Basic Research on the Development of an Artificial Root Nodule for a Novel Nitrogen Fixation System.

• Project/Area Number: 16380048
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: National University Corporation Tokyo University of Agriculture and Technology
• Principal Investigator: YOKOYAMA Tadashi National University Corporation Tokyo University of Agriculture and Technology, Institute of symbiotic science and technology, associate professor, 大学院・共生科学技術研究部, 助教授 (70313286)
• Co-Investigator(Kenkyū-buntansha): ARIMA Yasuhiro National University Corporation Tokyo University of Agriculture and Technology, Institute of symbiotic science and technology, Professor, 大学院・共生科学技術研究部, 教授 (90011973)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥13,200,000 (Direct Cost: ¥13,200,000); Fiscal Year 2005: ¥4,900,000 (Direct Cost: ¥4,900,000); Fiscal Year 2004: ¥8,300,000 (Direct Cost: ¥8,300,000)
• Keywords: soybean / root nodule / Bradyrhizobium / symbiosome / bacteroid / symbiosis / biological nitrogen fixation / rhizobium / 静菌 / マクロアレイ膜 / Peri Bacteroid Space

Research Abstract

Bradyrhizobium is a symbiont of soybean. Bradyrhizobium cells differentiate into bacteroids in soybean root nodule cells and start nitrogen fixation. However, a molecular mechanism of the differentiation from free living cell to bacteroid in root nodule cells is stll unknown. The bacteroids enclose with PBS (peri-bacteroidal space)-solution in symbiosome. The PBS-solution expects to have several plant signals involving in deformation processes from rhizobium to bacteroids or maintenance of bacteroids. We compared profiles of gene expressions between B.japonicum USDA 110 with and without the PBS-solution isolated from soybean root nodules in terms of analysis by macroarray containing 3960 DNA fragments which covered with a whole genome of B.japonicum USDA 110. At 2 hrs incubation of USDA 110 with the PBS-solution, transcriptional levels of 151 DNA fragment regions were up-regulated more than three times in contrast with those in USDA 110 without the PBS-solution. A transcriptional level of a DNA region designated as BJ7062 showed nine-thousand times higher than that in untreated cell. We also confirmed up-regulation of two genes of nifB and nifN in USDA 110 cells with the PBS-solution. Furthermore, we re-confirmed up-regulation traits of these genes using RT-PCR method. Transcriptional levels of forty-three DNA regions in USDA 110 cells with the PBS-solution were down-regulated less than one-third in contrast with those of USDA 110 without the PBS-solution. We also found several Large transcriptional repression DNA regions in USDA 110 cells with the PBS-solution and their location of the transcriptional repression DNA regions were identical with those in bacteroids of soybean root nodules with USDA 110 at 5 weeks. These observations indicate PBS -solution in symbiosome obtained from soybean root nodules has several unknown plant factors to elicit or repress gene expressions of B.japonicum USDA 110.

