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Confirmation of multiple symbiotic systems of plant-Frankia-mycorrhiza with a higher stress tolerance and its application to the rehabilitation of degraded lands

Research Project

Project/Area Number 16380051
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

SASAKAWA Hideo  Okayama University, Graduate School of Natural Science and Technology, Professor (40115572)

Co-Investigator(Kenkyū-buntansha) KANZAKI Hiroshi  OKAYAMA UNIVERSITY, Graduate School of Natural Science and Technology, Professor (60183787)
YAMAMOTO Mikihiro  OKAYAMA UNIVERSITY, Graduate School of Natural Science and Technology, Associate Professor (60274015)
SHIMA Kazuto  OKAYAMA UNIVERSITY, Graduate School of Environmental Science, Associate Professor (80274017)
EZAWA Tatsuhiro  Hoddaido University, Graduate School of Agriculture, Associate Professor (40273213)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥13,010,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordsactinorhizal plants / Frankia / ectomycorrhiza / tripartite symbiosis / environmental stress tolerance / RFLP analysis / 多様性 / ストレス耐性 / 接種試験 / 多重共生系の構築 / オオバヤシャブシ / 生育促進 / 多重共生 / 系統解析 / 宿主領域
Research Abstract

This research was clone to establish a multiple symbiotic system of actinorhizal plant-Frankia- mychorrhiza with a higher environmental stress tolerance and to confirm it as an effective system for the recovery of the degraded lands. The results obtained were as follows.
1. One strain from the root nodules of Elaeagnus macrophylla and 4 strains from the root nodules of Myrica rubra were isolated and they were identified as Frankia by their morphological characteristics and inoculation test.
2. Frankia Apel strain was isolated from the root nodules of Alnus pendula growing in acid soil. The growth pH range of the strain was between 6.0 and 8.0, and the value was not different from those of Frankia which were isolated from the root nodules of actinorhizal plants growing in neutral pH.
3. Host range of each Frankia strain obtained by inoculation test and the result of phylogenic analysis with 16S rDNA sequence of each strain were well consistent.
4. Frankia strains reserved in the lab could be well discriminated by RFLP analysis of nifD-KIGS region and this method was effective for the determination of Frankia strain in the nodules of actinorhizal plants growing under natural conditions.
5. Pisolithus tinctorius or Cenococcum geophilum which had been isolated and reserved were inoculated with Frankia to 4 actinorhizal plants, but no mycorrhizal infection was observed.
6. One mycorrhiza-like fungus was isolated from 4 species of pine mycorrhizal mushrooms, respectively, and also one mycorrhiza-like fungus was isolated from mycorrhizal mushroom of Alnus sieboldiana. PCR-RFLP analyses strongly suggested that these fungi could be mycorrhizal fungi which infect on both pine and Alnus species.
These results gave us a prospect for the establishment of tripartite symbiotic system on actinorhizal plants which is effective for the recovery of the degraded lands

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (19 results)

All 2008 2007 2006 2005 2004

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (15 results)

  • [Journal Article] Host range of Frankia isolated from actinorhizal plants growing in Japan and their relatedness based on 16S rDNA.2008

    • Author(s)
      Yuki Nagashima, Chiharu Tani, Mikihiro Yamamoto, Hideo Sasakawa
    • Journal Title

      Soil Sci. Plant Nutr. 54

      Pages: 685-693

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Host range of Frankia strains isolated from actinorhizal plants growing in Japan and their relatedness based on 16S rDNA.2008

    • Author(s)
      Yuki Nagashima
    • Journal Title

      Soil Sci. Plant Nutr. 54(未決定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proline accumulates in Casuarina equisetifolia seedlings under salt stress.2006

    • Author(s)
      Chiharu Tani, Hideo Sasakawa
    • Journal Title

      Soil Sci. Plant Nutr. 52

      Pages: 21-25

    • NAID

      110006251000

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Proline accumulates in Casuarina equisetifolia seedlings under salt stress.2006

    • Author(s)
      Chiharu Tani
    • Journal Title

      Soil Sci.Plant Nutr. 52(1)

      Pages: 21-25

    • Related Report
      2005 Annual Research Report
  • [Presentation] 自然条件下で生育するアクチノリザル植物根粒内フランキアのRFLP解析2007

    • Author(s)
      長島由季, 増田宏之, 笹川英夫
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2007-09-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RFLP analysis of Frankia in the nodules of actinorhizal plants growing under natural conditions.2007

