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Functional analysis of NB-LRR gene family for plant immunity

Research Project

Project/Area Number 16380031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI Hideki  Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院農学研究科, 助教授 (20197164)

Co-Investigator(Kenkyū-buntansha) HASE Shu  Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院農学研究科, 助手 (10261497)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥9,700,000 (Direct Cost: ¥9,700,000)
KeywordsResistance gene / Hypersensitive response / Signal transduction / Cucumber mosaic virus / Plant / キュウリモザイクテスト
Research Abstract

We have isolated Cucumber mosaic virus (CMV) resistance gene RCY1 encoding coiled coil (CC), nucleotide binding site (NB) and leucine-rich repeat (LRR) domain-containing protein. Arabidopsis genome contains 〜200 member of RCY1 paralogs encoding NB-LRR protein. In this project, the function of RCY1 and other NB-LRR genes for the resistance to CMV was analyzed using a serious of RCY1 mutants and NB-LRR custom array. The obtained results suggested that (1)all CC, NB and LRR domains are required for complete resistance to CMV, (2)the resistance to CMV is dependent on the level of RCY1 expression, (3)RCY1 separately regulates development of hypersensitive cell death and restriction of virus spread. Furthermore, global analysis of 〜200 members of NB-LRR gene family in CMV resistance using custom array indicated that expression of five NB-LRR genes : At1g72890,At1g72900,At1g72910,At1g72930,At1g72940, which tandemly located on the chromosome I, was up-regulated in CMV resistance. The CMV-inducible R-like gene cluster was named "CIR1". To further analyze the function of CIR1 for CMV resistance, we transformed CIR1 cDNA under the control of CaMV35S promoter into A. thaliana carrying RCY1. The response of the obtained transformants to CMV will be extensively analyzed. The information obtained from this research will give a new insight to study the role of NB-LRR gene family for CMV resistance and molecular basis of virus resistance in plants.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (42 results)

All 2007 2006 2005 2004

All Journal Article (35 results) Book (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Up-regulation of LeATL6 that encodes a fungal elicitor-responsive ubiquitin ligase induces jasmonic acid-dependent proteinase inhibitor gene expression in tomato.2007

    • Author(s)
      Hondo, D. et al.
    • Journal Title

      Molecular Plant-Microbe Interactions 20

      Pages: 72-81

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of a novel Cys_2/His_2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco.2007

    • Author(s)
      Mitsuya, Y. et al.
    • Journal Title

      Journal of Plant Physiology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of LeA7L6 that encodes a fungal elicitor-responsive ubiquitin ligase induces jasmonic acid-dependent proteinase inhibitor gene expression in tomato.2007

    • Author(s)
      Hondo, D., Hase, S., Kanayama, Y., Yoshikawa, N., Takenaka, S., Takahashi, H.
    • Journal Title

      Molecular Plant-Microbe Interactions 20

      Pages: 72-81

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of a novel CYs_2/His_2-type zinc-finger protein as a component of a spemine-signaling pathway in tobacco.2007

    • Author(s)
      Mitsuya, Y., Takahashi, Y., Uehara, Y., Barberich, T., Miyazaki, A., Takahashi, H., Kusano, T.
    • Journal Title

      Journal of Plant Physiology (m press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of LeATL6 that encodes a fungal elicitor-responsive ubiquitin ligase induces jasmonic acid-dependent proteinase inhibitor gene expression in tomato.2007

    • Author(s)
      Hondo, D., et al.
    • Journal Title

      Molecular Plant-Microbe Interactions 20

      Pages: 72-81

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of a novel Cys_2/His_2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco.2007

    • Author(s)
      Mitsuya, Y., et al.
    • Journal Title

      Journal of Plant Physiology (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death.2006

    • Author(s)
      Sekine, K.-T.et al.
    • Journal Title

      Plant Molecular Biology 62

      Pages: 669-682

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Beta-cyanoalanine synthase as a molecular marker induced resistance by fungal glycoprotein elicitor and commercial plant activators.2006

    • Author(s)
      Takahashi, H. et al.
    • Journal Title

      Phytopathology 96

      Pages: 908-916

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of transient ethylene and reduction in severity of tomato bacterial wilt by Pythium oligandrum.2006

    • Author(s)
      Hase, S. et al.
    • Journal Title

      Plant Pathology 55

      Pages: 537-543

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related genes in sugar beet.2006

    • Author(s)
      Takenaka, S. et al.
    • Journal Title

      Molecular Plant Pathology 7

      Pages: 325-339

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Single amino acid alterations in Arabidosis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death.2006

    • Author(s)
      Sekine, K.-T., Ishihara, T., Hase, S., Kusano, T., Shah, J., Takahashi, H.
    • Journal Title

