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Discovery of targets for treatment with renal cell carcinoma by gene and protein expression profile analysis

Research Project

Project/Area Number 16390040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionKobe University

Principal Investigator

OKUMURA Katsuhiko  Kobe University, Kobe University Hospital, School of Medicine, Professor, 医学部附属病院, 教授 (60025707)

Co-Investigator(Kenkyū-buntansha) SAKAEDA Toshiyuki  Kobe University, Kobe University Hospital, School of Medicine, Associate Professor, 医学部附属病院, 助教授 (00304098)
GOTOH Akinobu  Hyogo University, School of Medicine, Professor, 医学部, 教授 (70283885)
SHIRAKAWA Toshiro  Kobe University, School of Medicine, Associate Professor, 医学部, 助教授 (70335446)
OKAMURA Noboru  Kobe University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (60379401)
NAKAMURA Tsutomu  Kobe University, Kobe University Hospital, School of Medicine, Pharmacist, 医学部附属病院, 薬剤師 (80379411)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2005: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2004: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsProteome analysis / Transcriptome analysis / Renal cell carcinoma / Sorcin / VEGF / MALDI-TOF / Ms / MDR1 / sorcin / EGFR / 遺伝子型
Research Abstract

Renal cell carcinoma (RCC) is one of the most chemotherapy or radiotherapy-resistant malignant tumors, and therefore, the standard therapy is surgical treatment. Also, there is no efficient biomarker for diagnosis. To elucidate resistant mechanisms and discovery novel targets for treatment and biomarker for diagnosis, transcriptome and proteome analysis were carried out using RCC and adjacent normal tissues.
Since lower expression of sorcin in RCC was found, we investigated its role in cancer proliferation by RNA interference. Up-regulation of vascular endothelial growth factor (VEGF), a potent angiogenesis factor contributing to cancer proliferation, was found by knock-down of sorcin using siRNA in renal cell carcinoma cells, Caki-1. This suggested that lower expression of sorcin caused up-regulation of VEGF, thereby lead cancer proliferation.
To comprehensive analysis of protein expressions in RCC and adjacent normal tissues, samples were labeled with 2-nitrobeazenesulfonyl chloride with six ^<12>C or ^<13>C atoms. Then, they were applied MALDI-TOF/Ms and pair peak of 6 Da difference were analyzed and identified. Unknown proteins were identified in addition to known proteins, which have been reported to be up-regulated in RCC.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (9 results)

All 2006 2005 2004 Other

All Journal Article (9 results)

  • [Journal Article] MDR1 C3435T Polymorphism is predictive of later onset of ulcerative colitis in Japanese2006

    • Author(s)
      Osuga T
    • Journal Title

      Biological and Pharmaceutical Bulletin 第29巻・第2号

      Pages: 324-329

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] MDR1 C3435T Polymorphism is predictive of later onset of ulcerative colitis in Japanese.2006

    • Author(s)
      Osuga T
    • Journal Title

      Biological and Pharmaceutical Bulletin 29-2

      Pages: 324-329

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] EGFR mRNA is upregulated, but somatic mutaions of the gene are hardly found in renal cell carcinoma in Japanese patients2005

    • Author(s)
      Sakaeda T
    • Journal Title

      Pharmaceutical Reseach 第22巻・第10号

      Pages: 1757-1761

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] EGFR mRNA is upregulated, but somatic mutations of the gene are hardly found in renal cell carcinoma in Japanese patients.2005

    • Author(s)
      Sakaeda T
    • Journal Title

      Pharmaceutical Research 22-10

      Pages: 1757-1761

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] EGFR mRNA is upregulated, but somatic mutaions of the gene are hardly found in renal cell carcinoma in Japanese patients2005

    • Author(s)
      Sakaeda T
    • Journal Title

      Pharmaceutical Research 第22巻・第10号

      Pages: 1757-1761

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Carvedilol : a new candidate for reversal of MDR1/P-glycoprotein-mediated multidrug resistance.2004

    • Author(s)
      Takara K
    • Journal Title

      Anticancer Drugs 第15巻・第4号

      Pages: 303-309

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Antiproliferative activity of Rhinacanthus nasutus (L.) Kurz extracts and the active moiety, Rhinacanthin C.2004

    • Author(s)
      Gotoh A
    • Journal Title

      Biological Pharmaceutical Bulletin 第27巻・第7号

      Pages: 1070-1074

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Association of VEGF genotype with mRNA level in colorectal adenocarcinomas.2004

    • Author(s)
      Yamamori M
    • Journal Title

      Biochemical Biophysical Research Communication 第325巻・第1号

      Pages: 144-150

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of micafungin on CYP3A4 and MDR1 activities and its comparison with azole antifungal drugs.

    • Author(s)
      Sakaeda T
    • Journal Title

      Journal of Pharmacy and Pharmacology (印刷中)(未定)

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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