Identification of an avirulence gene island in Pyriculazia chromosomes and analyses of its transposition and disorganization
| Project/Area Number |
16380036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Kobe University |
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Principal Investigator |
TOSA Yukio Kobe University, Graduate School of Science and Technology, Associate Professor, 自然科学研究科, 助教授 (20172158)
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| Co-Investigator(Kenkyū-buntansha) |
MAYAMA Shigeyuki Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (00112251)
NAKAYASHIKI Hitoshi Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50252804)
PARK Pyoyun Kobe University, Graduate School of Science and Technology, Professor, 自然科学研究科, 教授 (20147094)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2005: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 2004: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | Magnaporthe / Pyricularia / avirulence gene |
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| Research Abstract |
The significance of AVR1-CO39, an avirulence gene of the blast fungus corresponding to Pi-CO39(t) in rice cultivars, during the evolution and differentiation of the blast fungus was evaluated by studying its function and distribution in Pyricularia spp. When the presence/absence of AVR1-CO39 was plotted on a dendrogram constructed from rDNA sequences, a perfect parallelism was observed between its distribution and the phylogeny of Pyricularia isolates. AVR1-CO39 homologs were exclusively present in one species, P.oryzae, suggesting that AVR1-CO39 appeared during the early stage of evolution of P.oryzae. Transformation assays showed that all the cloned homologs tested are functional as an avirulence gene, indicating that selection has maintained their function. Nevertheless, Oryza isolates (isolates virulent on Oryza spp.) in P. oryzae were exceptionally non-carriers of AVR1-CO39. All Oryza isolates suffered from one of the two types of known rearrangements at the Avr1-CO39 locus, i.e., G type and J type. These types were congruous to the two major lineages of Oryza isolates from Japan determined by MGR586 and MAGGY. These results indicate that AVR1-CO39 was lost during the early stage of evolution of the Oryza-specific subgroup of P.oryzae. Interestingly, its corresponding resistance gene, Pi-CO39(t) is not widely distributed in Oryza spp.
Homologs of AVR-Pita, an avirulence gene corresponding to Pita, were widely distributed not only in P.oryzae, but also in P.grisea and P.spp. Interestingly, the chromosomes with the homologs were different from isolates to isolates. Analyses of flanking regions of these homologs suggested that the insertion of transposable elements into their flanking regions gave them mobility.
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