      Plant Molecular Biology 62

      Pages: 669-682

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Beta-cyanoalanine synthase as a molecular marker induced resistance by fungal glycopotein elicitor and commercial plant activators.2006

    • Author(s)
      Takahashi, H., Ishihara, T., Hase, S., Chiba, A., Nakaho, K., Arie, T., Teraoka, T., Iwata, M., Tugane, T., Shibata, D., Takenaka, S.
    • Journal Title

      Phytopathology 96

      Pages: 908-916

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of transient ethylene and reduction in severity of tomato bacterial wilt by Pythium oligandrum.2006

    • Author(s)
      Hase, S., Shimizu, A., Nakaho, K., Takenaka, S., Takahashi, H.
    • Journal Title

      Plant Pathology 55

      Pages: 537-543

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related gars in sugar beet2006

    • Author(s)
      Takenaka, S., Nakamura, Y., Ono, T., Sekiguchi, H., Masunaka, A., Takahashi, H.
    • Journal Title

      Molecular Plant Pathology 7

      Pages: 325-339

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death.2006

    • Author(s)
      Sekine, K.-T., et al.
    • Journal Title

      Plant Molecular Biology 62

      Pages: 669-682

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related genes in sugar beet.2006

    • Author(s)
      Takenaka, S., et al.
    • Journal Title

      Molecular Plant Pathology 7

      Pages: 325-339

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Beta-cyanoalanine synthase as a molecular marker induced resistance by fungal glycoprotein elicitor and commercial plant activators.2006

    • Author(s)
      Takahashi, H., et al.
    • Journal Title

      Phytopathology 96

      Pages: 908-916

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Catalog of Micro-Tom tomato responses to common fungal, bacterial and viral pathogens.2005

    • Author(s)
      Takahashi, H. et al.
    • Journal Title

      Journal of General Plant Pathology 71

      Pages: 8-22

    • NAID

      10014471856

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Tobacco ZFT1, a transcriptional repressor with a Cys_2/His_2 type zinc finger motif that functions in spermine-signaling pathway.2005

    • Author(s)
      Uehara, Y. et al.
    • Journal Title

      Plant Molecular Biology 59

      Pages: 433-446

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment.2005

    • Author(s)
      Zheng, M.S.et al.
    • Journal Title

      Plant Science 168

      Pages: 415-422

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Catalog of Micro-Tom tomato responses to common fungal, bacterial and viral Pathogens.2005

    • Author(s)
      Takahashi, H., Shimizu, A., Arie, T., Rosmalawati, S., Fukushima, S., Kikuchi, M., Hikichi, Y., Kanda, A., Ohnishi, K., Ichinose, Y., Yasuda, C., Kodama, M., Egusa, M., Masuta, C., Sawada, H., Shibata, D., Hori, K., Watanabe, Y.
    • Journal Title

      Joumal of General Plant Pathology 71

      Pages: 8-22

    • NAID

      10014471856

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tobacco ZFT1, a transcriptional repressor with a Cys_2/His_2 type zinc finger motif that functions in in spermine-signaling pathway.2005

    • Author(s)
      Uehara, Y., Takahashi, Y., Berberich, T., Miyazaki, A., Takahashi, H., Matsui, K., Ohme-Takagi, M., Saitoh, H., Terauchi, R., Kusano, T.
    • Journal Title

      Plant Molecular Biology 59

      Pages: 433-446

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of the cis-acting dements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment.2005

    • Author(s)
      Zheng, M.S., Takahashi, H., Miyazaki, A., Yamaguchi, K., Kusano, T.
    • Journal Title

      Plant Science 168

      Pages: 415-422

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antagonistic interactions between the SA- and JA- signaling pathways in Arabidopsis modulate expression of defense genes and gene-for-gene resistance to Cucumber mosaic virus.2004

    • Author(s)
      Takahashi, H. et al.
    • Journal Title

      Plant Cell Physiology 45

      Pages: 803-809

    • NAID

      10013129021

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhanced resistance to Cucumber mosaic virus in the Arabidopsis thalianassi2 mutant is mediated via an SA-independent mechanism.2004

    • Author(s)
      Sekine, K.-T.et al.
    • Journal Title

      Molecular Plant-Microbe Interaction 17

      Pages: 623-632

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with Cucumber mosaic virus.2004

    • Author(s)
      Ishihara, T. et al.
    • Journal Title

      Plant Cell Physiology 45

      Pages: 470-480

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signaling pathways that differ in salicylate involvement.2004

    • Author(s)
      Zheng, M.S.et al.
    • Journal Title

      Planta 218

      Pages: 740-750

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antagonistic interactions between the SA-and IA-signaling pathways in Arabidopsis modulate expression of defense genes and gene-for-gene resistance to Cucumbermasaicvirus2004

