• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Chromatin replication through cellular proliferation in eukaryotic cells

Research Project

Project/Area Number 16390085
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionNational Institute of Genetics

Principal Investigator

SHIBAHARA Kei-ichi  National Institute of Genetics, Dep. of Integrated Genetics, Associate Prof., 総合遺伝研究系, 助教授 (20263098)

Co-Investigator(Kenkyū-buntansha) NISHIJIMA Hitoshi  National Institute of Genetics, Assistant Prof., 助手 (60425410)
OGAWA Yuya  National Institute of Genetics, Assistant Prof., 助手 (10370123)
ONO Tatsuya  National Institute of Genetics, Post Doc., 研究員 (50419850)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2006: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2005: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2004: ¥6,000,000 (Direct Cost: ¥6,000,000)
Keywordschromatin / CAF-1 / nucleosome / DNA replication / chromatin replication / epigenetics / DNA複製 / クロマチン複製 / ヌクレオソーム / ヌクレオソームアセンブリー / ヒストン / ジーンターゲティング / 細胞記憶 / DDM1 / モノユビキチン化
Research Abstract

A newly replicated DNA is assembled into nucleosome soon after the passage of replication fork. This rapid formation of nucleosome is functionally linked to DNA replication and plays a critical role for the maintenance of genome integrity in proliferating cells (Shibahara and Stillman, Cell, 1999). Two histone-binding proteins, CAF-1 and ASF1, are assumed to be involved in some process of this replication-coupled nucleosome assembly reaction.
Using CAF-1-deficient chicken DT40 cell lines, we had recently shown that without CAF-1 and/or ASF1 function, S-phase progression was delayed and a rapid nucleosome assembly during DNA replication was disturbed in vivo. (Sanematsu, et al., J.Biol.Chem., 2006). This is the first direct evidence for the involvement of CAF-1 and ASF1 in a rapid nucleosome assembly during DNA replication in vivo. In addition, we obtained some interesting evidence for that CAF-1 is involved in Chk1-dependent checkpoint pathway after the treatment with HU (Takami et al., … More Mol.Biol.Cell, 2007).
The loss-of-function mutants of caf-1 (fas) in Arabidopsis displayed severely disturbed cellular and functional organization of both meristems (Kaya et al., Cell, 2001). We also had shown that transcriptional gene silencing (TGS) of endogenous CACTA transposons was released infrequently in a stochastic manner in fas mutants without decreasing DNA methylation. Other endogenous silent genes at different chromosomal sites were also transcriptionally activated non-concomitantly with each other. Furthermore, TGS of the silent transgene β-glucuronidase (GUS) was also de-repressed randomly in fas mutants, irrespectively of developmental abnormalities, and the activated state of GUS was maintained during growth to produce clusters of cells expressing GUS (Ono et al., Genes Cells, 2006). Taken together, we strongly suggest that CAF-1 ensures stable maintenance of epigenetic states through multiple rounds of cell divisions and that defects in CAF-1 functions explain the stochastic occurrence of various phenotypes in the fas mutants. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (38 results)

All 2007 2006 2005 2004

All Journal Article (30 results) Book (2 results) Patent(Industrial Property Rights) (6 results)

  • [Journal Article] Chromatin assembly factor-1 is required for rapid nucleosome formation during DNA replication and efficient Chk1 activation in vertebrate chicken DT40 cells.2007

    • Author(s)
      Takami, Y.
    • Journal Title

      Mol. Biol. Cell. 18(1)

      Pages: 129-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chromatin assembly factor-1 is required for rapid nucleosome formation during DNA replication and efficient Chk1 activation in vertebrate chicken DT40 cells.2007

    • Author(s)
      Takami, Y.
    • Journal Title

      Mol.Biol.Cell 18

      Pages: 129-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chromatin assembly is required for rapid nucleosome formation during DNA replication and efficient Chk1 activation in vertebrate chicken DT40 cells.2007

    • Author(s)
      Takami, Y., Ono, T., Fukagawa, T., K-i, Shibahara, T.Nakayama.
    • Journal Title

