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Basic study on the production of somatic nuclear transferred chicken.

Research Project

Project/Area Number 16380184
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionUniversity of Tsukuba

Principal Investigator

TAJIMA Atsushi  University of Tsukuba, Graduate School of Life and Environmental Sciences, Associate Professor, 大学院生命環境科学研究科, 助教授 (40207030)

Co-Investigator(Kenkyū-buntansha) NAITO Mitsuru  Development and Differentiation Group, National Institute of Agro-Bioresources, Team Leader, 発生分化研究グループ発生制御研究チーム, チーム長(研究職) (70355733)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2005: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2004: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsPrimordial Germ Cells / Nuclear transfer / chicken / ウズラ / 鳥類 / 生殖系列 / 電気融合 / 移住能力
Research Abstract

1. Production of nuclear transferred GGC.:
Attempts were made to produce nuclear transferred gonadal germ cells (GGCs) using karyoplast produced from fibroblast. Karyoplasts were produced by using centrifuging fibroblast at 10,000 g 1 hour in the presence of 0.1% cytochalasin B. Average diameter of the karyoplasts were 10% smaller tcompared with intact fibroblast. Six nuclear transferred GGCs were produced using karyoplasts by electrofusion.
2. Production of intra-Genus nuclear transferred GGCs.
Attempts were made to produce intra-genus nuclear transferred GGC using chicken and quail. After electrofusion, 0.5-1.2 % of the nuclear GGCs was produced.
3. Effects of soft X exposure on the migrating ability of primordial germ cells.
Effects of soft X exposure on the migrating ability of primordial germ cells were examined. Results showed that the PGCs retain their migration ability toward gonadal ridge after transfer even after soft exposure.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (18 results)

All 2006 2005 2004 Other

All Journal Article (18 results)

  • [Journal Article] Effects of soft X-ray irradiation on restriction of proliferation of primordial germ cells in early chicken embryos.2006

    • Author(s)
      H.Nakamichi, A.Sano, T.Harumi, Y.Matsubara, A.Tajima, M.Kosugiyama, M.Naito
    • Journal Title

      Journal of Poultry Science 43 (4)

      Pages: 394-400

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Production of somatic nuclear transferred gonadal germ cells (GGCs) using somatic karyoplast in domestic chicken.2006

    • Author(s)
      Satoru Araya, Yusaku Kohara, Yukio Kanai, Atsushi Tajima
    • Journal Title

      World's Poultry Science Journal, Supplement. 62

      Pages: 528-529

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Production of somatic nuclear transferred gonadal germ cells (ntGGCs) using cryopreserved or serum starved somatic karyoplast in domestic chicken2006

    • Author(s)
      Yusaku Kohara, Yukio Kanai, Atsushi Tajima
    • Journal Title

      World's Poultry Science Journal, Supplement. 62

      Pages: 541-542

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Production of inter-genus somatic nuclear transferred GGCs in avian2006

    • Author(s)
      Susumu Ishiguro, Yukio Kanai, Atsushi Tajima
    • Journal Title

      World's Poultry Science Journal, Supplement. 62

      Pages: 540-541

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Effects of soft X-ray irradiation on restriction of proliferation of primordial germ cells in early chicken embryos2006

    • Author(s)
      H.Nakamichi, A.Sano, T.Harumi, Y.Matsubara, A.Tajima, M.Kosugiyama, M.Naito
    • Journal Title

      Journal of Poultry Science 43(4)

      Pages: 394-400

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Production of somatic nuclear transferred gonadal germ cells (GGCs) using somatic. karyoplast in domestic chicken.2006

    • Author(s)
      Satoru Araya, Yusaku Kohara, Yukio Kanai, Atsushi Tajima
    • Journal Title

      World's Poultry Science Journal, Supplement. 62

      Pages: 528-520

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of soft X-ray irradiation on restriction of proliferation of primordial germ cells in early chicken embryos.2006

    • Author(s)
      H.Nakamichi, A.Sano, T.Harumi, Y.Matsubara, A.Tajima, M.Kosugiyama, M.Naito
    • Journal Title

      Journal of Poultry Science 43(4)

      Pages: 394-400

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transformation of skeletal muscle from fast to slow-twitch during acquisition of cold tolerance in the chick.2005

    • Author(s)
      M.Hirabayashi, D.Ijiri, Y.Kamei, A.Tajima, Y.Kanai
    • Journal Title

      Endocrionlogy 146

      Pages: 2005-2005

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Production of germline chimeras by transferring GGCs collected from 7 and 9 day old chick embryos2004

    • Author(s)
      Tajima A, Minematsu T, Ohara M
    • Journal Title

      Animal Science Journal 75

      Pages: 85-88

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of Ultraviolet Irradiation on the Migratory ability of Primordial Germ Cells in the Domestic Chicken2004

    • Author(s)
      Minematsu T, Kanai Y, Tajima A.
    • Journal Title

      Journal of Poultry Science 41

      Pages: 110-119

    • Related Report
      2004 Annual Research Report
  • [Journal Article] An attempt to produce nuclear transferred PGCs using electrofusion in domestic chicken2004

    • Author(s)
      Minematsu T, Tajima A, Kanai Y.
    • Journal Title

      Animal Science Journal 75

      Pages: 271-274

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Simplified DNA Extraction Methods for Sexing Chick Embryos2004

    • Author(s)
      Minematsu T, Sugiyama M, Tohma Y, Kanai Y, Tajima A.
    • Journal Title

      Journal of Poultry Science 41

      Pages: 147-154

    • NAID

      130004446157

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The Migratory Ability of Gonadal Germ Cells in the Domestic Chicken2004

    • Author(s)
      Minematsu T, Kanai Y, Tajima A.
    • Journal Title

      Journal of Poultry Science 41

      Pages: 178-185

    • NAID

      10013357546

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of soft X-ray irradiation on the migratory ability of primordial germ cells in chickens

    • Author(s)
      M.Naito, T.Harumi, Y.Matsubara, A.Tajima, T.Kuwana
    • Journal Title

      British Poultry Science (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of soft X-ray irradiation on the migratory ability of primordial germ cells in chickens

    • Author(s)
      M.NAITO, T.HARUMI, Y.MATSUBARA, A.TAJIMA, T.KUWANA
    • Journal Title

      British Poultry Science (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of soft X-ray irradiation on the migratory ability of primordial germ cells in chickens

    • Author(s)
      M.Naito, T.Harumi, Y.Matsubara, A.Tajima, T.Kuwana
    • Journal Title

      British Poultry Science (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of soft X-ray irradiation on restriction of proliferation of primordial germ cells in early chicken embryos.

    • Author(s)
      H.Nakamichi, A.Sano, T.Harumi, Y.Matsubara, A.Tajima, M.Kosugiyama, M.Naito
    • Journal Title

      British Poultry Science (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of soft X-ray irradiation on the migratory ability of primordial germ cells in chickens

    • Author(s)
      M.NAITO, T.HARUMI, Y.MATSUBARA, A.TAJIMA, T.KUWANA
    • Journal Title

      British Poultry Science (in press)

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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