| Project/Area Number |
16390200
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Kyushu University |
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Principal Investigator |
KUBO Chiharu Kyushu University, Graduate School of Medical Sciences, Prof., 大学院医学研究院, 教授 (80117100)
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| Co-Investigator(Kenkyū-buntansha) |
SUDO Nobuyuki Kyushu University, Graduate School of Medical Sciences, Assoc.Prof., 大学院医学研究院, 助教授 (60304812)
KOGA Yasuhiro Tokai University, School of Medicine, Prof., 医学部, 教授 (60170221)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2004: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | Stress / Microbiota / Neuroinnune / 無菌マウス / 脳腸相関 |
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| Research Abstract |
Indigenous microbiota have several beneficial effects on host physiological functions ; however, little is known about whether or not postnatal microbial colonization can affect the development of brain plasticity and a subsequent physiological system response. In a series of experiments using germfree (GF), specific pathogen free (SPF), and gnotobiotic mice, we recently demonstrated that gut microbes could affect the development of hypothalamic-pituitary-adrenal (HPA) reaction to stress. In this study, in order to further verify these findings, we performed glucocorticoid-suppression test in GF and SPF mice. In addition, norepinephrine (NE), serotonin (5-HT), and y-aminobutyric acid (GABA) levels in various lesions of the brain were measured in both groups of mice. As a result, corticosterone pretreatment dose-dependently suppressed plasma ACTH response to restraint stress to a significantly lesser extent in GF mice than in SPF mice. GF mice exhibited increased NE and 5-HT levels in the cortex and hippocampus relative to SPF mice, although there was no difference in GABA concentration in any parts of the brain between either group of mice. These results further support the concept that the series of events in the gastrointestinal tract following postnatal microbial colonization can have a long-lasting impact on the neural processing of sensory information regarding the endocrine-stress axis.
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