    • Author(s)
      Yuki Nagashima, Hiroyuki Masuda, Hideo Sasakawa
    • Organizer
      Annual Meeting of Japanese Society of Plant-Microbe Interaction
    • Place of Presentation
      Kagoshima City
    • Year and Date
      2007-09-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 分離源を異にするフランキア菌株の混合接種によって形成された根粒内菌株の同定と感染頻度2007

    • Author(s)
      長島由季
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農業大学(東京)
    • Year and Date
      2007-08-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 分離源を異にするフランキア菌株の混合接種によって形成された根粒内菌株の同定と感染頻度.2007

    • Author(s)
      長島由季, 増田宏之, 笹川英夫
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京
    • Year and Date
      2007-08-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of Frankia strains in the nodules for med by inoculation of mix-cultured Frankia and infection frequency of the Frankia strains.2007

    • Author(s)
      Yuki Nagashima, Hiroyuki Masuda, Hideo Sasakawa
    • Organizer
      Annual Meeting of Japanese Society of Soil Science and Plant Nutrition
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-08-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 酸性土壌に生育するハンノキ属植物からのフランキアの分離と分離菌の耐酸性2006

    • Author(s)
      増田宏之, 長島由季, 水野敦夫, 笹川英夫
    • Organizer
      植物微生物研究会
    • Place of Presentation
      札幌市
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] nifD-K IGS領域RFLP解析によるフランキア菌株の識別.2006

    • Author(s)
      長島由季, 高橋友香, 笹川英夫
    • Organizer
      植物微生物研究会
    • Place of Presentation
      札幌市
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation of Frankia from the root nodules of Alnus sp. in acid soil and acid tolerance of the isolates.2006

    • Author(s)
      Hiroyuki Masuda, Yuki Nagashima, Atsuo Mizuno, Hideo Sasakawa
    • Organizer
      Annual Meeting of Japanese Society of Plant-Microbe Interaction
    • Place of Presentation
      Sapporo City
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of Frankia strains base on RFLP analysis of the nifD-K IGS region.2006

    • Author(s)
      Yuki Nagashima, Yuka Takahashi, Hideo Sasakawa
    • Organizer
      Annual Meeting of Japanese Society of Plant-Microbe Interaction
    • Place of Presentation
      Sapporo City
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アクチノリザル植物根粒から分離されたフランキア株の生育pH範囲と培地pHの変化.2005

    • Author(s)
      長島由季, 水野敦夫, 谷千春, 笹川英夫
    • Organizer
      植物微生物研究会
    • Place of Presentation
      高松市
    • Year and Date
      2005-09-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Growth pH range of Frankia strains isolated from the root nodules of actinorhizal plants and pH change of the culture medium.2005

    • Author(s)
      Yuki Nagashima, Atsuo Mizuno, Chiharu Tani, Hideo Sasakawa
    • Organizer
      Sapporo City Annual Meeting of Japanese Society of Plant-Microbe Interaction
    • Place of Presentation
      Takamatsu City
    • Year and Date
      2005-09-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ヤマモモの雌雄判別に有効なRAPDマーカーの検索.2004

    • Author(s)
      金谷一平, 谷千春, 笹川英夫
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      福岡市
    • Year and Date
      2004-09-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Search of an effective RAPD marker for the determination of the sex of Myrica rubra.2004

    • Author(s)
      Ippei Kanaya, Chiharu Tani, Hideo Sasakawa
    • Organizer
      Annual Meeting of Japanese Society of Soil Science and Plant Nutrition
    • Place of Presentation
      Fukuoka City
    • Year and Date
      2004-09-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アクチノリザル植物根粒から分離したフランキア株の宿主領域と16S rDNAに基づく類縁関係.2004

    • Author(s)
      長島由季, 谷千春, 木谷圭詞, 山本幹博, 笹川英夫
    • Organizer
      植物微生物研究会
    • Place of Presentation
      東広島市
    • Year and Date
      2004-09-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Host range and relatedness base on 16S rDNA of Frankia strains isolated from the root nodules of actinorhizal plants.2004

    • Author(s)
      Yuki Nagashima, Chiharu Tani, Keiji Kitani, Mikihiro Yamamoto, Hideo Sasakawa
    • Organizer
      Annual Meeting of Japanese Society of Plant-Microbe Interaction
    • Place of Presentation
      Higashihiroshima City
    • Year and Date
      2004-09-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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