| Budget Amount *help |
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2004: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Research Abstract |
In this study, we focused on the carbon dioxide fixing enzymes within the reductive TCA cycle ; Pyruvate : ferredoxin oxidoreductase and 2-Oxoglutarate : ferredoxin oxidoreductase. As for Pyruvate : ferredoxin oxidoreductase, gene analysis was performed. Also, we constructed the gene disruption system in Hydrogenobacter thermophilus. By using the system, each one gene of two 2-Oxoglutarate : ferredoxin oxidoreductases was destroyed and the role of each gene product was clarified. In addition, ferredoxin works as an electron donor for Pyruvate : ferredoxin oxidoreductase and 2-Oxoglutarate : ferredoxin oxidoreductase reactions. Therefore, redox metabolism of ferredoxin(s) within Hydrogenobacter thermophilus was analyzed and total metabolism was grasped. Moreover, ATP : citrate reaction was clarified biochemically.
We also attempted heterologous expression of Acetyl-CoA carboxylase, which is a key carbon dioxide fixing enzyme within the 3-hydroxypropionate cycle. But, in spite of all the efforts, it has not been successful.
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