Research Products

• [Journal Article] ダイズ根粒シンビオソームのPeri-bacteroid spaceに存在する溶液を処理することで単生ダイズ根粒菌USDA110株に誘導される遺伝子応答の網羅的解析 (2006)
  • Author(s): 横山 正, 市田沙智子, 大石真子, 中坪知史, 手島光平, 有馬泰紘, 大和田琢二, 南澤 究, 三井久幸, 板倉 学, 金子貴一, 田畑哲之, 佐伯和彦, 大森博文, 田島茂行, 内海俊樹, 阿部美紀子
  • Journal Title: 植物微生物研究会第15回研究交流会講演要旨集ISSN1341-0652 Vol. 15 Pages: 5-6
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comprehensive analysis of plant gene expression in soybean root nodules at different growth stages. (2005)
  • Author(s): Kaewsuralikhit S., Yokoyama T., Kouchi H., Arima Y.
  • Journal Title: Soil Sci Plant Nutr. Vol. 51 Pages: 313-322
  • NAID: 110004704772
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 根粒PBS物質投与により引き起こされるダイズ根粒菌タンパク質2次元電気泳動像の変化 (2005)
  • Author(s): 大石真子, 横山 正, 有馬泰紘
  • Journal Title: 日本土壌肥料学会講演要旨集ISSN0288-5840 Vol. 51 Pages: 51-51
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] ダイズ根粒のperibacteroid apaceに存在する単生根粒菌増殖抑制物質について (2005)
  • Author(s): 市田沙智子, 横山 正, 有馬泰紘
  • Journal Title: 日本土壌肥料学会講演要旨集ISSN0288-5840 Vol. 51 Pages: 56-56
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comprehensive analysis of plant gene expression in soybean root nodules at different growth stages. (2005)
  • Author(s): Kaewsuralikhit S., Yokoyama T., Kouchi H., Arima Y.
  • Journal Title: Soil Sci Plant Nutr. Vol.51 Pages: 313-322
  • NAID: 110004704772
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genome-wide Vie of Mesorhizobium and Bradyrhizobium, expression island clustered on symbiosis island and genome diversity. (2005)
  • Author(s): Uchiumi T., Ohwada T., Kaneko T., Yokoyama T., Saeki K., Hayashi M., Tajima S., Minamizawa K.
  • Journal Title: Current Plant Science and Biotechnology in Agriculture. Vol.41 Biological Nitrogen Fixation, Sustainable Agriculture and the Environment. (Edited by Y.Wang, M.Lin, Z.Tian, C.Elmerich, and W.Newton) (Springer, Netherlands.) ISBN-101-4020-3569-1 Pages: 139-139
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Global gene expression in Bradyrhizobium japonicum USDA 110 induced by PBS-solution isolated from peri-bacteroidal spaces in symbiosomes of soybean root nodules in terms of macroarray analysis. (2005)
  • Author(s): Yokoyama T., Ichida S., Ohishi N., Nakatsubo S., Tejima K., Arima Y., Ohwada T., Minamisawa K., Mitsui H., Itakura M., Kaneko T., Tabata S., Saeki K., Omori H., Tajima S., Uchiumi T., Abe M.
  • Journal Title: Abstract of the 15^<th> Annual Meeting for plant-Microbe Interactions Vol.15, ISSN1341-0652 Pages: 4-5
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Image analysis of cell proteins of Bradyrhizobium japonicum USDA 110 incubated with symbiosome solution isolated from soybean root nodules in terms of high-resolution 2D electrophoresis. (2005)
  • Author(s): Ooishi N., Yokoyama T., Arima Y.
  • Journal Title: Abstract of the annual meeting for Japanese society of soil science and plant nutrition Vol.51 Pages: 51-51
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth inhibitor of Bradyrhizobium in symbiosome solution isolated from soybean root nodules. (2005)
  • Author(s): Ichida S., Yokoyama T., Arima Y.
  • Journal Title: Abstract of the annual meeting for Japanese society of soil science and plant nutrition Vol.51 Pages: 56-56
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] ダイズ根粒シンビオソームのPeri-bacteroid spaceに存在する溶液を処理することで単生ダイズ根粒菌USDA110株に誘導される遺伝子応答の網羅的解析 (2005)
