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Study on switching mechanisms of protein-protein interactions underlying the establishment of cell polarity

Research Project

Project/Area Number 16390073
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

HATA Yutaka  Tokyo Medical and Dental University, Graduate School of Medicine, Professor, 大学院・医歯学総合研究科, 教授 (80313237)

Co-Investigator(Kenkyū-buntansha) HIRABAYASHI Susumu  Tokyo Medical and Dental University, Graduate School of Medicine, Assistant Professor, 大学院・医歯学総合研究科, 助手 (80376730)
IIDA Junko  Tokyo Medical and Dental University, Graduate School of Medicine, Assistant Professor, 大学院・医歯学総合研究科, 助手 (80376805)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2005: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2004: ¥9,300,000 (Direct Cost: ¥9,300,000)
Keywordsepithelial cell junction / neural synapse / cell adhesion / cell polarity / 細胞骨格 / シグナル伝達
Research Abstract

Multicellular organisms develop functional domains such as epithelial cell junctions, neural synapses and lipid rafts on plasma membranes, which are important for the efficient intercellular signal transductions. These domains are composed of distinct protein components that interact with each other in manifold ways. Scaffold proteins have emerged as key molecules underlying basal molecular architectures of cell junctions. Scaffold proteins have multiple modules that bind a wide variety of proteins and facilitate the interactions among these ligands. As more than one ligands bind to the same module, some components can interact with a scaffold protein simultaneously, whereas others can not. Thereby, the combination of proteins assembled by a scaffold protein should alter depending on the cellular context. We currently examine which proteins are assembled by scaffold proteins at various stages of the maturation of cell junctions.
1) We have reported that synaptic scaffold molecule (S-SCAM) is involved in the accumulation of synaptic cell adhesion molecule named neuroligin. S-SCAM appears to provide a scaffold to activate a small GTP-binding protein implicated in the regulation of the cytoskeleton in neural dendritic spines.
2) Membrane-associated guanylate kinase with inverted organization (MAGI)-1 is an epithelial isoform of S-SCAM. We have recently revealed in the immunoelectron microscope the localization of MAGI-1 at slit diaphragm in kidney. MAGI-1 interacts with nephrin, a cell adhesion molecule that is an essential component of slit diaphragm.
3) Junctional adhesion molecule (JAM) 4 was identified as a ligand for MAGI-1. We have identified Ligand-of-Numb X (LNX) 1 as a novel JAM4-binding partner and analyzed the effect of LNX1 on endocytosis of JAM4.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (22 results)

All 2005 2004 Other

All Journal Article (17 results) Book (5 results)

  • [Journal Article] MAGI-1 is a component of the glomerular slit diaphragm that is tightly associated with nephrin.2005

    • Author(s)
      Hirabayashi S et al.
    • Journal Title

      Lav. Invest. 85巻12号

      Pages: 1528-1543

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] MAGI-1 is a component of the glomerular slit diaphragm that is tightly associated with nephrin.2005

    • Author(s)
      Hirabayashi S. et al.
    • Journal Title

      Lav.Invest. 85

      Pages: 1528-1543

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] MAGI-1 is a component of the glomerular slit diaphragm that is tightly associated with nephrin.2005

    • Author(s)
      Hirabayashi S et al.
    • Journal Title

      Lab.Invest. 85巻12号

      Pages: 1528-1543

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Synaptic scaffolding molecule is involved in the synaptic clustering of neuroligin.2004

    • Author(s)
      Iida J et al.
    • Journal Title

      Mol. Cell. Neurosci. 27巻4号

      Pages: 497-508

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] JAM4 enhances hepatocyte growth factor-mediated branching and scattering of Madin-Darby canine kidney cells.2004

    • Author(s)
      Mori H et al.
    • Journal Title

      Genes Cells 9巻9号

      Pages: 811-819

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synaptic scaffolding molecule interacts with Axin.2004

    • Author(s)
      Hirabayashi S et al.
    • Journal Title

      J. Neurochem. 90巻2号

      Pages: 332-339

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synaptic scaffolding molecule is involved in the synaptic clustering of neuroligin.2004

    • Author(s)
      Iida J.et al.
    • Journal Title

      Mol.Cell.Neurosci. 27

      Pages: 497-508

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] JAM4 enhances hepatocyte growth factor-mediated branching and scattering of Madin-Darby canine kidney cells.2004

    • Author(s)
      Mori H.et al.
    • Journal Title

      Genes Cells 9

      Pages: 811-819

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synaptic scaffolding molecule interacts with Axin.2004

    • Author(s)
      Hirabayashi S. et al.
    • Journal Title

      J.Neurochem. 90

      Pages: 332-339

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synaptic scaffolding molecule is involved in the synaptic clustering of neuroligin2004

    • Author(s)
      Iida, J. et al.
    • Journal Title

      Mol.Cell.Neurosci. 27巻4号

      Pages: 497-508

    • Related Report
      2004 Annual Research Report
  • [Journal Article] JAM4 enhances hepatocyte growth factor-mediated brandching and scattering of Madin-Darby canine kidney cells2004

    • Author(s)
      Mori, H. et al.
    • Journal Title

      Genes Cells 9巻9号

      Pages: 811-819

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synaptic scaffolding molecule interacts with Axin2004

    • Author(s)
      Hirabayashi, S. et al.
    • Journal Title

      J.Neurochem. 90巻2号

      Pages: 332-339

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ligand-of-Numb X is an endocytic scaffold for junctional adhesion molecule 4.

    • Author(s)
      Kansaku A et al.
    • Journal Title

      Oncogene (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Receptor for advanced glycation end-products is a marker of type I cell injury in acute lung injury

    • Author(s)
      Uchida T et al.
    • Journal Title

      Am. J. Res. Crit. Care Med. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ligand-of-Numb X is an endocytic scaffold for junctional adhesion molecule 4

    • Author(s)
      Kansaku A. et al.
    • Journal Title

      Oncogene (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Receptor for advanced glycation end-products is a marker of type I cell injury in acute lung injury.

    • Author(s)
      Uchida T. et al.
    • Journal Title

      Am.J.Res.Crit.Care Med. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ligand-of-Numb X is an endocytic scaffold for junctional adhesion molecule 4.

    • Author(s)
      Kanasaku A et al.
    • Journal Title

      Oncogene (In press)

    • Related Report
      2005 Annual Research Report
  • [Book] 遺伝子制御による選択的シナプス強化・除去2005

    • Author(s)
      畑 裕
    • Total Pages
      202
    • Publisher
      クバプロ
    • Related Report
      2005 Annual Research Report
  • [Book] Tight junction2005

    • Author(s)
      Hirabayashi, S., Hata, Y.
    • Publisher
      Landes Bioscience (In press)
    • Related Report
      2004 Annual Research Report
  • [Book] Encyclopedic Reference of Neuroscience

    • Author(s)
      Hata Y, Iida J
    • Publisher
      Springer(In press)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Tight junction

    • Author(s)
      Hirabayashi S, Hata Y
    • Publisher
      Landes Bioscience(In press)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Encyclopedic Reference of Neuroscience

    • Author(s)
      Hata Y, Iida J
    • Publisher
      Springer (In press)
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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