanishiki2007

    • Author(s)
      Matsusaka, H
    • Organizer
      Meeting of Japanese Society of Breeding
    • Place of Presentation
      Ehime University
    • Year and Date
      2007-09-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 中国在来イネ品種におけるプロラミン分子種の多様性2007

    • Author(s)
      張 効梅, ら
    • Organizer
      日本育種学会
    • Place of Presentation
      茨城大学
    • Year and Date
      2007-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネ種子における40kDプロティンジスルフィドイソメラーゼ発現解析2007

    • Author(s)
      劉 彩虹, ら
    • Organizer
      日本育種学会
    • Place of Presentation
      茨城大学
    • Year and Date
      2007-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diversity of prolamin polypeptides in Chinese local rice varieties2007

    • Author(s)
      Zhang, X
    • Organizer
      Meeting of Japanese Society of Breeding
    • Place of Presentation
      Ibaraki University
    • Year and Date
      2007-03-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The expreesion analysis of 40kDa protein disulfide isomerase in rice.2007

    • Author(s)
      Liu
    • Organizer
      Meeting of Japanese Society of Breeding
    • Place of Presentation
      Ibaraki University
    • Year and Date
      2007-03-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネ全遺伝子の突然変異検索法-高頻度変異系統群と簡易TILLING法の確立2006

    • Author(s)
      鈴木 温, ら
    • Organizer
      日本育種学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2006-09-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 酒米品種「山田錦」が有するプロラミン変異の遺伝解析2006

    • Author(s)
      松坂 弘明, ら
    • Organizer
      日本育種学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2006-09-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Springer2007

    • Author(s)
      Kumamaru, T., et. al.
    • Publisher
      Protein body biogenesis in cereal endosperms.In Endosperm-Development and Molecular Biology, O.A.Olsen, ed.Plant Cell Monographs(8)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Protein body biogenesis in cereal endosperms. In Endosperm-Development and Molecular Biology, O.-A.Olsen, ed.Plant Cell Monographs(8)2007

    • Author(s)
      Kumamaru, T., et al.
    • Total Pages
      18
    • Publisher
      Springer
    • Related Report
      2007 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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