| Project/Area Number |
16380090
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
IMAIZUMI Katsumi Kyushu University, Faculty of Agriculture Applied Biological Sciences, Professor, 大学院・農学研究院, 教授 (90037466)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Masao Kyushu University, Faculty of Agriculture Applied Biological Sciences, Associate Professor, 大学院・農学研究院, 助教授 (90294909)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,900,000 (Direct Cost: ¥15,900,000)
Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2004: ¥10,600,000 (Direct Cost: ¥10,600,000)
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| Keywords | cholesterol / linoleic-acid / MCP-I / isoprostanes / MCP-1 / イソプロスタン / n-3系多価不飽和脂肪酸 / 動脈硬化症 / アポEノックアウトマウス / CCR2 |
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| Research Abstract |
The following events such as serum cholesterol level, arterial inflammatory reaction and oxidative stress in vivo, are involved in the initiation and development of atherosclerosis. The present study was carried out to obtain information on designing atherosclerosis-preventive foods. First, apo E-deficient mice were fed a diet either containing 10% linoleic acid or saturated fatty acid. The extent of atherosclerosis in the aortic root was found to be less in the mice fed the former diet than those fed the latter diet when examined at 9 weeks later. The mice fed the linoleic acid-rich diet had lower serum cholesterol level than did those fed the saturated fat, although aortic MCP-I mRNA abundance and the urinary 2,3-dinor-5,6-dihydro-8-iso-PGF2α level which is a marker of oxidative stress in vivo were greater in the linoleic acid-fed mice. To confirm that the serum cholesterol level is one of the most important biomarkers of atherosclerosis, apo E-deficient mice were fed the linoleic acid-rich diet with or without containing 0.04% cholesterol for 9 weeks. The cholesterol supplementation resulted in an increase of serum cholesterol level, and accompanied with an arterial lesion to a greater extent in the cholesterol-supplemented mice than in the unsupplemented ones. Cholesterol supplementation, however, had the effect neither on the linoleic acid-induced MCP-I mRNA abundance nor urinary isoprostanes excretion. Taken from these results together, the serum cholesterol level is one of the most important factors which lead to initiation and development of atherosclerosis.
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