• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of function and biosynthetic pathway of novel glycosphingolipid found in filamentous fungi and their application for finding of antibiotics

Research Project

Project/Area Number 16380063
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

YAMAMOTO Kenji  Kyoto University, Graduate School of Biostudies, Professor, 生命科学研究科, 教授 (70109049)

Co-Investigator(Kenkyū-buntansha) KATAYAMA Takane  Ishikawa Prefectural University, Research Institute for Bioresources and Biotechnology, Lecturer, 生物資源工学研究所, 講師 (70346104)
SUGITA Mutsumi  Shiga University, Faculty of Liberal Arts and Education, Professor, 教育学部, 教授 (20024937)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2005: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2004: ¥7,800,000 (Direct Cost: ¥7,800,000)
KeywordsGlycosphingolipids / Inositol Phosphate Ceramide / Arcemonium / Trichoderma viride / Pathogenic fungi / Aureobasidium A / Zygomycetes / Mucor hiemalis / Mucor属 / ガラクトシルセラミド / ガラクトース転移酵素 / 植物病原菌 / ホスホコリン / 防御応答性遺伝子 / Aureobacidin A
Research Abstract

Sphingolipids are important components of the eukaryotic plasma membrane. They play important roles in membrane permeability and fluidity, and act as second messengers in response to various stress factors. In this study, we attempted to elucidate the function of novel glycosphingolipids from filamentous fungi.
1.Previously, we elucidated the structures of novel glycosylinositol phosphoceramides found in Acremonium sp. isolated from soil. These zwitterionic glycosphingolipids(ZGL)had different structures from those of glycosylinositol phosphoceramides in other fungi. Their structures were characterized to be composed of mannose and glucosamine attached to inositol phosphate. Two of these novel ZGLs were found in Trichoderma viride which is known as a pathogen that infects rice plants. One of two ZGLs had an additional phospho-choline group bound to the terminal mannose of the oligosaccharide. Such ZGLs had higher rice cell death-inducing activity. We confirmed it by analysis of expressi … More on of phenylalanine ammonia lyase (PAL) gene.
2.We found for the first time that Zygomycetes species showed resistance to Aureobasidin A, an antifungal agent. This antifungal agent is known to inhibit inositol phosphorylceramide (IPC) synthase in fungal cells. We analyzed glycosphingolipids of various Zygomycetes and found a novel family of neutral glycosphingolipids. We isolated these neutral glycosphingolipids from Mucor hiemalis and completely determined their structures by various analytical methods. As the result, we found that Zygomycetes species did not have inositol-containing sphingolipids but contained novel neutral glycosphingolipids consisting of galactose residues and phytoceramide. We supposed that the lack of a synthetic pathway for inositol-containing sphingolipids in their cells might be the cause of the resistance of Zygomycetes to Aureobasidin A. Then we attempted to clone the gene of the enzyme related with the biosynthetic pathway of neutral glycosphingolipids containing galactooligosaccharides. However, we do not succeed to do it. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (8 results)

All 2005 2004

All Journal Article (8 results)

  • [Journal Article] 食用キノコのスフィンゴ脂質 II.エリンギPleurotus eryngiiの多分岐型ガラクトースを含有したマイコグリコリピドの構造2005

    • Author(s)
      糸乗 先, 他
    • Journal Title

      滋賀大学教育学部紀要 55

      Pages: 15-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Studies on Sphingolipids of Edible Fungi II.Structures of Mycoglycolipids containing a Polytomy Galactose from the King Oyster Mushroom, Pleurotus eryngii2005

    • Author(s)
      Itonori, S., et al.
    • Journal Title

      Proceedings of Faculty of Liberal Arts and Education, Shiga University 55

      Pages: 15-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural Elucidation of Novel Phosphocholine-containing Glycosylinositol-phosphoceramides in Filamentous Fungi and Their Induction of Cell Death of Cultured Rice Cells.2004

    • Author(s)
      Aoki, K., et al.
    • Journal Title

      Biochem. J. 378

      Pages: 461-472

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural Elucidation of Novel Phosphocholine-Containing Glycosylinositol-Phosphoceramides in Filamentous Fungi.2004

    • Author(s)
      Yamada-Hada, J., et al.
    • Journal Title

      Chem. Pharm. Bull. 52

      Pages: 473-476

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Newly Discovered Neutral Glycosphingolipids in Aureobasidin A-resistant Zygomycetes.2004

    • Author(s)
      Aoki, K., et al.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 32028-32034

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural Elucidation of Novel Phosphocholine-containing Glycosylinositol-phosphoceramides in Filamentous Fungi and Their Induction of Cell Death of Cultured Rice Cells.2004

    • Author(s)
      Aoki, K., et al.
    • Journal Title

      Biochem.J. 378

      Pages: 461-472

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Structural Elucidation of Novel Phosphocholine-Containing Glycosylinositol-Phosphoceramides in Filamentous Fungi.2004

    • Author(s)
      Yamada-Hada, J., et al.
    • Journal Title

      Chem.Pharm.Bull. 52

      Pages: 473-476

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Newly Discovered Neutral Glycosphingolipids in Aureobasidin A-resistant Zygomycetes.2004

    • Author(s)
      Aoki, K., et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 32028-32034

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi