An analysis of multiple learning mechanisms and its hierarchical regulation.
| Project/Area Number |
16390015
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
KAWAHARA Shigenori The University of Tokyo, Graduate School of Pharmaceutical Sciences, Associate Professor, 大学院薬学系研究科, 助教授 (10204752)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2004: ¥7,000,000 (Direct Cost: ¥7,000,000)
|
|---|
| Keywords | eyeblink conditioning / cerebellum / hippocampus / learning / memory |
|---|
| Research Abstract |
We investigated the roles of the cerebellum, the hippocampus and the medial prefrontal cortex in the eyeblink classical conditioning.
1. Cerebellar dependency in GluRδ2 KO mice.
We previously found that GluRδ2 KO mice, which have severe deficits in the cerebellar cortex, learn the short-trace eyeblink conditioning hippocampus-dependently. In this study, we investigated the dependency on the cerebellum. After learning the short-trace paradigm, GluRδ2 KO mice received bilateral cerebellar aspiration, two weeks of recovery and then re-conditioning. They showed a significant impairment on the first day, but re-learned during several days of re-conditioning. These results suggested that the mutant mice learn using the cerebellum, despite their deficits in the cerebellar cortex, but could also learn without the cerebellum maybe using another compensatory mechanism.
2. Cerebellar dependency in wild-type mice.
The cerebellar dependency was also examined in wild-type C57BL6 mice using the delay par
… More
adigm and the hippocampus-dependent long-trace paradigm. Wild-type mice also showed a severe impairment in memory retention and re-learning.
3. Hippocampal theta rhythm in GluRδ2 KO mice.
The theta power of the hippocampal EEG of GluRδ2 KO mice was significantly lower than that of wild-type mice during adaptation sessions. During conditioning sessions, the hippocampal theta power decreased in wild-type mice, but not in GluRδ2 KO mice. These results suggested that the hippocampal function might be altered in GluRδ2 KO mice due to their deficits of the cerebellar function.
4. Role of the medial prefrontal cortex in long-trace eveblink conditioning in rats.
The NMDA receptor antagonist APV, which was infused locally to the medial prefrontal cortex before or after the daily conditioning, severely impaired the learning in long-trace eyeblink conditioning in rats. This result suggested that NMDA receptors in the medial prefrontal cortex play important roles during memory acquisition as well as the memory consolidation. Less
|
Report
(4 results)
Research Products
(23 results)
