Mechanisms of disease symptom expression by potexviruses

• Project/Area Number: 16380033
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: The University of Tokyo
• Principal Investigator: UGAKI Masashi The University of Tokyo, Graduate School of Science, Professor, 大学院新領域創成科学研究科, 教授 (20323438)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥7,800,000 (Direct Cost: ¥7,800,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000); Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000); Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
• Keywords: Potexvirus / Potato virus X / Plantago asiatica mosaic virus / symptom / ポテックスウイルス / モザイク / 輪状斑 / 植物ウイルス

Research Abstract

Upon infection to plant hosts, plant viruses cause vast varieties of disease symptoms including mosaic, yellows, chlorosis, ring spot, mottle, and so on. Molecular mechanisms underlying expression of these symptoms, however, have scarcely been uncovered. In this project, I studied the mechanism using two species of plant viruses belonging to Genus Potexvirus. Potato virus X (PVX) and Plantago asiatica mosaic virus (PIAMV). The strains OS and BH of PVX cause ring spot and mosaic, respectively, upon infection to tobacco plant (Nicotiana tabacum). Construction of chimeric genomes of the two strains and site-directied mutagenesis of the viral genomes revealed that the 58th nucleotide of the 5 prime untranslated region of the viral genome was responsible for determination of distinct symptoms. Distribution of viral RNA of each PVX strain, as well as transcription of host defense genes, was monitored in the infected leaves by in situ hybridization technique. In the area of ring spot, some plant genes related to the hypersensitive response (HR) such as pathogenesis-related protein 1a (PR-1a) were expressed. Viral RNA was found both inside and outside of the area of ring spot. These data suggest that the ring spot symptom caused by strain OS is a result of induced HR-like plant cell death yet unsuccessful in restricting the spread of the virus. The strains Li1 and Li6 of PlAMV cause systemic necrosis and mosaic, respectively, upon infection to Nicotiana benthamiana plants. Molecular analysis revealed that the 1154th amino acid of the viral replicase encoded in the PlAMV genome was responsible for the symptom determination. HR-like response was also observed in plants infected with the strain Li1. These data support the idea that some symptoms such as ring spot and systemic necrosis are caused by host plant responses which tried to restrict viral spread.

Research Products

• [Journal Article] A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis (2006)
  • Author(s): Ozeki, J., Takahashi, S., Komatsu, K., Kagiwada, S., Yamashita, K., Mori, T., Hirata, H., YamaJi, Y., Ugaki, M., Namba, S.
  • Journal Title: Archives of Virology 151 Pages: 2067-2075
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The efficiency of interference of Potato virus X infection depends on the target gene. (2006)
  • Author(s): Takahashi, S., Komatsu, K., Kagiwada, S., Ozeki, J., Mori, T., Hirata, H., Yamaji, Y., Ugaki, M., Namba, S.
  • Journal Title: Virus Research 116 Pages: 214-217
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis (2006)
  • Author(s): Ozeki, J., Takahashi, S., Komatsu, K., Kagiwada, S., Yamashita, K., Mori, T., Hirata, H., Yamaji, Y., Ugaki, M., Namba, S.
  • Journal Title: Archives of Virology 151 Pages: 2067-2075
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The efficiency of interference of Potato virus X infection depends on the target gene (2006)
  • Author(s): Takahashi et al.
  • Journal Title: Virus Research 116 Pages: 214-217
• [Journal Article] A single amino acid residue of RNA-dependent RNA polymerase in the Potato virus X genome determines the symptoms in Nicotiana plants. (2005)
  • Author(s): Kagiwada, S., Yamaji, Y., Komatsu, K., Takahashi, S., Mori, T., Hirata, H., Suzuki, M., Ugaki, M., Namba, S.
  • Journal Title: Virus Research 110 Pages: 177-182
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely related Japanese strains of Potato virus X. (2005)
  • Author(s): Komatsu, K., Kagiwada, S., Takahashi, S., Mori, T., Yamaji, Y., Hirata, H., Ozeki, J., Yoshida, A., Suzuki, M., Ugaki, M., Namba, S.
  • Journal Title: Virus Genes 31 Pages: 99-105
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely related Japanese strains of Potato virus X. (2005)
  • Author(s): Komatsu, K., Kagiwada, S., Takahashi, S., Mori, T., Yamaji, Y., Hirata, H., Ozeki, J., Yoshida, A., Suzuki, M., Ugaki, M.Namba, S.
  • Journal Title: Virus Genes 31 Pages: 99-105
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A single amino acid residue of RNA-dependent RNA polymerase in the Potat virus X genome determines the symptoms in Nicotiana plants. (2005)
  • Author(s): Kagiwada et al.
  • Journal Title: Virus Research 110 Pages: 177-182
• [Journal Article] Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely related Japanese strains of Potato virus X (2005)
  • Author(s): Komatsu et al.
  • Journal Title: Virus Genes 31 Pages: 99-105
• [Journal Article] Secretion of immunodominant membrane protein from onion yellows phytoplasma through the Sec protein-translocation system in Escherichia coli (2004)
  • Author(s): Kakizawa, S. et al.
  • Journal Title: Microbiology 150 Pages: 135-142
• [Journal Article] In planta dynamic analysis of onion yellows phytoplasma using localized inoculation by insect transmission (2004)
  • Author(s): Wei, W.et al.
  • Journal Title: Phytopathology 94 Pages: 244-250
• [Journal Article] An antibody against the SecA membrane protein of one phytoplasma reacts with those of phylogenetically different phytoplasmas (2004)
  • Author(s): Wei, W.et al.
  • Journal Title: Phytopathology 94 Pages: 683-686