Molecular basis of Zic-mediated developmental control
| Project/Area Number |
16390086
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | RIKEN |
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Principal Investigator |
ARUGA Jun RIKEN BSI, Laboratory for Comparative.Neurogenesis, Laboratory Head, 比較神経発生研究チーム, チームリーダー (10232076)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Akira RIKEN BSI, Laboratory for Comparative.Neurogenesis, Research Scientist, 比較神経発生研究チーム, 研究員 (70373264)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2005: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | Zic / transcription factor / cell differentiation / neural development / gene targeting / transcriptional complex / protein modification / congenital anomaly / zincフィンガータンパク質 / タンパク質間相互作用 / 遺伝子発現制御 / 転写調節 / 動物発生 / 構造生物学 / zincフィンガー / 蛋白質間相互作用 / 遺伝子発現調節 / 細胞内局在 |
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| Research Abstract |
Zic genes are known for causal genes of holoprosencephaly, Dandy-Walker malformation, and heterotasxy.. In this study, following points of the Zic gene family have been dealt with. (1) Identification of novel Zic-binding proteins, (2) The biological significance of the protein-to-protein interaction between Zic and Zic-binding proteins, (3) Post-translational modification of Zic proteins, (4) Bio-signals that have contact points with Zic proteins.
Following points were revealed and publicized in the scientific community.
(1) Identification of novel two Zic2-containing high-molecular-weight molecular complexes (Ishiguro et al.,2007). Identification of I-mfa as a Zic-repressor by yeast two-hybrid screening. (2,3) Identification of a Zic2-kinase in the HMW molecular complexes. The phosphorylation of Zic2 protein was shown to be critical in the transcriptional regulatory processes. Other modifications sites in Zic proteins have been identified and characterized by mass spectrometry analysis. (4) The Zic related bio-signals was investigated by analyzing the Zic1/3 and Zic2/3 compound mutant mice (Inoue et al., 2007a; 2007b). In particular, we revealed that Zic1 and Zic3 have critical roles in the expansion of forebrain neuronal precursors. Wnt signals was suggested to be involved in the Zic mediated developmental control. In addition, functional similarity and difference among vertebrates Zic genes have been investigated in mouse and Xenopus (Ishiguro et al.,2004; Fujimi et al.,2006).
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Elucidation of penetrance variability of a ZIC3 mutation in a family with complex heart defects and functional analysis of ZIC3 mutations in the first zinc finger domain.2007
Author(s)
Chhin B, Hatayama M, Bozon D, Ogawa M, Schon P, Tohmonda T, Sassolas F, Aruga J, Valard AG, Chen SC, Bouvagnet P
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Journal Title
Hum Mutat 28
Pages: 563-570
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] A wide-range phylogenetic analysis of Zic proteins : implications for correlations between protein structure conservation and body plan complexity.2006
Author(s)
Aruga J, Kamiya A, Takahashi H, Fujimi TJ, Shimizu Y, Ohkawa K, Yazawa S, Umesono Y, Noguchi H, Shimizu T, Saitou N, Mikoshiba K, Sakaki Y, Agata K, Toyoda A
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Journal Title
Genomics 87
Pages: 783-792
Description
「研究成果報告書概要(欧文)」より
Related Report
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