| Project/Area Number |
16390161
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
WAKAMIYA Nobutaka Asahikawa Medical College, Faculty of Medicine, Professor, 医学部, 教授 (20210867)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUZAWA Jun Faculty of Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (40333695)
SUZUKI Yasuhiko Hokkaido University, Research Center for Zoonosis Control, Professor, 人獣共通感染症リサーチセンター, 教授 (90206540)
YOSHIDA Itsuro Asahikawa Medical College, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20041816)
OGASAWARA Masahiro Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (40185492)
OHTANI Katsuki Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (90396367)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | collectin / analysis / blood / 梗塞 / 炎症 / 定量的解析 |
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| Research Abstract |
CL-P1 is a new collectin which was found by Ohtani at 2001. It was expressed in vascular endothelial cells in murine tissues and also found in vascular endothelial cell lines. Here we established the antibody against above CL-P1 to investigate their biological functions. We have immunized mice with recombinant CL-P1 protein and made hybridoma for monoclonal antibodies. Several monoclonal antibodies were established and were divided to two or three groups. We tried to make the analysis method to detect CL-P1 using above monoclonal antibodies. We made several prototype assay systems but we could not get a good result because of less CL-P1 volume. Now we began to make a new analysis system using polyclonal antibodies.
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