Study of analysis for vascular damage due to embolism and inflammation

• Project/Area Number: 16390161
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Asahikawa Medical College
• Principal Investigator: WAKAMIYA Nobutaka Asahikawa Medical College, Faculty of Medicine, Professor, 医学部, 教授 (20210867)
• Co-Investigator(Kenkyū-buntansha): FUKUZAWA Jun Faculty of Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (40333695) ; SUZUKI Yasuhiko Hokkaido University, Research Center for Zoonosis Control, Professor, 人獣共通感染症リサーチセンター, 教授 (90206540) ; YOSHIDA Itsuro Asahikawa Medical College, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20041816) ; OGASAWARA Masahiro Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (40185492) ; OHTANI Katsuki Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (90396367)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥14,400,000 (Direct Cost: ¥14,400,000); Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
• Keywords: collectin / analysis / blood / 梗塞 / 炎症 / 定量的解析

Research Abstract

CL-P1 is a new collectin which was found by Ohtani at 2001. It was expressed in vascular endothelial cells in murine tissues and also found in vascular endothelial cell lines. Here we established the antibody against above CL-P1 to investigate their biological functions. We have immunized mice with recombinant CL-P1 protein and made hybridoma for monoclonal antibodies. Several monoclonal antibodies were established and were divided to two or three groups. We tried to make the analysis method to detect CL-P1 using above monoclonal antibodies. We made several prototype assay systems but we could not get a good result because of less CL-P1 volume. Now we began to make a new analysis system using polyclonal antibodies.

