| Project/Area Number |
16380208
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Kitasato University |
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Principal Investigator |
TAKAI Shinji KITASATO UNIVERSITY, SCHOOL OF VETERINARY MEDICINE AND ANIMAL SCIENCES, PROFESSOR, 獣医畜産学部, 教授 (80137900)
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| Co-Investigator(Kenkyū-buntansha) |
KAKUDA Tsutomu KITASATO UNIVERSITY, SCHOOL OF VETERINARY MEDICINE AND ANIMAL SCIENCES, LECTURER, 獣医畜産学部, 講師 (80317057)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Rhodococcus equi / plasmid / Mongol / transmission / virulence / horse / infection / origin / ロドコッカス・エクイ / Pathogenicity island / 多様性 / 進化 / 中等度毒力株 / 遺伝子 / 病原性 / プラスミド / pathogenicity island / 毒力 |
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| Research Abstract |
At least three virulence levels of Rhodococcus equi have been identified : virulence, intermediate virulence, and avirulence. Virulent R.equi is characterized by the presence of virulence-associated 15- to 17-kDa antigens (VapA), virulence plasmid DNA of 85 to 90 kb, and suppurative pneumonia in foals (mouse LD_<50>=10^6). R. equi strains of intermediate virulence are identified by a virulence associated 20-kDa antigen (VapB) and virulence plasmid DNA of 79 to 100 kb and are found in the submaxillary lymph nodes of pigs (mouse LD_<50>=10^7). To analyze the difference of virulence levels in R.equi, the virulence plasmid of the R.equi strain A3 of intermediate virulence (pREA3) was sequenced and compared with the DNA sequence of the virulence plasmid (p33701). pREA3 was 79,288 bp and the plasmid encoded 71 open reading frames (ORFs). Of the 71 ORFs, 52 were found to be almost identical to those in the virulence plasmid (p33701). pREA3 also has a pathogenicity island containing 5 new viru
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lence-associated protein (vap) genes [vapI, vapJ (2 copies), vapK, and vapL] located upstream of vapB. There was extensive homology in the region except the pathogenicity island between p33701 and pREA3. These results suggest that the difference in the structure and function of the pathogenicity islands between p33701 and pREA3 might be responsible for the difference in virulence levels of R.equi. To elucidate the horizontal transfer of virulence genes encoded on the pathogenicity island, we compared the genomic physical maps of cryptic plasmids in a virulent R.equi with those in virulent and intermediately virulent R.equi. The similarities of the genomic physical maps of the cryptic plasmids with those of the virulence plasmids were observed except the sequence of the pathogenicity islands. Then, DNA sequence of the ORFs of the cryptic plasmids were analyzed and were found to be similar to the corresponding genes in the virulence plasmids. These results suggest that the horizontal transfer of PAI-encoded virulence determinants in. R.equi and has implications for genome evolution and diversity. The next step might be the investigation of the origin of the pathogenicity island. Less
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