      Mol. Biol. Cell. 18(1)

      Pages: 129-141

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis2006

    • Author(s)
      Ono, T.
    • Journal Title

      Genes Cells (Cover to this issue) 18

      Pages: 153-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe.2006

    • Author(s)
      Nishijima, H.
    • Journal Title

      Mol. Biol. Cell 17

      Pages: 2524-2536

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone acetyltransferase I is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.2006

    • Author(s)
      Barman, HK.
    • Journal Title

      Biochem Biophys Res Commun 345

      Pages: 1547-1557

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Simple, one-week method to construct gene-targeting vectors : application to production of human knockout cell lines2006

    • Author(s)
      Iizzumi, S.
    • Journal Title

      BioTechniques 41(3)

      Pages: 311-316

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhanced homologous recombination and T-DNA integration in chromatin assembly factor-I mutants in Arabidopsis thaliana.2006

    • Author(s)
      Endo, M.
    • Journal Title

      EMBO J. 25(23)

      Pages: 5579-5590

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis.2006

    • Author(s)
      Ono, T.
    • Journal Title

      Genes Cells (Cover to this issue) 18

      Pages: 153-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe.2006

    • Author(s)
      Nishijima, H.
    • Journal Title

      Mol.Biol.Cell 17

      Pages: 2524-2536

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Asfl is required for viability and chromatin assembly during DNA replication in vertebrate cells.2006

    • Author(s)
      Sanematsu, F.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 13817-13827

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.2006

    • Author(s)
      Barman, HK.
    • Journal Title

      Biochem Biophys Res Commun 345

      Pages: 1547-1557

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Simple, one-week method to construct gene-targeting vectors : application to production of human knockout cell lines.2006

    • Author(s)
      Iizzumi, S.
    • Journal Title

      BioTechniques 41(3)

      Pages: 311-316

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhanced homologous recombination and T-DNA integration in chromatin assembly factor-1 mutants in Arabidopsis thaliana.2006

    • Author(s)
      Endo, M.
    • Journal Title

      EMBO J. 25(23)

      Pages: 5579-5590

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis.2006

    • Author(s)
      Ono, T., Kaya, H., Takeda, S., Abe, Y., Ogawa, Y., Kato, M., Kakutani, T., Scheid, OM., Araki, T., Shibahara, K-i
    • Journal Title

      Genes Cells (Covert to this issue) 18

      Pages: 153-162

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe.2006

    • Author(s)
      Nishijima, H., Nakayama, JI., Yoshioka, T., Kusano, A., Nishitani, H., Shibahara, K-i., Nishimoto, T.
    • Journal Title

      Mol. Biol. Cell 17

      Pages: 2524-2536

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Asfl is required for viability and chromatin assembly during DNA replication in vertebrate cells.2006

    • Author(s)
      Sanematsu, F., Takami, Y., Barman, HK., Fukagawa, T., Ono, T., Shibahara, K-i., Nakayama T.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 13817-13827

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Histone acetyltransfcrase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.2006

    • Author(s)
      Barman, HK., Takami Y., Ono T., Nishijima, H., Sanematsu, F., Shibahara, K-i., Nakayama, T.
    • Journal Title

      Biochem Biophys Res Commun 345

      Pages: 1547-1557

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Simple, one-week method to construct gene-targeting vectors : application to production of human knockout cell lines.2006

    • Author(s)
      Iizzumi., S, Nomura, Y., So, S., Uegaki, K., Aoki, K., Shibahara K-i., Adachi, N., Koyama H.
    • Journal Title

      Bio Techniques 41(3)

      Pages: 311-316

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhanced homologous recombination and T-DNA integration in chromatin assemble factor-1 mutants in Arabidopsis thaliana.2006

    • Author(s)
      Endo, M, Ishikawa, Y, Kaya, H, Araki, T, Shibahara, K-i, Ito, Y, Osakabe, K, Abe, K, Ichikawa, H, Valentine, L, Hohn, B, Toki, S.
    • Journal Title

