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Development of washing method that efficiently removes central nervous system tissue on surface of beef carcass

Research Project

Project/Area Number 16380178
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

SEKIKAWA Mitsuo  Obihiro University of Agriculture and Veterinary Medicine, Graduate School, Animal and Food Hygiene, Professor, 大学院・畜産学研究科, 教授 (30120489)

Co-Investigator(Kenkyū-buntansha) ISHIOROSHI M.  Rakuno Gakuen University, Professor, 酪農学部, 教授 (70221067)
FUKUSHIMA M.  Obihiro Univ. of Agr. & Vet.Med., Associate Professor, 畜産学部, 助教授 (20231558)
STOU T.  Obihiro Univ. of Agr. & Vet.Med., Associate Professor, 畜産学部, 助教授 (90142794)
KUSHIDA K.  Obihiro Univ. of Agr. & Vet.Med., Associate Professor, 畜産学部, 助教授 (50271747)
SHIMADA K.  Obihiro Univ. of Agr. & Vet.Med., Associate Professor, 畜産学部, 助教授 (80301969)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2005: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2004: ¥10,700,000 (Direct Cost: ¥10,700,000)
KeywordsBSE / beef / central nervous system tissue / GFAP / lactic acid / prion protein / carcass washing machine / nozzle / 脳脊髄組織 / 発酵乳酸 / 大腸菌
Research Abstract

This research aimed to develop an automated cleaning machine of dressed carcasses to wash the processing of meats effectively for safety and security of meat. We have studied the washing equipments and cleaning agents to develop a washing method with which the central nervous system tissue (CNS), which exists potentially on beef carcass surface, could be efficiently removed without affecting the beef quality. First we confirmed the existence of CNS mainly targeting the commercially available meat products, by using the ELISA method whose marker is the Glial Fibrillary Acidic Protein (GFAP). As a result, almost no positive reaction was recognized in the commercial products, but the contamination in carcass cervical region was slightly detected in pork, venison, and beef. Since GFAP exists abundantly in glial cell and is hardly found in the other tissues, it has been a good marker for the CNS. But it was proposed that we should find a more effective marker because it was reveled that GFA … More P is degraded/reduced if it is analyzed by the electrophoresis after its mix with minced meat. In a cleaning experiment at a laboratory level, we found that the organic acids, especially the lactic acid, were effective in the removal of the CNS on the surface of meat. But when used in high concentration, not only was the cleaning effect decreased, but also the color of meat was deteriorated. Therefore, we proposed the optimum concentration was 0.5-1.0%. The washing machine designed in this study made for trial purposes can spray the cleaning solution over the upper, middle, and lower parts independently and a louver structure was fixed to the wall surfaces to prevent the liquid from rebounding from those surfaces. Furthermore, it was arranged that the cleaning pressure could be changed and the cleaning liquid could be exchanged (cleaning liquid tanks are prepared for the upper, middle, and lower parts independently). Also, considering the reproducibility of the volume and the pressure of the cleaning liquid, we adopted a method where the carcass would be washed by being turned around. By the way, the beef carcass is expensive and the cleaning machine would become too big, therefore the prototype was made by assuming the use of pork carcass. Following conditions, nozzle 8002 (discharge rate of 1.4L/min), pressure of more than 0.98MPa, and distance of within 70 cm, the brain tissue was sufficiently removed by washing of less than 30 seconds. In addition, in an experiment with a reduced pressure, it was proved effective if the lower part is washing in the last stage and used the 1% lactic acid solution. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (14 results)

All 2006 2005 2004 2003

All Journal Article (13 results) Book (1 results)

  • [Journal Article] Adzuki resistant starch lowered serum cholesterol and hepatic HMG-CoA mRNA levels and increased hepatic LDL-receptor and cholesterol 7a-hydroxylase mRNA levels in rats fed a cholesterol diet2006

    • Author(s)
      Han K.H., Iijuka, H., Shimada, K., Sekikawa, M., Kuramochi, K., Ohba, K., Ruvini, L., Chiji, H., Fukushima, M.
    • Journal Title