    • Author(s)
      Takahashi, H, Kanayama, Y., Zheng, M.S., Kusano, T., Hase, S., Ikegami, M., Shah, S.
    • Journal Title

      Plant Cell Physiology 45

      Pages: 803-809

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhanced resistance to Cucumber mosaic virus in the Anabidopsis thaliana ssi2 mutant is mediated via an SA-independent mechanism.2004

    • Author(s)
      Sekine, K.-T., Nandi, A., Ishihara, T., Hase, S., Ikegami, M., Shah, S., Takahashi, H.
    • Journal Title

      Molecular Plant-Microbe Interaction 17

      Pages: 623-632

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with Cucumber mosaic virus.2004

    • Author(s)
      Ishihara, T., Sakurai, N., Sekine, K.-T., Hase, S., Ikegami, M., Shibata, D., Takahashi, H.
    • Journal Title

      PlantCell Physiology 45

      Pages: 470-480

    • NAID

      10012852556

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signaling pathways that differ in salicylate involvement2004

    • Author(s)
      Zheng, M.S., Takahashi, H., Miyazaki, A., Hamamoto, H., Shah, J., Yamaguchi, I., Kusano, T.
    • Journal Title

      Planta 218

      Pages: 740-750

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antagonistic interactions between the SA- and JA- signaling pathways in Arabidopsis modulate expression defense genes and gene-for-gene resistance to Cucumber mosaic virus.2004

    • Author(s)
      Takahashi et al.
    • Journal Title

      Plant Cell Physiology 45

      Pages: 803-809

    • NAID

      10013129021

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The enhanced resistance to Cucumber mosaic virus in the Arabidopsis thaliana stearoyl-ACP desaturase-deficient ssi2 mutant is mediated by an SA-independent, but EDS5-dependent signaling mechanism.2004

    • Author(s)
      Sekine et al.
    • Journal Title

      Molecular Plant-Microbe Interaction 17

      Pages: 623-632

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with Cucumber mosaic virus.2004

    • Author(s)
      Ishihara et al.
    • Journal Title

      Plant Cell Physiology 45

      Pages: 470-480

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment.2004

    • Author(s)
      Zheng et al.
    • Journal Title

      Plant Science 168

      Pages: 415-422

    • Related Report
      2004 Annual Research Report
  • [Book] 「ウイルス感染に対する宿主応答」作物保護の新展開-バイオサイエンスのかけはし- (羽柴輝良 監修)2005

    • Author(s)
      高橋英樹
    • Total Pages
      338
    • Publisher
      ソフトサイエンス社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] "Signal transduction pathways for the resistance response to Cucumber mosaic virus." Genomic and Genetic Analysis of Plant Parasitism and Defense. (Eds. S. Tsuyumu, J. Leach, T. Shiraishi and T. Walpert)2005

    • Author(s)
      Takahashi, H. et al.
    • Total Pages
      301
    • Publisher
      APS Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Response of host plants to virus infection, New insight of Plant Protection (edited by Hashiba, T.)(in Japanese)2005

    • Author(s)
      Takahashi, H.
    • Total Pages
      338
    • Publisher
      Softscience-sha
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Signal transduction pathways for the resistance response to Cucumber mosaic virus. Genomic and Genetic Analysis of Plant Parasitism and Defense (Eds.S.Tsuyumu, J.Leach, T.Shiraishi and T.Walpert)2005

    • Author(s)
      Takahashi, H., Sekine, K.-T., Ishihara, T., Hase, S., Ikegami, M., Shah, J.
    • Total Pages
      301
    • Publisher
      APS Press, St Paul, Minnesota, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 「ウイルスに対する宿主抵抗性」新版 分子レベルからみた植物の耐病性 植物細胞工学シリーズ19(島本 功・渡辺雄一郎・柘植尚志 監修)2004

    • Author(s)
      高橋英樹
    • Total Pages
      214
    • Publisher
      秀潤社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Resistance to virus in host plants, Disease resistance in plants at the molecular level (edited by Shimamoto, K., Watanabe, Y.and Tsuge, T.) Plant Biotechology 19(in Japanese)2004

    • Author(s)
      Takahashi, H.
    • Total Pages
      214
    • Publisher
      Shuujyunnsha
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 植物病原体に対する誘導抵抗性の指標となるマーカー遺伝子の単離2005

    • Inventor(s)
      高橋英樹, 竹中重仁
    • Industrial Property Rights Holder
      東北大学・(独)農業・生物系産業技術研究機構
    • Industrial Property Number
      2005-353502
    • Filing Date
      2005-12-07
    • Acquisition Date
      2005-12-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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