      EMBO J. 25(23)

      Pages: 5579-5590

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells.2006

    • Author(s)
      Sanematsu, F.
    • Journal Title

      J.Bio.Chem. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe.2006

    • Author(s)
      Nishijima, H.
    • Journal Title

      Mol.Biol.Cell. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis2006

    • Author(s)
      Ono, T.
    • Journal Title

      Genes to Cells 18

      Pages: 153-162

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Histone variant macroH2A1.2 is mono-ubiquitinated at its histone domain.2005

    • Author(s)
      Ogawa, Y.
    • Journal Title

      Biochem Biophys Res Commun 336

      Pages: 204-209

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Asfl is required for viability and chromatin assembly during DNA replication in vertebrate cells.2005

    • Author(s)
      Sanematsu, F.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 13817-13827

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone variant macroH2A1.2 is mono-ubiquitinated at its Histone domain.2005

    • Author(s)
      Ogawa, Y.
    • Journal Title

      Biochem Biophys Res Commun 336

      Pages: 204-209

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone variant macroH2A1.2 is mono-ubiquitinated at its histone domain.2005

    • Author(s)
      Ogawa, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 336

      Pages: 204-209

    • Related Report
      2005 Annual Research Report
  • [Journal Article] BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.2004

    • Author(s)
      Takeda, S.
    • Journal Title

      Genes Dev 18

      Pages: 782-793

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] BRU1, a novel link between responses to DNA damage and Epigenetic gene silencing in Arabidopsis.2004

    • Author(s)
      Takeda, S.
    • Journal Title

      Genes Dev 18

      Pages: 782-793

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.2004

    • Author(s)
      Takeda, S., et al.
    • Journal Title

      Genes & Development 18

      Pages: 782-793

    • Related Report
      2004 Annual Research Report
  • [Book] DNA複製・DNA修復がわかる「DNA修復とヌクレオチド除去修復2004

    • Author(s)
      久保知大, 柴原慶一
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] わかる実験医学シリーズ「DNA複製・修復がわかる」2004

    • Author(s)
      久保 知大 他
    • Total Pages
      5
    • Publisher
      羊土社
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 標的組換え細胞とランダム組換え細胞を判別する方法およびその用途2007

    • Inventor(s)
      柴原慶一, 西嶋仁, 国田嘉元
    • Industrial Property Rights Holder
      情報・システム研究機構
    • Industrial Property Number
      2007-011971
    • Filing Date
      2007-04-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] テトラサイクリン誘導型の遺伝子発現系に使用する目的遺伝子導入ベクター、トランスアクチベーター発現用ベクターおよびその用途2006

    • Inventor(s)
      柴原慶一, 小野達也
    • Industrial Property Rights Holder
      情報・システム研究機構
    • Industrial Property Number
      2006-288020
    • Filing Date
      2006-10-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] デトラサイクリン誘導体の遺伝子発現系に使用する目的遺伝子導入ベクター、トランスアクチベーター発現用ベクターおよびその用途2006

    • Inventor(s)
      柴原慶一, 小野達也
    • Industrial Property Number
      2006-288020
    • Filing Date
      2006-10-23
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 標的組換え細胞とランダム組換え細胞を判別する方法およびその用途2006

    • Inventor(s)
      柴原慶一, 西嶋仁, 国田嘉元
    • Industrial Property Number
      2007-011971
    • Filing Date
      2006-04-27
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 遺伝子導入方法、ジーンターゲッティング方法及びトランスジェニック植物の製造方法2005

    • Inventor(s)
      柴原慶一
    • Industrial Property Rights Holder
      情報・システム研究機構
    • Industrial Property Number
      2005-006544
    • Filing Date
      2005-03-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 遺伝子導入方法、ジーンターゲッティング方法及びトランスジェニック植物の製造方法2005

    • Inventor(s)
      柴原 慶一
    • Industrial Property Rights Holder
      柴原 慶一
    • Industrial Property Number
      2005-065442
    • Filing Date
      2005-03-09
    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi