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Investigation on protein adduct produced by alternatives to ozone-depleting substances and susceptibility genes and establishment of new method for risk assessment.

Research Project

Project/Area Number 16390169
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionNagoya University

Principal Investigator

ICHIHARA Gaku  Nagoya University Graduate School of Medicine, Occupational and Environmental Health, Associate Professor, 大学院医学系研究科, 助教授 (90252238)

Co-Investigator(Kenkyū-buntansha) KAMIJIMJA Michihiro  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (80281070)
NASU Tamie  Nagoya University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (10020794)
MAEDA Keiichiro  Nagoya University, Graduate School of Medicine, Professor, 大学院生命農学研究科, 教授 (30181580)
TSUKAMURA Hiroko  Nagoya University, Graduate School of Bioagricultural Sciences Biomodeling, Associate Professor, 大学院生命農学研究科, 助教授 (00212051)
IWAHASHI Hitoshi  National Institute of Advanced Industrial Science and Technology, Human Stress Signal Research Center, Senior Researcher, ヒューマンストレスシグナルセンター, 主任研究員 (60356540)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥10,200,000 (Direct Cost: ¥10,200,000)
Keywords1-bromopropane / protein adduct / susceptibility gene / dose-response relationship / neurotoxicity / microarray / mass spectrometry / risk assessment / フロン代替物質 / 感受性 / 曝露指標 / 遺伝子発現 / GST / LC-MS / グロビン蛋白 / F344 / Wistar
Research Abstract

1. The neuronal effects of 1-bromopropane in two inbred rat strains WNA (Wistar Nagoya) and F344 were compared to find genes related with susceptibility to 1-bromopropane exposure. Oligonucleotide microarray was applied to detect the difference of gene expression in two strains. The results showed that F344 is more susceptible than WNA in neuronal effects. The microarray study showed that glutathione S-transferase and epoxy hydrolase's expression were enhanced thorough two strains. Neuronal regeneration related gene was enhanced in WNA but suppressed in F344, showing a contrasting effect on two strains. Further investigation is needed to understand how this gene is related with the difference of susceptibility in two strains.
2. Metabolite N-acetyl S-propyl cysteine was detected with GC-MS and LC-MS/MS in the urine obtained from inhalation experiments using rats. This method is also applied to human samples successfully.
3. Protein adduct S-propyl cysteine was detected with LC-MS/MS in globin obtained from rats exposed to 1-bromopropane. This method is applied to human samples successfully.
4. Investigation on polymorphism of glutathione S-transferase clarify the dose responserelationship in the workers exposed to 1-bromopropane.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (34 results)

All 2007 2006 2005 2004 Other

All Journal Article (32 results) Book (2 results)

  • [Journal Article] A role for the aryl hydrocarbon receptor in regulation of ischemia-induced angiogenesis2007

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Arteriosclerosis Thrombosis and Vascular Biology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A role for the aryl hydrocarbon receptor in regulation of ischemia-induced angiogenesis2007

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Arteriosclerosis Thrombosis and Vascular Biology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pravastatin increases survival and suppresses an increase in myocardial matrix metalloproteinase activity in a rat model of heart failure.2006

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Cardiovasc Res 213

      Pages: 726-735

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of exposure of rat dams to 1-bromopropane during pregnancy and 1 actation on growth and sexual maturation of their offspring.2006

    • Author(s)
      Furuhashi K. et al.
    • Journal Title

      Toxicology 224

      Pages: 219-228

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] PPAR-alpha attenuates left ventricular remodeling and dysfunction by the suppression of the redox-regulated transcription factors activity.2006

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Journal of Molecular Cellular Cardiology 41

      Pages: 318-329

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Attenuation of oxidative stress and cardiac dysfunction by bisoprolol in an animal model of dilated cardiomyopathy.2006

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Biochem Biophys Res Commun 350

      Pages: 105-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of exposure of rat dams to 1-bromopropane during pregnancy and lactation on growth and sexual maturation of their offspring2006

    • Author(s)
      Furuhashi K. et al.
    • Journal Title

      Toxicology 224

      Pages: 219-228

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Ichihara G
    • Journal Title

      Nagai Shoten, Chudokucho no Subete. Kurokawa K. Edit Total

      Pages: 410-410

    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of exposure of rat dams to 1-bromopropane during pregnancy and lactation on growth and sexual maturation of their offspring.2006

    • Author(s)
      Furuhashi K. et al.
    • Journal Title

      Toxicology 224

      Pages: 219-228

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pravastatin increases survival and suppresses an increase in myoca rdial MMP activity in a rat model of heart failure.2006

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Cardiovascular Research 69

      Pages: 726-735

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Attenuation of oxidative stress and cardiac dysfunction by bisopro lol in an animal model of dilated cardiomyopathy.2006

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Biochem Biophys Res Commun 350

      Pages: 105-113

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pravastatin increases survival and suppresses an increase in myocardial matrix metalloproteinase activity in a rat model of heart failure.2006

    • Author(s)
      Ichihara S. et al.
    • Journal Title

      Cariovasc Res 213

      Pages: 726-735

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Neuro-reproductive toxicities of 1-bromopropane and 2-bro mopropane.2005