Research Products

• [Journal Article] Anti-influenza A virus activities of mannan-binding lectins and bovine conglutinin (2007)
  • Author(s): Kawai T, Kase T, Suzuki Y, Eda S, Sakamoto T, Ohtani K, Wakamiya N
  • Journal Title: J Vet Med Sci. 69(2) Pages: 221-224
  • NAID: 110006201244
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Anti-influenza A virus activities of mannan-binding lectins and bovine conglutinin. (2007)
  • Author(s): Kawai T, Kase T, Suzuki Y, Eda S, Sakamoto T, Ohtani K, Wakamiya N.
  • Journal Title: J Vet Med Sci. 69(2) Pages: 221-4
  • NAID: 110006201244
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anti-influenza A virus activities of mannan-binding lectins and bovine conglutinin (2007)
  • Author(s): Kawai T, Kase T, Suzuki Y, Eda S, Sakamoto T, Ohtani K, Wakamiya N.
  • Journal Title: J Vet Med Sci. 69(2) Pages: 221-224
  • NAID: 110006201244
• [Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47. II. Tne HSP47-binding structural motif in collagens and related proteins (2006)
  • Author(s): Koide T, Nishikawa Y, Asada S, Yamazaki CM, Takahara Y, Homma DL, Otaka A, Ohtani K, Wakamiya N, Nagata K, Kitagawa K
  • Journal Title: J Biol Chem. 281 Pages: 11177-11185
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and characterization of a novel human collectin CL-K1 (2006)
  • Author(s): Keshi H, Sakamoto T, Kawai T, Ohtani K, Katoh T, Jang S-J, Motomura W, Yoshizaki T, Fukuda M, Koyama S, Fukuzawa J, Fukuoh A, Yoshida I, Suzuki Y, Wakamiya N
  • Journal Title: Microbiol. Immunol. 50(12) Pages: 1001-1013
  • NAID: 10018639470
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] コレクチンとその免疫応答における役割 (2006)
  • Author(s): 大谷克城, 鈴木定彦, 若宮伸隆
  • Journal Title: 臨床免疫 43(3) Pages: 266-274
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 薬剤耐性菌と院内感染対策 (2006)
  • Author(s): 吉田逸朗, 張成宰, 若宮伸隆
  • Journal Title: 北海道医報 7 道医シリーズ第45編 感染症 第45編 Pages: 71-74
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and characterization of a novel human collectin CL-K1. (2006)
  • Author(s): Keshi H, Sakamoto T, Kawai T, Ohtani K, Katoh T, Jong S-J, Motomura W, Yoshizaki T, Fukuda M, Koyama S, Fukuzawa J, Fukuoh A, Yoshida I, Suzuki Y, Wakamiya N.
  • Journal Title: Microbiol.Immunol. 50(12) Pages: 1001-1013
  • NAID: 10018639470
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins. (2006)
  • Author(s): Koide T, Nishikawa Y, Asada S, Yamazaki CM, Takahara Y, Homma DL, Otaka A, Ohtani K, Wakamiya N, Nagata K, Kitagawa K.
  • Journal Title: J.Biol.Chem. 281(16) Pages: 11177-85
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47.II. The HSP47-binding structural motif in coll agens and related proteins (2006)
  • Author(s): Koide T, Nishikawa Y, Asada S, Yamazaki CM, Takahara Y, Homma DL, Otaka A, Ohtani K, Wakamiya N, Nagata K, Kitagawa K.
  • Journal Title: J Biol Chem. 281 Pages: 11177-11185
• [Journal Article] Lack of relationship between mannose- binding lectin variant alleles and risk of arterial thrombosis in Japanese patients with systemic lupus erythematosus. (2005)
  • Author(s): Takahashi R, Tsutsumi A, Ohtani K, Wakamiya N. Sumida T.
  • Journal Title: Mod.Rheumatol. 15 Pages: 459-460
  • NAID: 10016914822
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Kato, S. : Preparation of monoclonal antibodies cross- reactive with Orthopoxviruses and their application for direct immunofluorescence test. (2005)
  • Author(s): Kitamoto, N., Kobayashi, T., Kato, Y., Wakamiya, N, Ikuta, K., Tanaka, T., Ueda, S., Miyamoto, H.
  • Journal Title: Microbiol.Immunol. 49(3) Pages: 219-225
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Association of mannose-binding lectin (MBL) gene polymorphism and serum MBL concentration with characteristics and progression of systemic lupus erythematosus. (2005)
  • Author(s): Takahashi, R.Tsutsumi, A.Ohtani, K.Mukai, Y.Goto, D.Matsumoto, I.Wakamiya, N Sumida, T.
  • Journal Title: Ann.Rheum.Dis. 64 Pages: 311-314
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Association of mannose-binding lectin (MBL) gene polymorphism and serum MBI concentration with characteristics and progression of systemic lupus erythematosus. (2005)
  • Author(s): Takahashi, R., Tsutsumi, A., Ohtani, K., Mukai, Y., Goto, D., Matsumoto, I., Wakamiya, N, Sumida, T
  • Journal Title: Ann.Rheum.Dis. 64 Pages: 311-314
• [Journal Article] Possibility of rapid detection for Variola virus using monoclonal antibody cross-reacted with Orthopoxviruses. (2005)
  • Author(s): Kitamoto, N., Kobayashi, T., Kato, Y., Wakamiya, N., Ikuta, K., Tanaka, T., Miyamoto, H., Kato, S
  • Journal Title: Microbiol.Immunol 49 Pages: 219-225
• [Journal Article] Lack of relationship between mannosebinding lectin variant alleles and risk of arterial thrombosis in Japanese patients with systemic lupus erythematosus. (2005)
  • Author(s): Takahashi R, Tsutsumi A, Ohtani K, Wakamiya N, Sumida, T.
  • Journal Title: Mod.Rheumatol.. 15 Pages: 459-460
• [Journal Article] 補体系異常と感染 (2005)
  • Author(s): 大谷克城, 若宮伸隆
  • Journal Title: 臨床と微生物 32(3) Pages: 261-267
  • NAID: 10015687392
• [Journal Article] Association of mannose-binding lectin (MBL) gene polymorphism and serum MBL concentration with characteristics and progression of systemic lupus erythematosus (2005)
  • Author(s): Takahashi, R., Tsutsumi, A., Ohtani, K., Mukai, Y., Goto, D., Matsumoto, I., Wakamiya, N., Sumida, T.
  • Journal Title: Ann.Rheum.Dis. 64 Pages: 311-314
• [Journal Article] Anti- mannose binding lectin antibodies in sera of Japanese patients with systemic lupus erythematosus. (2004)
  • Author(s): Takahashi R, Tsutsumi A, Ohtani K, Goto D, Matsumoto I, Ito S, Wakamiya N, Sumida T.
  • Journal Title: Clin Exp Immunol. 136(3) Pages: 585-90
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anti-mannose binding lectin antibodies in sera of Japanese patients with systemic lupus erythematosus. (2004)
  • Author(s): Takahashi R, Tsutsumi A, Ohtani K, Goto D, Matsumoto I, Ito S, Wakamiya N, Sumida T.
  • Journal Title: Clin Exp Immunol. 136(3) Pages: 585-590
• [Journal Article] 生体防御レクチンとしてのコレクチンファミリー (2004)
  • Author(s): 若宮伸隆, 吉田逸朗, 小笠原正洋, 福澤純, 大谷克城, 小山聡
  • Journal Title: 北海道医学雑誌 79(1) Pages: 3-7
  • NAID: 110000339839
• [Journal Article] 自然免疫におけてMBLはどのような役割を果たすのか (2004)
  • Author(s): 若宮伸隆
  • Journal Title: 分子消化器病 1(2) Pages: 149-155
• [Journal Article] Collagen- related lectins in innate immunity, : The novel collectins, CL-L1,CL-P1,CL-K1..
  • Author(s): Suzuki, Y., Ohtani, K., Wakamiya, N.
  • Journal Title: (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47 II
  • Author(s): Koide, T., Nishikawa, Y., Asada, S., Yamazaki, C.M., Takahara, Y., Homma, D.L., Otaka, A., Ohtani, K., Wakamiya, N., Nagata, K., Kitagawa, K
  • Journal Title: J.Biol.Chem. (印刷中)
• [Journal Article] コレクチンとその免疫応答における役割
  • Author(s): 大谷克城, 鈴木定彦, 若宮伸隆
  • Journal Title: 臨床免疫 (印刷中)
• [Journal Article] Possibility of rapid detection for Variola virus using monoclonal antibody cross-reacted with Orthopoxviruses.
  • Author(s): Kitamoto, N., Kobayashi, T., Kato, Y., Wakamiya, N., Ikuta, K., Tanaka, T., Miyamoto, H., Kato, S.
  • Journal Title: Microbiol.Immunol. (印刷中)
• [Book] シンプル微生物学 (2006)
  • Author(s): 東匡伸, 小熊恵二編集(分担執筆)
  • Total Pages: 409
  • Publisher: 南山堂
  • Description: 「研究成果報告書概要(和文)」より
• [Book] シンプル微生物学 (2006)
  • Author(s): 東匡伸, 小熊恵二編集, 分担執筆
  • Publisher: 南山堂(印刷中)