      British Journal of Nutrition (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 肉資源としてのシカ利用と養鹿2005

    • Author(s)
      関川三男, 増子義孝
    • Journal Title

      畜産の研究 59・1

      Pages: 41-47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Fermentation of meat with koji and commercial enzymes, and property of its extract,2005

    • Author(s)
      Nguyen Hien Trang, Ken-Ichiro Shimada, Mitsuo Sekikawa, Tomotada Ono, Masayuki Mikami
    • Journal Title

      Journal of the Science of Food and Agriculture 85・11

      Pages: 1829-1837

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Amylomyces rouxii Strain CBS 438.75 Affects Cholesterol Metabolism in Cholesterol-Fed Rats2005

    • Author(s)
      Fukushima, M., Han, K.H., Taneichi, Y., Iijima, S., Shimada, K., Saitoh, K., Oda, Y., Chiji, H., Sekikawa, M.
    • Journal Title

      Journal of Nutritional Science and Vitaminology 51・6

      Pages: 453-459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Fermentation of meat with koji and commercial enzymes, and property of its extract2005

    • Author(s)
      Nguyen Hien Trang, Ken-ichiro Shimada, Mitsuo Sekikawa, Tomotada Ono, Masayuki Mikami
    • Journal Title

      Journal of the Science of Food and Agriculture 85(11)

      Pages: 1829-1837

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amylomyces rouxii Strain CBS 438.75 Affects Cholesterol Metabolism in Cholesterol-Fed Rats2005

    • Author(s)
      Fukushima, M., Han, K.H., Taneichi, Y., Iijima, S., Shimada, K., Saitoh K., Oda, Y., Chiji, H., Sekikawa, M.
    • Journal Title

      Journal of Nutritional Science and Vitaminology 51(6)

      Pages: 453-459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 肉資源としてのシカ利用と養鹿2005

    • Author(s)
      関川三男, 増子孝義
    • Journal Title

      畜産の研究 59・1

      Pages: 47-52

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 骨付き生ハムの製造とその微生物学的および理化学的性質2005

    • Author(s)
      三上正幸, 島田謙一郎, 関川三男
    • Journal Title

      日本畜産学会報 76・1

      Pages: 29-38

    • NAID

      10025838087

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Species and muscle differences in L-carnitine levels in skeletal muscles based on a new simple assay2004

    • Author(s)
      Shimada, K., Y.Sakuma, J.Wakamatsu, M.Fukushima, M.Sekikawa, K.Kuchida, M.Mikami
    • Journal Title

      Meat Science 68

      Pages: 357-362

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Color and its stability in vension from Cervus nippon yesoensis (Japanese Yeso Deer)2004

    • Author(s)
      Sekikawa, M., K.H.Ham, K.Shimada, M.Fukushima, T.Ishikawa, C.H.Lee, M.Mikami
    • Journal Title

      Korean Journal Food Science Animal Resources 23(4)

      Pages: 309-314

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Resistant starch fraction prepared from Kintoki bean affects gene expression of genes associated with cholesterol metabolism in rats2004

    • Author(s)
      K.Han, M.Sekikawa, K.Shimada, K.Sasaki, K.Ohba, M.Fukushima
    • Journal Title

      Exp.Biol.Med. 229

      Pages: 787-792

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Species and muscle differences in L-carnitine levels in skeletal muscles based on a new simple assay2004

    • Author(s)
      K.Shimada, Y.Sakuma, J.Wakamatsu, M.Fukushima, M.Sekikawa, K.Kuchida, M.Mikami
    • Journal Title

      Meat Science 68

      Pages: 357-362

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Color and its stability in venison from Cervus nippon yesoensis(Japanese Yeso Deer)2003

    • Author(s)
      Sekikawa, M., K.H.Ham, K.Shimada, M.Fukushima, T.Ishikawa, C.H.Lee, M.Mikami
    • Journal Title

      Korean J. Food Sci. Ani. Resour. 23・4

      Pages: 309-314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 養鹿マニュアル2005

    • Author(s)
      関川三男(編集委員および執筆)他
    • Total Pages
      71
    • Publisher
      京橋堂印刷
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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