    • Author(s)
      Ichihara G.
    • Journal Title

      International Archives of Occupational and Environmental Health 78

      Pages: 79-96

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Species differences in the metabolism of di(2-ethylhexyl) phthalate (DEHP) in several organs of mice, rats, and marmosets2005

    • Author(s)
      Ito Y.et al.
    • Journal Title

      Archives of Toxicology 79

      Pages: 147-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 事例と動物実験からの毒性解明-ブロモプロパン研究を例として-2005

    • Author(s)
      市原 学
    • Journal Title

      日本衛生学雑誌 60

      Pages: 405-410

    • NAID

      10016884376

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] A comprehensive evaluation of the testicular toxicity of dichlorvos in Wistar rats.2005

    • Author(s)
      Okamura A. et al.
    • Journal Title

      Toxicology 213

      Pages: 129-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Neuro-reproductive toxicities of 1-bromopropane and 2-bromopropane.2005

    • Author(s)
      Ichihara G.
    • Journal Title

      International Archives of Occupational and Environmental Health 78

      Pages: 79-96

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Investigation of Toxicity through Human Cases and Animal Experiments-study of bromopropanes-2005

    • Author(s)
      Ichihara G. et al.
    • Journal Title

      Japanese Journal of Hygiene 60

      Pages: 405-410

    • NAID

      10016884376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comprehensive evaluation of the testicular toxicity of dichlorvos in Wistar rats.2005

    • Author(s)
      Okamura A. et al.
    • Journal Title

      Toxicology

      Pages: 129-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neuro-reproductive toxicities of 1-bromopropane and 2-bromopropane.2005

    • Author(s)
      Ichihara G
    • Journal Title

      International Archives of Occupational and Environmental Health 78

      Pages: 79-96

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Species differences in the metabolism of di(2-ethylhexyl) phthalate (DEHP) in several organs of mice, rats, and marmosets.2005

    • Author(s)
      Ito Y.et al.
    • Journal Title

      Archives of Toxicology 79

      Pages: 147-154

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A survey on exposure level, health status, and biomarkers in workers exposed to 1-bromopropane.2004

    • Author(s)
      Ichihara G. et al.
    • Journal Title

      American Journal of Industrial Medicine 45

      Pages: 63-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neurologic abnormalities in workers of a 1-bromopropane factory.2004

    • Author(s)
      Ichihara G. et al.
    • Journal Title

      Environmental Health Perspectives 112

      Pages: 1319-1325

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A survey of semen indices in insecticide sprayers.2004

    • Author(s)
      Kamijima M. et al.
    • Journal Title

      Journal of Occupational Health 46

      Pages: 109-118

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Serial alterations of beta-adrenergic signaling in dilated cardiomy opathic hamsters : possible role of myocardial oxidative stress.2004

    • Author(s)
      Nishizawa T. et al.
    • Journal Title

      Circulation Journal 68

      Pages: 1051-1060

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Serial alterations of beta-adrenergic signaling in dilated cardiomyopathic hamsters : possible role of myocardial oxidative stress.2004

    • Author(s)
      Nishizawa T. et al.
    • Journal Title

      Circulation Journal 68

      Pages: 1051-1060

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A survey on exposure level, health status, and biomarkers in workers exposed to 1-bromopropane.2004

    • Author(s)
      Ichihara G.et al.
    • Journal Title

      American Journal of Industrial Medicine 45

      Pages: 63-75

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Neurologic abnormalities in workers of a 1-bromopropane factory.2004

    • Author(s)
      Ichihara G.et al.
    • Journal Title

      Environmental Health Perspectives 112

      Pages: 1319-1325

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A survey of semen indices in insecticide sprayers.2004

    • Author(s)
      Kamijima M.et al.
    • Journal Title

      Journal of Occupational Health 46

      Pages: 109-118

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Serial alterations of beta-adrenergic signaling in dilated cardiomyopathic hamsters : possible role of myocardial oxidative stress.2004

    • Author(s)
      Nishizawa T.et al.
    • Journal Title

      Circulation Journal 68

      Pages: 1051-1060

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Neuro-reproductive toxicities of 1-bromopropane and 2-bromopropane.

    • Author(s)
      Ichihara G.
    • Journal Title

      International Archives of Occupational and Environmental Health (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Species differences in the metabolism of di(2-ethylhexyl) phthalate (DEHP) in several organs of mice, rats, and marmosets

    • Author(s)
      Ito Y.et al.
    • Journal Title

      Archives of Toxicology (in press)

    • Related Report
      2004 Annual Research Report
  • [Book] 黒川 顕編 中毒症のすべてーいざという時に役立つ、的確な治療のためにー2006

    • Author(s)
      市原 学
    • Total Pages
      410
    • Publisher
      永井書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 中毒症のすべて-いざという時に役立つ、的確な治療のために-(黒川 顕編)2006

    • Author(s)
      市原 学
    • Total Pages
      410
    • Publisher
      永井書店
    • Related Report
      2006 Annual Research Report 2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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