| Project/Area Number |
16380184
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
TAJIMA Atsushi University of Tsukuba, Graduate School of Life and Environmental Sciences, Associate Professor, 大学院生命環境科学研究科, 助教授 (40207030)
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| Co-Investigator(Kenkyū-buntansha) |
NAITO Mitsuru Development and Differentiation Group, National Institute of Agro-Bioresources, Team Leader, 発生分化研究グループ発生制御研究チーム, チーム長(研究職) (70355733)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2005: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2004: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | Primordial Germ Cells / Nuclear transfer / chicken / ウズラ / 鳥類 / 生殖系列 / 電気融合 / 移住能力 |
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| Research Abstract |
1. Production of nuclear transferred GGC.:
Attempts were made to produce nuclear transferred gonadal germ cells (GGCs) using karyoplast produced from fibroblast. Karyoplasts were produced by using centrifuging fibroblast at 10,000 g 1 hour in the presence of 0.1% cytochalasin B. Average diameter of the karyoplasts were 10% smaller tcompared with intact fibroblast. Six nuclear transferred GGCs were produced using karyoplasts by electrofusion.
2. Production of intra-Genus nuclear transferred GGCs.
Attempts were made to produce intra-genus nuclear transferred GGC using chicken and quail. After electrofusion, 0.5-1.2 % of the nuclear GGCs was produced.
3. Effects of soft X exposure on the migrating ability of primordial germ cells.
Effects of soft X exposure on the migrating ability of primordial germ cells were examined. Results showed that the PGCs retain their migration ability toward gonadal ridge after transfer even after soft exposure.
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