  • Author(s): 横山 正, 市田紗智子, 大石真子, 中坪知史, 手島光平, 有馬泰紘, 大和田琢二, 南澤 究, 三井久幸, 板倉 学, 金子貴一, 田畑哲之, 佐伯和彦, 大森博文, 田島茂行, 内海俊樹, 阿部美紀子
  • Journal Title: 植物微生物研究会 第15回研究交流会講演要旨集ISSN1341-0652 Vol.15 Pages: 5-6
• [Journal Article] Comprehensive analysis of plant gene expression in soybean root nodules a different growth stages. (2005)
  • Author(s): Kaewsuralikhit S., Yokoyama T., Kouchi H., Arima Y.
  • Journal Title: Soil Sci Plant Nutr. Vol.51 Pages: 313-322
  • NAID: 110004704772
• [Journal Article] 根粒PBS物質投与により引き起こされるダイズ根粒菌タンパク質2次元電気泳動像の変化 (2005)
  • Author(s): 大石真子, 横山 正, 有馬泰紘
  • Journal Title: 日本土壌肥料学会 講演要旨集 Vol.51 Pages: 51-51
• [Journal Article] ダイズ根粒のperibacteroid apaceに存在する単生根粒菌増殖抑制物質について (2005)
  • Author(s): 市田紗智子, 横山 正, 有馬泰紘
  • Journal Title: 日本土壌肥料学会 講演要旨集 Vol.51 Pages: 56-56
• [Journal Article] Expression Island Cluster on the Symbiosis Island of the Mesorhizobium loti Genome. (2004)
  • Author(s): Uchiumi T., Ohwada T., Itakura M., Mitui H., Nukui N., Dawadi P., Kaneko T., Tabata S., Yokoyama T., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa T., Sriprang R., Murooka Y., Tajima S., Simomura K., Nomura M., Suzuki A., shimoda Y., Sioya K., Abe M., Minamisawa K
  • Journal Title: J. of Bacteriol. Vol. 186 Pages: 2439-2448
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] ダイズ根粒PBS物質が単生状態のBradyrhizobium japonicum USDA110株の増殖遺伝子発現に及ばす影響 (2004)
  • Author(s): 市田沙智子, 横山 正, 有馬泰紘
  • Journal Title: 日本土壌肥料学会講演要旨集ISSN0288-5840 Vol. 50 Pages: 61-61
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression Island Cluster on the Symbiosis Island of the Mesorhizobium lote Genome. (2004)
  • Author(s): Uchiumi T., Ohwada T., Itakura M., Mitui H., Nukui N., Dawadi P., Kaneko T., Tabata S., Yokoyama T., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa T., Sriprang R., Murooka Y., Tajima S., Simomura K., Nomura M., Suzuki A., shimoda Y., Sioya K., Abe M., Minamisawa K.
  • Journal Title: J.of Bacteriol. Vol.186 Pages: 2439-2448
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Large-Scale Analysis of Gene Expression Profiles during Early Stages of Root Nodule Formation in a Model Legume, Lotus japonicus. (2004)
  • Author(s): Kouchi H. 他
  • Journal Title: DNA Research 11 Pages: 263-274
• [Journal Article] Expression Islands Clustered on the Symbiosis Island of the Mesorhizobium loti Genome. (2004)
  • Author(s): Uchiumi, T. 他
  • Journal Title: Journal of Bacteriology 186 Pages: 2439-2448
• [Journal Article] Praparetion and Biological Activity of Molecular Probes to Identify and Analyze Jasmonic Acid-binding Protein. (2004)
  • Author(s): Jikumaru Y. 他
  • Journal Title: Biosci.Biotechnol.Biochem. 98 Pages: 1461-1466
• [Journal Article] ダイズ根粒PBS物質が単生状態のBradyrhizobium japonicum USDA110株の増殖及び遺伝子発現に及ぼす影響 (2004)
  • Author(s): 市田紗智子, 横山正, 有馬泰紘
  • Journal Title: 日本土壌肥料学会講演要旨集 50 Pages: 61-61
• [Book] Current Plant Science and Biotechnology in Agriculture. Vol. 41 Biological Nitrogen Fixation, Sustainable Agriculture and the Environment. Edited by Y. Wang, M. Lin, Z. Tian, C. Elmerich, and W. Newton, ISBN-101-4020-3569-1, Springer, Netherlands. (2005)
  • Author(s): Uchiumi T., Ohwada T., Kaneko T., Yokoyama T., Saeki K., Hayashi M., Tajima S., Minamizawa K.
  • Total Pages: 139
  • Publisher: Genome-wide Vie of Mesorhizobium and Bradyrhizobium, expression island clustered on symbiosis island and genome diversity.
  • Description: 「研究成果報告書概要(和文)」より